Immunoinformatics analysis and evaluation of recombinant chimeric triple antigen toxoid (r-HAB) against Staphylococcus aureus toxaemia in mouse model

Kota, Rohini Krishna; Kolla, Harish Babu; Reddy, Prakash Narayana; Kalagatur, Naveen Kumar; Samudrala, Siva Kumar

doi:10.1007/s00253-021-11609-z

Cited by 8 publications

(6 citation statements)

References 44 publications

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“…The cytotoxic effects of SeNPs on both normal and cancerous cell populations were measured using the MTT assay, which was determined by the cellular proliferation rate and cell viability (Swaminathan et al, 2019(Swaminathan et al, , 2021Kota et al, 2021). The methodology is provided in the Supplementary Data [(10) Cell viability and micro-morphology assessment].…”

Section: Cell Viability and Micro-morphology Assessmentmentioning

confidence: 99%

Multi-Biofunctional Properties of Phytofabricated Selenium Nanoparticles From Carica papaya Fruit Extract: Antioxidant, Antimicrobial, Antimycotoxin, Anticancer, and Biocompatibility

Vundela

Kalagatur²,

Nagaraj

et al. 2022

Front. Microbiol.

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The present study focused on phytofabrication of selenium nanoparticles (SeNPs) from Carica papaya extract and exploration of their multi-biofunctional features. Total phenolics and flavonoids of C. papaya fruit extract were determined as 23.30 ± 1.88 mg gallic acid equivalents and 19.21 ± 0.44 mg quercetin equivalents per gram, respectively, which suggested that C. papaya fruit extract could be a competitive reducing and stabilizing agent during phytofabrication of nanoparticles. UV–Vis and FTIR spectroscopy showed the formation of SeNPs from sodium selenite, which could be related to the reducing and stabilizing activities of C. papaya fruit extract. The SeNPs were found to be stable with a Zeta potential of −32 mV. The average hydrodynamic size of SeNPs was found as 159 nm by dynamic light scattering. The SeNPs showed a broader XRD pattern with no sharp Bragg’s peaks and found to be amorphous. SEM showed that SeNPs were spherical in shape and EDX pattern showed that SeNPs were made up of Se (71.81%), C (11.41%), and O (14.88%). The HR-TEM picture showed that SeNPs were spherical in morphology and have a size range of 101–137 nm. The SeNPs exhibited potent antioxidant activity and their EC50 values (effective concentration required to inhibit 50% of radicals) were 45.65 ± 2.01 and 43.06 ± 3.80 μg/ml in DPPH and ABTS assays, respectively. The antimicrobial action of SeNPs was found as a broad spectrum and suppressed microbial pathogens in ascending order: fungi > Gram-positive bacteria > Gram-negative bacteria. The SeNPs have been demonstrated to reduce the growth and ochratoxin A (OTA) of mycotoxigenic Aspergillus ochraceus and Penicillium verrucosum at 40 μg/ml in broth culture, which is noteworthy. The SeNPs reduced cancer cell proliferation (RAW 264.7, Caco-2, MCF-7, and IMR-32) more preferentially than normal cells (Vero), found to be highly biocompatible. Lower doses of SeNPs (up to 50 μg/ml) were shown to be less toxic and did not cause death in Danio rerio (zebrafish) embryos, implying that lower doses of SeNPs could be beneficial for biological purposes. The present study concluded that phytofabricated SeNPs have multiple biofunctional properties, including antioxidant, antimicrobial, antimycotoxin, and anticancer activities, as well as high biocompatibility.

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Section: Cell Viability and Micro-morphology Assessmentmentioning

confidence: 99%

Multi-Biofunctional Properties of Phytofabricated Selenium Nanoparticles From Carica papaya Fruit Extract: Antioxidant, Antimicrobial, Antimycotoxin, Anticancer, and Biocompatibility

Vundela

Kalagatur²,

Nagaraj

et al. 2022

Front. Microbiol.

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“…Because the OmpL of S. typhimurium which we considered for analysis is highly conserved among other Enterobacteriaceae pathogens in terms of its sequence and structure, this protein can be used as a broad-spectrum recombinant subunit vaccine for the gastrointestinal infections caused by the Enterobacteriaceae pathogens. Similar to our previous reports on subunit vaccine development, the OmpL can be cloned and expressed and can be administered to mice along with the potential adjuvants like Freund’s adjuvants to induce strong immunogenicity [ 8 , 9 , 14 ].…”

Section: Discussionmentioning

confidence: 87%

Mapping of conserved immunodominant epitope peptides in the outer membrane porin (Omp) L of prominent Enterobacteriaceae pathogens associated with gastrointestinal infections

