Immunohistological characterization of intraepithelial and lamina propria lymphocytes in control ileum and colon and in inflammatory bowel disease

Abstract

Using monoclonal antibodies to T and B lymphocytes, to natural killer cells, and to HLA-DR antigen, we characterized the lymphocyte population within the epithelial and lamina propria regions in control intestine and colon, and in grossly involved and in grossly uninvolved intestine and colon of patients with active inflammatory bowel disease. There were significantly more intraepithelial T cells in control ileum than in control colon. In comparison to control, there was a heterogeneity of alterations in intraepithelial and lamina propria T lymphocyte subsets (T11+, T8+, T4+) in inflammatory bowel disease. B lymphocytes were not detected within the lamina propria, except when found in and adjacent to lymphoid aggregates. Leu 7+ cells were uncommon in the lamina propria of control ileum and colon and in diseased tissues. The majority of intraepithelial lymphocytes did not express HLA-DR. Epithelial cells of control colon did not express HLA-DR while epithelial cells of control ileal tissues and of diseased colonic and ileal specimens expressed HLA-DR antigen. Only small numbers of lamina propria T cells expressed HLA-DR in both control and disease tissues. There was intense expression of HLA-DR by monocytes and modest expression of HLA-DR by capillary and lymphatic endothelial cells. The induction of HLA-DR expression by diseased colonic epithelium and the observation that lymphatic endothelium expresses HLA-DR are new observations, and we established that Leu 7+ cells are present in very small numbers in both normal and diseased intestine and colon.

Changes of the Peptide YY Levels in the Intestinal Tissue of Rats with Experimental Colitis following Oral Administration of Mesalazine and Prednisolone

“…Currently, it is reported that UC symptoms arise due to a speciˆc autoimmune reaction in the large intestine. 21) However, despite many reports on UC research, the determining factor for UC progression has not been clariˆed. Therefore various studies have been performed using UC animal models in attempts to elucidate the cause of UC and establish a treatment for it.…”

Histopathological Features of the Terminal Ileum in Lymphocytic and Collagenous Colitis: A Study of 32 Cases and Review of Literature

“…In normal subjects, 80 -90% of colonic and ileal intraepithelial lymphocytes are T cells (9,10), and 5-40% of these are of the ␥␦ subtype (11). Mosnier et al (12) showed that the colonic intraepithelial lymphocytes in lymphocytic and collagenous colitis are CD3ϩ/CD8ϩ T cells.…”

Colonic neuropathological aspects in patients with intractable constipation due to obstructed defecation

“…44 In fact, experimental human and animal studies have shown that enteric glial cells may also be involved in intestinal inflammation, 45,46 enteric neurotransmission [47][48][49] and, importantly, are essential for the homeostasis of enteric neurons. 50 With respect to this last aspect, a recent study showed that enteric glia disruption may alter the neurochemical coding of enteric neurons in an experimental animal model.…”