Immunolocalization of CD44 and the Ezrin–Radixin–Moesin (ERM) Family in the Stratum Intermedium and Papillary Layer of the Mouse Enamel Organ

Nakamura, Hiroaki; Ozawa, Hiroki

doi:10.1177/002215549704501105

Cited by 15 publications

(12 citation statements)

References 57 publications

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“…The evidence that preameloblasts cannot diVerentiate into secretory ameloblasts without stratum intermedium cells suggests that cell-cell-interactions play an essential role in the diVerentiation process of ameloblasts (Sakakura et al 1989). Several transmembrane proteins such as the gap junction protein connexin 43 and the cell adhesion receptor CD44 take part in the cross-talk between stratum intermedium cells and ameloblasts (Nakamura and Ozawa 1997;Joao and Arana-Chavez 2003). Therefore, we suggest that heterotypic interactions between cells of the stratum intermedium and those of the ameloblastic layer take part in EMMPRIN-induced expression of MMPs in ameloblasts.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemical and biochemical detection of EMMPRIN in the rat tooth germ: differentiation-dependent co-expression with MMPs and co-localization with caveolin-1 in membrane rafts of dental epithelial cells

Schwab

Harada

Goetz

et al. 2007

Histochem Cell Biol

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In tooth development matrix metalloproteinases (MMPs) are under the control of several regulatory mechanisms including the upregulation of expression by inducers and downregulation by inhibitors. The aim of the present study was to monitor the occurrence and distribution pattern of the extracellular matrix metalloproteinase inducer (EMMPRIN), the metalloproteinases MMP-2 and MT1-MMP and caveolin-1 during the cap and bell stage of rat molar tooth germs by means of immunocytochemistry. Strong EMMPRIN immunoreactivity was detected on the cell membranes of ameloblasts and cells of the stratum intermedium in the bell stage of the enamel organ. Differentiating odontoblasts exhibited intense EMMPRIN immunoreactivity, especially at their distal ends. Caveolin-1 immunoreactivity was evident in cells of the internal enamel epithelium and in ameloblasts. Double immunofluorescence studies revealed a focal co-localization between caveolin-1 and EMMPRIN in ameloblastic cells. Finally, western blotting experiments demonstrated the expression of EMMPRIN and caveolin-1 in dental epithelial cells (HAT-7 cells). A substantial part of EMMPRIN was detected in the detergent-insoluble caveolin-1-containing low-density raft membrane fraction of HAT-7 cells suggesting a partial localization within lipid rafts. The differentiation-dependent co-expression of MMPs with EMMPRIN in the enamel organ and in odontoblasts indicates that EMMPRIN takes part in the induction of proteolytic enzymes in the rat tooth germ. The localization of EMMPRIN in membrane rafts provides a basis for further investigations on the role of caveolin-1 in EMMPRIN-mediated signal transduction cascades in ameloblasts.

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Section: Discussionmentioning

confidence: 99%

Immunocytochemical and biochemical detection of EMMPRIN in the rat tooth germ: differentiation-dependent co-expression with MMPs and co-localization with caveolin-1 in membrane rafts of dental epithelial cells

Schwab

Harada

Goetz

et al. 2007

Histochem Cell Biol

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“…The interaction between the membrane skeleton and cytoplasmic domains of transmembrane proteins plays a fundamental role in membrane integrity and stability, as well as in many other cellular processes [19]. In addition, the CD44 cytoplasmic domain also can directly bind to ERM family members (ezrin, radixin, and moesin), which are crucial components that provide a regulated linkage between membrane proteins and the cortical cytoskeleton, as well as participating in signal-transduction pathways [6, 20, 21]. Our in vivoresults showed that CD44 was expressed distinctly on the lateral PM and in close contact with lateral PM between cells (cell-cell adhesion) in the middle stage of epithelial cell repair after ATN.…”

