Immunohistological study to determine the presence of pericryptal myofibroblasts and basement membrane in colorectal epithelial tumors

Higaki, Shingo; Tada, Masahiro; Nishiaki, Makoto; Mitani, Noriyuki; Yanai, Hideo; Okita, Kiwamu

doi:10.1007/s005350050246

Cited by 7 publications

(6 citation statements)

References 27 publications

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“…Thus, there is a correlation between PCFS and epithelial cell differentiation. This is similar to the previous reports that a significant reverse correlation was seen between the degree of colorectal neoplasia progression and the quantity of neoplastic glands with PCFS [3,19] . Above findings demonstrated that PCFS significantly influences the differentiation of epithelial cells via epithelial-mesenchymal interaction.…”

Section: Discussionsupporting

confidence: 91%

Pericryptal fibroblast sheath in intestinal metaplasia, dysplasia and carcinoma of the stomach

Liu

Sun

et al. 2009

Chin. J. Cancer Res.

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Objective:To investigate the existence of pericryptal fibroblasts sheath (PCFS) in normal gastric mucosa, intestinal metaplasia (IM), indefinite for dysplasia (I-Dys), low grade dysplasia (L-Dys), high grade dysplasia (H-Dys) and gastric cancer (GC), and its association with gastric carcinogenesis.Methods: In this study, we examined the existence of PCFS in normal gastric mucosa (N=10), IM (N=26), I-Dys (N=16), L-Dys (N=13), H-Dys (N=21) and GC (N=145) using immunohistochemical staining for two smooth muscle markers, alpha smooth muscle actin(α-SMA) and high molecular weight caldesmon (h-CD). The significance of PCFS was discussed, especially in association with gastric carcinogenesis.Results: The PCFS was recognized in 65.4%(17/26) of IM, 62.5%(10/16) of I-Dys and 23.1% (3/13) of L-Dys respectively. No PCFS was detected in H-Dys and GC. The PCFS was gradually reduced in IM, Dys and GC in sequence (P<0.0001).Conclusion: The PCFS is associated with the differentiation of epithelium and involved in gastric carcinogenesis via epithelial-mesenchymal interaction.

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Section: Discussionsupporting

confidence: 91%

Pericryptal fibroblast sheath in intestinal metaplasia, dysplasia and carcinoma of the stomach

Liu

Sun

et al. 2009

Chin. J. Cancer Res.

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“…Yao and Tsuneyoshi 14 revealed that, in the intramucosal lesions of colorectal cancer, PMF disappeared around the cancer cell nests, while in the submucosal lesions, the complete loss of PMF was observed around the cancer cell nests. Higaki et al 27 . reported that in the distribution of PMF around the tumor, there was no difference between adenoma with low‐grade dysplasia and the normal colon.…”

Section: Discussionmentioning

confidence: 99%

Differential distribution of basement membrane type IV collagen α1(IV), α2(IV), α5(IV) and α6(IV) chains in colorectal epithelial tumors

Hiki

Iyama

Tsuruta

et al. 2002

Pathology International

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In this study, we examined the relationship between the histopathological grade and immunohistochemical localization of six genetically distinct type IV collagen alpha chains, the major component of basement membrane (BM), in normal and neoplastic colorectal tissues. In the normal colorectal mucosa, alpha1/alpha2(IV) and alpha5/alpha6(IV) chains were stained in all epithelial BM. However, alpha3/alpha4(IV) chains were restrictively immunostained in the BM of the apical surface epithelium. Similar immunostaining profiles for alpha1/alpha2(IV) and alpha5/alpha6(IV) chains were observed in tubular adenomas with mild/moderate atypia. However, in intramucosal carcinomas, both alpha1/alpha2(IV) chains were linearly stained in the BM of cancer cell nests, while the assembly of alpha5/alpha6(IV) chains into the BM was inhibited in a discontinuous or negatively stained pattern. The normal colorectal mucosa forms a second network of BM composed of alpha5/alpha6(IV), partly alpha3/alpha4(IV) chains, in addition to the classic network of alpha1/alpha2(IV) chains. The differential immunohistochemical localization of the type IV collagen alpha5/alpha6 chains could be one diagnostic marker for the invasiveness of colorectal cancer.

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“…PCFs are thought to drive epithelial differentiation, produce components of the basement membrane, and secrete paracrine factors [27,28]. α-SMA and HHF-35 immunoreactive PCFs decrease along the adenoma-carcinoma sequence [27,29,30].…”

Section: Discussionmentioning

confidence: 99%

Histochemical localization of caldesmon isoforms in colon adenocarcinoma and lymph node metastases

Köhler

2011

Virchows Arch

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Alternative splicing of the caldesmon gene results in high (h-caldesmon) and low (l-caldesmon) molecular weight isoforms of the cytoskeleton-associated protein caldesmon. h-Caldesmon is predominantly expressed not only in smooth-muscle cells but also in pericryptal fibroblasts in colon. l-Caldesmon is widely expressed and localized in podosomes/invadopodia. Studies with transformed and cancer cell lines suggest that a reduction in l-caldesmon facilitates podosome/invadopodia formation and metastasis. We investigated caldesmon isoforms in colon adenocarcinoma and their lymph node metastases using immunohistochemistry. Caldesmon immunoreactivity of colon adenocarcinoma primary tumors and lymph node metastases was very similar. l-Caldesmon immunoreactivity of cancer epithelial cells in primary tumors and lymph node metastases varied ranging from reduced to stronger as compared to immunoreactivity of normal areas. The variation did not show a consistent relation to the tumor center or invasive margin. While h-caldesmon immunoreactivity of pericryptal fibroblasts and blood vessels in the stroma of primary tumors and lymph node metastases was markedly reduced, cancer-associated fibroblasts and blood vessels in the stroma were strongly immunoreactive for l-caldesmon. Our results show a differential behavior of h- and l-caldesmon isoforms in epithelium and stroma of colon adenocarcinoma and lymph node metastases.

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Immunohistological study to determine the presence of pericryptal myofibroblasts and basement membrane in colorectal epithelial tumors

Cited by 7 publications

References 27 publications

Pericryptal fibroblast sheath in intestinal metaplasia, dysplasia and carcinoma of the stomach

Pericryptal fibroblast sheath in intestinal metaplasia, dysplasia and carcinoma of the stomach

Differential distribution of basement membrane type IV collagen α1(IV), α2(IV), α5(IV) and α6(IV) chains in colorectal epithelial tumors

Histochemical localization of caldesmon isoforms in colon adenocarcinoma and lymph node metastases

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