Immunolocalization of Ghrelin in the Stomach of Sprague‐Dawley Rat

Li, J.; Yi, Shuang-Qin; Wang, H.-X.; Yi, Nozomi; Ogawa, Yujiro; Ozaki, Noriyuki; Itoh, Masahiro

doi:10.1111/j.1439-0264.2010.01044.x

Cited by 6 publications

(4 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gastric acid secretion is subjected to an important hormonal regulation. The bulk of ghrelin in the body occurs in the A-like cells of the acid-producing part of the stomach, and plasma ghrelin-like immunoreactivity levels in totally gastrectomized patients were reduced to 35% of those in normal controls (Li et al, 2011), which suggested that ghrelin played the important role in regulating gastric acid secretion.…”

Section: Introductionmentioning

confidence: 99%

RNAi-mediated Ghrelin affects gastric H+–K+-ATPase activity and expression of GOAT–Ghrelin system in vitro

Luo

et al. 2016

General and Comparative Endocrinology

View full text Add to dashboard Cite

Ghrelin has been implicated in the regulation of gastric functional development, and its physiological functions are mediated by Ghrelin-O-acyltransferase (GOAT) which is capable of generating the active form of this polypeptide hormone. However, whether and how ghrelin gene silencing may modify gastric acid secretion and GOAT-Ghrelin system is yet to be explored. The study was performed in gastric mucosal cells from weanling piglets in vitro. We evaluated the effect of ghrelin on gastric acid secretion, gene expression of GOAT and ghrelin as well as ghrelin levels by RNA interference assay. shGhrelin triggered the down-regulation of ghrelin mRNA expression (P<0.05) via an RNAi mechanism, as observed by real-time RT-PCR. In addition, shGhrelin showed reduced total ghrelin production and secretion (P<0.05) using ELISA in vitro. We also detected that GOAT mRNA expression was reduced in shGhrelin group (P<0.05), compared with control groups. In accordance with the GOAT expression, acylated ghrelin production and secretion were reduced in gastric mucosal cells and culture medium (P<0.05). Silencing of ghrelin gene achieved by RNAi-mediation inhibited the activity of H(+)-K(+)-ATPase and pepsin (P<0.05) in gastric mucosal cells. These results indicated that RNAi of Ghrelin gene inhibited the gastric acid secretion with decreased GOAT mRNA and acylated Ghrelin in gastric mucosal cells.

show abstract

Section: Introductionmentioning

confidence: 99%

RNAi-mediated Ghrelin affects gastric H+–K+-ATPase activity and expression of GOAT–Ghrelin system in vitro

Luo

et al. 2016

General and Comparative Endocrinology

View full text Add to dashboard Cite

show abstract

“…Ghrelin increases food intake and decreases energy expenditure, leading to a positive energy balance [15]. There was a potential use of ghrelin for gastrointestinal diseases [12] and temperature regulation [16]. The pharmacologic actions of ALZ involved energy charges [9, 10] and ATPase activity [11].…”

Section: Discussionmentioning

confidence: 99%

“…The immunohistochemical localization of ghrelin was detected according to the method described by Li et al [12] with some modification. After the tissue sections (6 μ m) were treated with 3% normal goat serum for 1 h at 25°C, then rabbit polyclonal antibody (Santa Cruz) against rat ghrelin was applied to the gastric sections (1 : 200) and incubated overnight.…”

Section: Methodsmentioning

confidence: 99%

Underlying Mechanism of Aconitum Lizhong Acting on Experimental Hypothermia with Indigestion in Rats: Role of Ghrelin

