Immunohistomorphometric Changes of The Pituitary Gonadotropic Cells After Testosterone Application in a Rat Model of the Andropause

Ristić, Nataša; Ajdžanović, Vladimir; Petrovic-Kosanovic, Dragana; Miler, Marko; Ušćebrka, Gordana; Milošević, Verica

doi:10.2478/macvetrev-2018-0023

Cited by 1 publication

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“…These effects of the anabolic steroid administration are caused by the negative feedback mechanism. Similar findings were reported by Ristić et al (2019). They showed that testosterone administration decreased volume density and also optical density of pituitary gonadotropic cells in orhidectomized rats.…”

Section: Discussionsupporting

confidence: 92%

“…They showed that testosterone administration decreased volume density and also optical density of pituitary gonadotropic cells in orhidectomized rats. Furthermore, they detected that the increased levels of testosterone in orhidectomized rats led to a decrease of size and number of gonadotropic cells in the pituitary gland, which is, in our opinion, direct confirmation that the sex hormone had a crucial influence on both function and morphometric parameters of gonadotropic cells by the mechanism of negative feedback (Ristić et al, 2019). In our study, serum levels of LH and FSH hormones were decreased after treatment with nandrolone decanoate alone or combined with swimming training.…”

Section: Discussionmentioning

confidence: 53%

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Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats

et al. 2020

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The aim of the study was to investigate the effects of chronic nandrolone decanoate treatment and/or swimming training on immunohistomorphometric parameters on rat pituitary gonadotropic cells. Male Wistar albino rats, 10 weeks old, were classified into four groups: control (T−N−), nandrolone (T−N+), swimming training (T+N−), and swimming training with nandrolone (T+N+). The T+ groups swam for 4 weeks, 1 h/day, 5 days/week. The N+ groups received nandrolone decanoate (20 mg/kg) once per week for 4 weeks. Pituitary tissue sections were processed and stained for immunohistochemical analysis and immunofluorescence. The volume density of luteinizing hormone (LH) cells was decreased by 48% in T−N+ and for 35% in the T+N+ group. The volume density of follicle-stimulating hormone (FSH) cells was decreased by 39% in T−N+ and for 30% in T+N+ compared to the control. Nandrolone alone, or combined with swimming training, decreased the number of LH/FSH cells compared to the control. The levels of the immunofluorescent signal of LH/FSH cells were increased in all experimental groups. Nandrolone alone decreased the serum level of LH by 17%, whereas swimming training alone increased FSH levels by 11% compared to the control. Serum levels of testosterone were increased in all experimental groups. Nandrolone alone, or combined with swimming training, decreased immunohistomorphometric parameters of gonadotropic cells, whereas the levels of immunofluorescent signal were increased.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 53%