Immunolabeling of carbonic anhydrase isoenzyme C and glial fibrillary acidic protein in paraffin-embedded tissue sections of human brain and retina.

Kumpulainen, Timo; Dahl, D.; Korhonen, L. Kalevi; Nyström, S. H. M.

doi:10.1177/31.7.6406590

Cited by 76 publications

(19 citation statements)

References 32 publications

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“…Immunochemical studies identified CA II as the most widely distributed CA in the eye (15)(16)(17)(18). It is especially abundant in epithelial cells of the ciliary process, in Muller cells of the retina, and in a subset of cone photoreceptor cells.…”

mentioning

confidence: 99%

Localization of carbonic anhydrase IV in a specific capillary bed of the human eye.

Hageman¹,

Zhu²,

Waheed³

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

123

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Carbonic anhydrase (CA) activity plays an important role in controlling aqueous humor production in the eye and in regulating intraocular pressure. Prior studies identified the soluble isozymes CA II and CA I in the human eye and also suggested a distinct membrane-associated CA. We used an antibody to CA IV, the membrane-anchored isozyme from human lung, to study CA IV in eye tissues and to compare its distribution with that of CA II. We found intense immunostaining for CA IV associated with endothelial cells of one specific uveal capillary bed, the choriocapillaris. CA IV was not detected in endothelial cells of the contiguous capillaries of the iris or in endothelial cells of other vessels. Immunoreactivity for CA IV was also intense in epithelial and fiber cells of the lens but was not detectable in the neuroretina, the ciliary process (except for capillaries), and the cornea, all sites where immunostaining with anti-CA II antibody was intense. These studies indicate that the membrane-associated CA in human eye, which was suspected from histochemical studies, is CA IV. Defining the physiological role of this ocular isozyme remains a challenge.

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mentioning

confidence: 99%

Localization of carbonic anhydrase IV in a specific capillary bed of the human eye.

Hageman¹,

Zhu²,

Waheed³

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

123

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“…Anti-GFAP staining has been used to investigate the distribution of glia in the normal retina of several species including man. [5][6][7] Correspondence to Dr P. Hiscott We compared the stability of the GFAP-containing Specimens for study by immunofluorescent staining were placed in OCT embedding media (Lab-Tek products, Illinois) and immediately frozen in liquid nitrogen. Sections 71xm thick were cut in a cryostat from eight of the peeled membranes, and portions were air dried on to glass slides for 20 minutes.…”

mentioning

confidence: 99%

Retinal and epiretinal glia--an immunohistochemical study.

Hiscott¹,

Grierson²,

Trombetta³

et al. 1984

British Journal of Ophthalmology

195

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“…The localization of CA II is well documented in the normal human oligodendrocytes [43]. Based on our previous studies, CA IX is not present in the normal human brain [16].…”

Section: Discussionmentioning

confidence: 99%

The tumour-associated carbonic anhydrases CA II, CA IX and CA XII in a group of medulloblastomas and supratentorial primitive neuroectodermal tumours: an association of CA IX with poor prognosis

et al. 2010

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BackgroundMedulloblastomas (MBs) and supratentorial primitive neuroectodermal tumours (PNETs) are the most common highly aggressive paediatric brain tumours. In spite of extensive research on these tumours, there are only few known biomarkers or therapeutic target proteins, and the prognosis of patients with these tumours remains poor. Our aim was to investigate whether carbonic anhydrases (CAs), enzymes commonly overexpressed in various tumours including glioblastomas and oligodendrogliomas, are present in MBs and PNETs, and whether their expression can be correlated with patient prognosis.MethodsWe determined the expression of the tumour-associated carbonic anhydrases CA II, CA IX and CA XII in a series of MB/PNET specimens (n = 39) using immunohistochemistry.ResultsEndothelial CA II, cytoplasmic CA II, CA IX and CA XII were expressed in 49%, 73%, 23% and 11% of the tumours, respectively. CA II was detected in the neovessel endothelium and the tumour cell cytoplasm. CA IX was mainly expressed in the tumour cells located in perinecrotic areas. CA XII showed the most homogenous distribution within the tumours. Importantly, CA IX expression predicted poor prognosis in both univariate (p = 0.041) and multivariate analyses (p = 0.016).ConclusionsWe suggest that CA IX should be considered a potential prognostic and therapeutic target in MBs and PNETs.

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Immunolabeling of carbonic anhydrase isoenzyme C and glial fibrillary acidic protein in paraffin-embedded tissue sections of human brain and retina.

Cited by 76 publications

References 32 publications

Localization of carbonic anhydrase IV in a specific capillary bed of the human eye.

Localization of carbonic anhydrase IV in a specific capillary bed of the human eye.

Retinal and epiretinal glia--an immunohistochemical study.

The tumour-associated carbonic anhydrases CA II, CA IX and CA XII in a group of medulloblastomas and supratentorial primitive neuroectodermal tumours: an association of CA IX with poor prognosis

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