Zhao

Wang

Yang

et al. 2012

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

This study is aimed to investigate the Aconitum Lizhong pill (ALZ) pharmacological actions on hypothermia with indigestion, especially the ghrelin roles. The littermate-matched rats were randomly divided into four groups. Control did sham operation or standard diet, Model carried out interscapular brown adipose (IBA) removal with standard diet, Fat-diet did IBA removal with fat-diet, and ALZ did IBA removal and fat-diet with 4.536 g/kg/d ALZ. The potency of adaptive thermogenesis, ghrelin levels in plasma or gastric mucosa, thyroid hormones and metabolite in sera, expression of ghrelin mRNA, and protein in gastric mucous membrane were determined. ALZ relieved the hypothermia processes with indigestion, via inhibiting ghrelin expression and increasing ghrelin secretion; the dynamics from the therapy is supported with the energy changes as less body weight loss, less plasma lipid decrease, more plasma T 3 or T 4 increase with TSH decrease, and more compensation of thermogenic AUC decrease. Ghrelin played key roles in the actions of ALZ on the hypothermia with indigestion. The pharmacological mechanisms of ALZ involved the homeostasis of ghrelin expression and secretion.

show abstract

“…The tissues were thawed, cut into small pieces and kept at 4 °C in a glass container containing 4 volumes of cold HB. The tissues were homogenised five times with a Polytron PT1200 (Kinematica AG, Switzerland) and then centrifuged at 12 000 g for 30 min at 4 °C . The supernatant was collected with care to avoid fat contamination, aliquoted and stored at −70 °C until used.…”

Section: Methodsmentioning

confidence: 99%

Daily Patterns and Adaptation of the Ghrelin, Growth Hormone and Insulin‐Like Growth Factor‐1 System Under Daytime Food Synchronisation in Rats

Arellanes-Licea¹,

Báez-Ruíz²,

Carranza³

et al. 2014

J Neuroendocrinology

View full text Add to dashboard Cite

Daytime restricted feeding promotes the re-alignment of the food entrained oscillator (FEO). Endocrine cues which secretion is regulated by the transition of fasting and feeding cycles converge in the FEO. The present study aimed to investigate the ghrelin, growth hormone (GH) and insulin-like growth factor (IGF)-1 system because their release depends on rhythmic and nutritional factors, and the output from the system influences feeding and biochemical status. In a daily sampling approach, rats that were fed ad lib. were compared with rats on a reversed (daytime) and restricted feeding schedule by 3 weeks (dRF; food access for 2 h), also assessing the effect of acute fasting and refeeding. We undertook measurements of clock protein BMAL1 and performed somatometry of peripheral organs and determined the concentration of total, acylated and unacylated ghrelin, GH and IGF-1 in both serum and in its main synthesising organs. During dRF, BMAL1 expression was synchronised to mealtime in hypophysis and liver; rats exhibited acute hyperphagia, stomach distension with a slow emptying, a phase shift in liver mass towards the dark period and decrease in mass perigonadal white adipose tissue. Total ghrelin secretion during the 24-h period increased in the dRF group as a result of elevation of the unacylated form. By contrast, GH and IGF-1 serum concentration fell, with a modification of GH daily pattern after mealtime. In the dRF group, ghrelin content in the stomach and pituitary GH content decreased, whereas hepatic IGF-1 remained equal. The daily patterns and synthesis of these hormones had a rheostatic adaptation. The endocrine adaptive response elicited suggests that it may be associated with the regulation of metabolic, behavioural and physiological processes during the paradigm of daytime restricted feeding and associated FEO activity.

show abstract

Immunolocalization of Ghrelin in the Stomach of Sprague‐Dawley Rat

Cited by 6 publications

References 22 publications

RNAi-mediated Ghrelin affects gastric H+–K+-ATPase activity and expression of GOAT–Ghrelin system in vitro

RNAi-mediated Ghrelin affects gastric H+–K+-ATPase activity and expression of GOAT–Ghrelin system in vitro

Underlying Mechanism of Aconitum Lizhong Acting on Experimental Hypothermia with Indigestion in Rats: Role of Ghrelin

Daily Patterns and Adaptation of the Ghrelin, Growth Hormone and Insulin‐Like Growth Factor‐1 System Under Daytime Food Synchronisation in Rats

Contact Info

Product

Resources

About