Immunolocalization of humanin in human sperm and testis

Moretti, Elena; Giannerini, V.; Rossini, Lara; Matsuoka, Masao; Trabalzini, Lorenza; Collodel, Giulia

doi:10.1016/j.fertnstert.2010.04.075

Cited by 39 publications

(29 citation statements)

References 13 publications

(19 reference statements)

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“…Some studies have shown that the HN family peptides are present in hormone-dependent tissues such as testis [11], [14] and uterus [26]. Now, we detected HNr expression in anterior pituitary gland from both male and female rats.…”

Section: Discussionmentioning

confidence: 48%

“…HN is expressed in the endothelial cell layer of human arteries and veins [7] and in carotid atherosclerotic plaques [8]. HN has also been detected in human skeletal muscle [9], plasma [10], testis and sperm [11]. HNr was shown to be expressed in a number of adult rat tissues such as cerebral cortex, hippocampus, heart, testis and uterus, among others [12].…”

Section: Introductionmentioning

confidence: 99%

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Antiapoptotic Factor Humanin Is Expressed in Normal and Tumoral Pituitary Cells and Protects Them from TNF-α-Induced Apoptosis

et al. 2014

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Humanin (HN) is a 24-amino acid peptide with cytoprotective action in several cell types such as neurons and testicular germ cells. Rattin (HNr), a homologous peptide of HN expressed in several adult rat tissues, also has antiapoptotic action. In the present work, we demonstrated by immunocytochemical analysis and flow cytometry the expression of HNr in the anterior pituitary of female and male adult rats as well as in pituitary tumor GH3 cells. HNr was localized in lactotropes and somatotropes. The expression of HNr was lower in females than in males, and was inhibited by estrogens in pituitary cells from both ovariectomized female and orquidectomized male rats. However, the expression of HNr in pituitary tumor cells was not regulated by estrogens. We also evaluated HN action on the proapoptotic effect of TNF-α in anterior pituitary cells assessed by the TUNEL method. HN (5 µM) per se did not modify basal apoptosis of anterior pituitary cells but completely blocked the proapoptotic effect of TNF-α in total anterior pituitary cells, lactotropes and somatotropes from both female and male rats. Also, HN inhibited the apoptotic effect of TNF-α on pituitary tumor cells. In summary, our results demonstrate that HNr is present in the anterior pituitary gland, its expression showing sexual dimorphism, which suggests that gonadal steroids may be involved in the regulation of HNr expression in this gland. Antiapoptotic action of HN in anterior pituitary cells suggests that this peptide could be involved in the homeostasis of this gland. HNr is present and functional in GH3 cells, but it lacks regulation by estrogens, suggesting that HN could participate in the pathogenesis of pituitary tumors.

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Section: Discussionmentioning

confidence: 48%

Section: Introductionmentioning

confidence: 99%

Antiapoptotic Factor Humanin Is Expressed in Normal and Tumoral Pituitary Cells and Protects Them from TNF-α-Induced Apoptosis

et al. 2014

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“…[51718192021] Multiple studies have demonstrated that HN was cytoprotective against injury-induced apoptosis in many tissues including neuronal tissue,[522232425262728] blood-derived cells,[29] heart and blood vessels,[183031] pancreatic beta cells,[732] and testis. [91933] A very recent study provides new evidence for the “anti-inflammatory” effect of HN. A role of HN in the downregulation of inflammatory responses has been demonstrated in vivo and in cell culture systems.…”

Section: Discussionmentioning

confidence: 99%

Effects of humanin on experimental colitis induced by 2,4,6-trinitrobenzene sulphonic acid in rats

Gültekin

Emre

Çelik

et al. 2017

Saudi J Gastroenterol

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“…HN was first discovered in the surviving neurons of human Alzheimer's disease brain (Hashimoto et al ., 2001). Subsequently, HN was found to be cytoprotective against injury-induced apoptosis in many tissues including neuronal tissue (Hashimoto et al ., 2001; Kariya et al ., 2002, 2005; Nishimoto et al ., 2004; Matsuoka et al ., 2006; Xu et al ., 2006, 2010), blood-derived cells (Wang et al ., 2005), heart and blood vessels (Jung & Van Nostrand, 2003; Bachar et al ., 2010; Muzumdar et al ., 2010), pancreatic beta cells (Muzumdar et al ., 2009; Hoang et al ., 2010), and testis (Lue et al ., 2010; Moretti et al ., 2010; Jia et al ., 2013). HN has no reported action on normal cells not under stress.…”

Section: Introductionmentioning

confidence: 99%

“…HN was identified as one of the most frequently expressed genes in the primary spermatocytes from the human testis (Liang et al ., 2004), and in human spermatozoa (Moretti et al ., 2010). HN protected immature Leydig cell from apoptosis induced by dexamethasone in young rats (Colon et al ., 2006).…”

Section: Introductionmentioning

confidence: 99%

Humanin protects against chemotherapy-induced stage-specific male germ cell apoptosis in rats

et al. 2015

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SUMMARY Humanin (HN) has cytoprotective action on male germ cells after testicular stress induced by heat and hormonal deprivation. To examine whether HN has protective effects on chemotherapy-induced male germ cell apoptosis, we treated four groups of adult rats with (i) vehicle (control), (ii) HN, (iii) cyclophosphamide (CP); or (iv) HN+CP. To investigate whether the protective effects of HN on germ cells require the presence of Leydig cells, another four groups of rats were pre-treated with ethane dimethanesulfonate (EDS), a Leydig cell toxicant, to eliminate Leydig cells. After 3 days, when Leydig cells were depleted by EDS, we administered: (i) vehicle, (ii) HN, (iii) CP; or (iv) HN+CP to rats. All rats were killed 12 h after the injection of HN and/or CP. Germ cell apoptosis was detected by TUNEL assay and quantified by numerical count. Compared with control and HN (alone), CP significantly increased germ cell apoptosis; HN +CP significantly reduced CP-induced apoptosis at early (I–VI) and late stages (IX–XIV) but not at middle stages (VII–VIII) of the seminiferous epithelial cycle. Pre-treatment with EDS markedly suppressed serum and intratesticular testosterone (T) levels, and significantly increased germ cell apoptosis at the middle (VII–VIII) stages. CP did not further increase germ cell apoptosis in the EDS-pre-treated rats. HN significantly attenuated germ cell apoptosis at the middle stages in EDS pre-treated rats. To investigate whether HN has any direct effects on Leydig cell function, adult Leydig cells were isolated and treated with ketoconazole (KTZ) to block testosterone synthesis. HN was not effective in preventing the reduction of T production by KTZ in vitro. We conclude that HN decreases CP and/or EDS-induced germ cell apoptosis in a stage-specific fashion. HN acts directly on germ cells to protect against EDS-induced apoptosis in the absence of Leydig cells and intratesticular testosterone levels are very low.

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Immunolocalization of humanin in human sperm and testis

Cited by 39 publications

References 13 publications

Antiapoptotic Factor Humanin Is Expressed in Normal and Tumoral Pituitary Cells and Protects Them from TNF-α-Induced Apoptosis

Antiapoptotic Factor Humanin Is Expressed in Normal and Tumoral Pituitary Cells and Protects Them from TNF-α-Induced Apoptosis

Effects of humanin on experimental colitis induced by 2,4,6-trinitrobenzene sulphonic acid in rats

Humanin protects against chemotherapy-induced stage-specific male germ cell apoptosis in rats

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