Immunolocalization of FsPK1 correlates this abscisic acid-induced protein kinase with germination arrest in Fagus sylvatica L. seeds

Abstract

An enzymatically active recombinant protein kinase, previously isolated and characterized in Fagus sylvatica L. dormant seeds (FsPK1), was used to obtain a specific polyclonal antibody against this protein. Immunoblotting and immunohistochemical analysis of FsPK1 protein in beech seeds showed a strong immunostaining in the nucleus of the cells located in the vascular tissue of the embryonic axis corresponding to the future apical meristem of the root. This protein kinase was found to accumulate in the seeds only when embryo growth was arrested by application of ABA, while the protein amount decreased during stratification, previously proved to alleviate dormancy, and no protein was detected at all when seed germination was induced by addition of GA(3). These results indicate that FsPK1 may be involved in the control of the embryo growth mediated by ABA and GAs during the transition from dormancy to germination in Fagus sylvatica seeds.

Effect of Foliar Spray with IAA and GA3 on Production and Protein Synthesis of Two Summer Squash Hybrid Cultivars

“…In this instance, H 2 (Eskandrani F 1 ) showed the missing of 4 bands (53.4, 58.6, 61.7, 173.4 KD) by GA 3 treatment. Similar results were also obtained by Reyes et al (2006) and Mekki (2008). The early study of Jacobsen and Knox (1974) showed that GA 3induced 12 proteins in barley leaves ranged from 15.5 to 81.0 KD, while only two new proteins were detected in aleurone layers.…”

“…Also, the results indicated that exogenous IAA and GA 3 changes gene expression to induce several proteins involved in various developmental processes in squash plants. Similar conclusion was also obtained by O'Neill and Scorr (1987), Abel et al(1994), Ramos et al (2001), Hagen and Guilfoyle (2002), Reyes et al (2006), Mekki (2008), Maraschin et al (2009) and Zanjan & Asli (2014).…”

Desiccation tolerance acquisition in developing beech (Fagus sylvatica L.) seeds: the contribution of dehydrin-like protein

“…However, in cotyledons, the protein is detected later, possibly due to the lower ABA concentration. In beech seeds several components have been identified to participate in ABA signal transduction pathway, like protein kinases FsPK1 (Reyes et al 2006b) and FsPK4 (Jiménez et al 2006), protein phosphatase 2C FsPP2C2 (Reyes et al 2006a). Detection of more diverse factors involved in ABA signaling and possible interactions between them is needed to understand the complicated mechanism.…”

Caracterización y análisis funcional de dos proteínas relacionadas con la regulación hormonal de la dormición y germinación de semillas de haya

“…También en nuestro laboratorio se han aislado y caracterizado ciertas proteína quinasas (FsPK1, FsPK2 y FsPK4) inducidas por ABA y probablemente relacionadas con la dormición de semillas de haya (Lorenzo et al, 2000;Jiménez et al, 2006;Reyes et al, 2006b).…”

“…Así por ejemplo, se ha comprobado que 2 proteína fosfatasa de tipo 2C, FsPP2C1 y FsPP2C2, participarían como reguladores de la ruta del ABA y controlarían la transición entre dormición/germinación de semillas (González-García et al, 2003;Reyes et al, 2006a). También mediante una aproximación farmacológica, se ha demostrado la importancia de las desfosforilación en restos de tirosina en el control del crecimiento postgerminativo mediado por ABA (Reyes et al, 2006b) Con estos antecedentes y debido a la imposibilidad de realizar transformaciones genéticas en haya, hemos utilizado las herramientas genéticas disponibles en Arabidopsis para la obtención de plantas transgénicas, así como mutantes con pérdida de función que nos permitirán realizar un análisis funcional de los genes seleccionados en los procesos de señalización hormonal relacionados con diferentes aspectos de la fisiología de semillas. Para ello, nos planteamos los siguientes objetivos:…”