Immunohistological determination of interleukin-1β in inflamed human gingival epithelium

Hillmann, Georg; Hillmann, Beate; Geurtsen, Werner

doi:10.1016/0003-9969(94)00136-y

Cited by 20 publications

(12 citation statements)

References 18 publications

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“…Numerous reports have described increased levels of these cytokines in crevicular fluid and periodontal tissues of patients with periodontitis (Masada et al 1990, Rossomando et al 1990, Stashenko et al 1991, Tokoro et al 1996, Roberts et al 1997, and there is evidence that antagonists of IL-1 and TNF can inhibit bone loss associated with induced periodontitis in animal models (Assuma et al 1998). The source of these cytokines in the inflamed periodontium includes several cell types (Agarwal et al 1995, Hendley et al 1995, Hillmann et al 1995, Takahashi et al 1995, Galbraith et al 1997. In our studies we have focused on cytokine production by PMN, since these cells constitute the largest population in gingival crevicular fluid, and synthesize significant amounts of both IL-1b and TNF-a in situ (Hendley et al 1995, Galbraith et al 1997.…”

Section: Discussionmentioning

confidence: 99%

Polymorphic cytokine genotypes as markers of disease severity in adult periodontitis

Galbraith

Hendley

Sanders

et al. 1999

J Clinic Periodontology

127

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The distributions of the bi-allelic interleukin-1beta+3953 and tumor necrosis factor-alpha-308 genotypes were determined in 20 patients with advanced adult periodontitis, 20 patients with plaque associated gingivitis, and 45 referent population subjects. A significant increase in IL-1beta+3953 allele 2 frequency was found in patients with advanced periodontitis compared to referent subjects (the Fisher exact test; p=0.013). Furthermore, the frequency of TNF-alpha-308 allele 1 was significantly greater in patients with advanced disease compared to those with plaque associated gingivitis (the Fisher exact test; p=0.014). No significant correlation was observed between genotype and cytokine production in these patient populations.

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Section: Discussionmentioning

confidence: 99%

Polymorphic cytokine genotypes as markers of disease severity in adult periodontitis

Galbraith

Hendley

Sanders

et al. 1999

J Clinic Periodontology

127

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“…Activated keratinocytes within the pocket and epithelial-resident Langerhans cells, which are of monocytic lineage, secrete IL-la and IL-lß. 173 Monocytic cells within the inflamed periodontal tissues secrete predominantly IL-1 ß.174 175 Bacterial LPS is a potent stimulus for IL-1 secretion.…”

Section: Ful2329mentioning

confidence: 99%

Periodontal Diseases: Pathogenesis

1996

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“…Diagnosis and classification of AP was made according to previously defined criteria24 based on physical examination, probing depth, tooth mobility, and observation of bleeding upon probing, and supported by full-mouth series of intraoral radiographs. According to this classification, the total patient group included 11 subjects with early Periodontitis, 14 with moderate disease, and 15 with advanced Periodontitis. Subjects recruited into the control group were determined to be free of periodontal disease based on history and clinical examination alone; radiography was not performed on control subjects.…”

Section: Subjectsmentioning

confidence: 99%

Cytokine Production by Oral and Peripheral Blood Neutrophils in Adult Periodontitis

Galbraith

Hagan²,

Steed

et al. 1997

Journal of Periodontology

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Proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) also possess bone-resorptive properties, and are generally considered to play a role in the pathogenesis of periodontal disease. In the present study, TNF-alpha and IL-1 beta production by oral and peripheral blood polymorphonuclear leukocytes (PMN) was examined in 40 patients with adult periodontitis and 40 orally healthy matched controls. Oral PMN released considerable amounts of both cytokines in unstimulated culture, and there was no difference between patients and controls when the cytokine levels were corrected for cell number. However, when the effect of disease activity was examined, cytokine release by oral PMN was found to be greatest in patients with advanced periodontitis. Within the healthy control group, IL-1 beta production by oral PMN was significantly higher in males (Mann-Whitney test, P = 0.0008). Examination of IL-1 beta production by peripheral blood PMN exposed to recombinant human granulocyte-macrophage colony stimulating factor revealed no difference between the patient and control groups. In contrast, IL-1 beta production by peripheral blood PMN was significantly reduced in patients with advanced disease (Mann-Whitney test, P = 0.02), and peripheral PMN IL-1 beta synthesis was greater in female controls (Mann-Whitney test, P = 0.054). No effect of race on cytokine production could be discerned in patients or controls. These results indicate that several factors influence cytokine production in oral health and disease, and that a dichotomy in cytokine gene expression exists between oral and peripheral blood PMN in adult periodontitis.

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Immunohistological determination of interleukin-1β in inflamed human gingival epithelium

Cited by 20 publications

References 18 publications

Polymorphic cytokine genotypes as markers of disease severity in adult periodontitis

Polymorphic cytokine genotypes as markers of disease severity in adult periodontitis

Periodontal Diseases: Pathogenesis

Cytokine Production by Oral and Peripheral Blood Neutrophils in Adult Periodontitis

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