Section: Discussionmentioning

confidence: 99%

Different Type and Localization of CD44 on Surface Membrane of Regenerative Renal Tubular Epithelial Cells in vivo

et al. 2004

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Background: CD44 is a transmembrane glycoprotein comprising an extracellular domain, a transmembrane domain, and a cytoplasmic tail. Previous studies demonstrated that CD44 was generally restricted to lateral-basal plasma membrane (PM) of epithelial cells, whether it localized on apical PM in vivo has not been clarified. Methods: In this study, we used a gentamicin-induced acute tubular necrosis (ATN) and spontaneous recovery model in rats and two distinct antibodies, an anti-rat distal extracellular domain (OX49) of standard CD44 (CD44-OX49) and an anti-rat CD44 cytoplasmic tail (CD44CPT), to survey the localization of CD44-OX49 and CD44CPT on the PM in renal tubular epithelial cells in different recovery stages after ATN with immunohistochemistry and immunoelectron-microscopic examinations. Results: CD44-OX49 was localized not only on the lateral-basal PM in tubular epithelial cells, but also on the apical surface membrane in PCNA-positive newly regenerative tubular epithelial cells in early recovery stages after ATN. However, CD44CPT was only localized on the lateral-basal PM. The immunoelectron-microscopic results showed that CD44-OX49 localization was changed from the apical to lateral to basal surface membrane in renal tubular epithelial cells during the recovery process after ATN, finally disappearing from basal PM when normal polarized epithelial cells formed. Conclusions: These results suggest that there were two types of CD44 including CD44 without a cytoplasmic tail localizing on the apical surface membrane related to newly regenerative epithelial cells, and CD44 with a cytoplasmic tail localizing on the lateral-basal PM related to establishment of tubular epithelial cell polarity after ATN in vivo.

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“…From ED16 to ED19, the IDE becomes a monolayer and the number of IDE cells in contact with both the SI and the BM increases. Change in cell-cell interactions may intervene when the IDE differentiates and becomes a monolayer [17]. This reorganization is still in progress at ED17 in the molar where three types of cells were identified in the IDE: cells that are in contact either with the BM (a) or the SI (b) and cells that interact with both the BM and the SI (c) ( Figures 3A, 3B).…”

Section: Enlargement Of the Enamel Organ At The Bell Stagementioning

confidence: 97%

“…The IDE and SI cells were stained by anti E-cadherin antibodies. CD44, a transmembrane protein involved in cell-matrix and cell-cell interactions as well as signal transduction, also seems to mediate interactions between the SI and preameloblasts [17]. Cell-cell interactions between the SI and preameloblastsameloblasts do play a mechanical role and might also be involved in the cytodifferentiation of ameloblasts [18].…”

Section: Introductionmentioning

confidence: 99%

Cell-Cell and Cell-Matrix Interactions During Initial Enamel Organ Histomorphogenesis in the Mouse

Lesot

Kieffer-Combeau

Fausser³

et al. 2002

Connective Tissue Research

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Relationships between cell-cell/cell-matrix interactions and enamel organ histomorphogenesis were examined by immunostaining and electron microscopy. During the cap-bell transition in the mouse molar, laminin-5 (LN5) disappeared from the basement membrane (BM) associated with the inner dental epithelium (IDE), and nondividing IDE cells from the enamel knot (EK) underwent a tooth-specific segregation in as many subpopulations as cusps develop. In the incisor, the basement membrane (BM) in contact with EK cells showed strong staining for LN5 and integrin alpha 6 beta 4. LN5 seems to provide stable adhesion, while its proteolytic processing might facilitate cell segregation. In both teeth, immunostaining for antigens associated with desmosomes or adherens junctions was similar for EK cells and neighboring IDE cells. Outside the EK, IDE cell-BM interactions changed locally during the initial molar cusp delimitation and on the labial part of the incisor cervical loop. Conversely, cell-cell junctions stabilized the anterior part of the incisor during completion of morphogenesis. Time and space regulation of cell-matrix and cell-cell interactions might thus play complementary roles in allowing plasticity during tooth morphogenesis and stabilization at later stages of epithelial histogenesis.

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Immunolocalization of CD44 and the Ezrin–Radixin–Moesin (ERM) Family in the Stratum Intermedium and Papillary Layer of the Mouse Enamel Organ

Cited by 15 publications

References 57 publications

Immunocytochemical and biochemical detection of EMMPRIN in the rat tooth germ: differentiation-dependent co-expression with MMPs and co-localization with caveolin-1 in membrane rafts of dental epithelial cells

Immunocytochemical and biochemical detection of EMMPRIN in the rat tooth germ: differentiation-dependent co-expression with MMPs and co-localization with caveolin-1 in membrane rafts of dental epithelial cells

Different Type and Localization of CD44 on Surface Membrane of Regenerative Renal Tubular Epithelial Cells in vivo

Cell-Cell and Cell-Matrix Interactions During Initial Enamel Organ Histomorphogenesis in the Mouse

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