Kolla,

Makam,

Reddy

2023

Journal of Genetic Engineering and Biotechnology

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Background Members of Enterobacteriaceae such as Escherichia coli O 157:H7, Salmonella sp., Shigella sp., Klebsiella sp., and Citrobacter freundii are responsible for the outbreak of serious foodborne illness and other mucosal infections across the globe. The outer membrane proteins (OMPs) of Enterobacteriaceae are highly immunogenic in eliciting immune responses against pathogens. Moreover, the OMPs are highly conserved in the Enterobacteriaceae family. Sequence homology in the OMPs will ensure the presence of conserved immunodominant regions with predominant epitopes. The OmpL is such an immunogen that is highly conserved among the Enterobacteriaceae pathogens. In this study, we performed computational analysis on the outer membrane porin (Omp) L of prominent Enterobacteriaceae pathogens. Results Multiple sequence and structural alignment analysis have revealed that the OmpL protein is highly conserved among the selected Enterobacteriaceae pathogens. This amount of sequence and structural homology uncovered the conserved antibody binding B-cell epitopes in the OmpL protein. The B-cell epitopes predicted in the OmpL of Salmonella typhimurium are highly conserved among the other Enterobacteriaceae pathogens. Conclusion In conclusion, these conserved B-cell epitopes will vouch for the generation of heterologous humoral immune response in conferring cross protection against the Enterobacteriaceae pathogens and control their outbreaks across the globe.

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“…Liver models were assembled by staggered seeding of vascular and hepatic cell layers. In each sterilized biochip, 2.7x10 5 HUVECs/cm 2 and 1x10 5 monocytes/cm 2 were mixed and seeded on top of the membrane. HUVEC/monocytes were cocultured for five days with a daily medium exchange with vascular perfusion medium: endothelial cell medium supplemented with 20% autologous human serum, 10 ng/ml human granulocyte-macrophage colonystimulation factor (GM-CSF) (Peprotech), and 10 ng/ml human macrophage colony-stimulating factor (M-CSF) (Peprotech) to induce macrophage differentiation.…”

Section: Liver-on-chipmentioning

confidence: 99%

“…Infections with S. aureus are thus a leading cause of death in developed countries [3], with the MRSA strain USA300 reaching pandemic status across North America [4]. To date, no approved vaccine for S. aureus infections is available [5].…”

Section: Introductionmentioning

confidence: 99%

Human macrophage polarization determines bacterial persistence ofStaphylococcus aureusin a liver-on-chip-based infection model

Siwczak

Cseresnyés

Carlstedt

et al. 2021

Preprint

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Infections with Staphylococcus aureus (S. aureus) have been reported from various organs ranging from asymptomatic colonization to severe infections and sepsis associated with multiple organ dysfunction. Although considered an extracellular pathogen, S. aureus can invade and persist in professional phagocytes such as monocytes and macrophages. Its capability to persist and manipulate phagocytes is considered a critical step to evade host antimicrobial reactions. For the first time we leveraged a human liver-on-chip model and tailored image analysis algorithms to demonstrate that S. aureus (USA300) specifically targets macrophages in the liver models as essential niche facilitating bacterial persistence and phenotype switching to small colony variants (SCVs). In vitro M2 polarization was found to favor SCV-formation and was associated with increased intracellular bacterial loads in macrophages, increased cell death, and impaired recruitment of circulating monocytes to sites of infection. These findings expand the knowledge about the role of liver macrophages in the course of systemic infection. Further, the results might be relevant for understanding infection mechanisms in patients with chronic liver disease such as fibrosis that display increased frequencies of M2 polarized liver macrophages and have a higher risk for developing chronic infections and relapsing bacteremia.

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Immunoinformatics analysis and evaluation of recombinant chimeric triple antigen toxoid (r-HAB) against Staphylococcus aureus toxaemia in mouse model

Cited by 8 publications

References 44 publications

Multi-Biofunctional Properties of Phytofabricated Selenium Nanoparticles From Carica papaya Fruit Extract: Antioxidant, Antimicrobial, Antimycotoxin, Anticancer, and Biocompatibility

Multi-Biofunctional Properties of Phytofabricated Selenium Nanoparticles From Carica papaya Fruit Extract: Antioxidant, Antimicrobial, Antimycotoxin, Anticancer, and Biocompatibility

Mapping of conserved immunodominant epitope peptides in the outer membrane porin (Omp) L of prominent Enterobacteriaceae pathogens associated with gastrointestinal infections

Human macrophage polarization determines bacterial persistence ofStaphylococcus aureusin a liver-on-chip-based infection model

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