Immunohistological and morphometric analysis of inflammatory cells in rapidly progressive periodontitis and adult periodontitis

Abstract

The purpose of this study was to localize, characterize, and quantify in situ the inflammatory cells in the gingival connective tissue prior and subsequent to the initial therapy of ten patients with rapidly progressive periodontitis (RPP) and five patients with adult periodontitis (AP). Using immunohistological techniques, the amount of T lymphocytes, alphabeta-T lymphocytes, gammadelta-T lymphocytes, B lymphocytes, and plasma cells was determined at the beginning of the periodontal therapy (baseline) and at the time of periodontal surgery. Furthermore, the distribution of collagen types I, III, V, and VI was investigated using transmission electron microscopy. At baseline, patients with RPP revealed much higher numbers of inflammatory cells than patients with AP. During initial therapy of patients with RPP, the amount of T cells, alphabeta-T cells, and gammadelta-T cells was reduced significantly (P<0.05). Biopsies of patients with AP revealed a statistically significant reduction of all cell types, except alphabeta-T cells and gammadelta-T cells in the deep connective tissue. The transmission electron microscopy of biopsies from patients with RPP and AP with severe inflammation taken at baseline revealed that collagen types I and III were destroyed nearly completely in areas with leukocyte infiltration, whereas collagen types V and VI revealed a more pronounced labeling reaction. The results revealed that, during initial therapy, the amount of inflammatory cells was reduced significantly more in biopsies of patients with AP than in patients with RPP. At baseline, the inflamed gingival tissue consists mainly of collagen types V and VI in areas with infiltrates of inflammatory cells.

Morphometric analysis of collagen and inflammatory cells in periodontal disease

“…Our finding are similar to previous reports 4,8,14 and taking into account data from all studies, the mean collagen volume density in the healthy gingiva was in the range from 54% to 63%, in gingivitis cases from 38% to 46%, and in the specimens from severe parodontopathy from 25% to 27%. In our study, with the progression of the disease, gingival collagen volume density decreases, with the loss of collagen fibers within inflammatory foci and its increase around the infiltrates.…”

A Three-Dimensional Multivariate Image Processing Technique for the Analysis of FTIR Spectroscopic Images of Multiple Tissue Sections

“…As inflammatory cell infiltration and subsequent collagen degradation are hallmarks of periodontitis [7][8][9][10], and because IR spectral analyses can determine such tissue events, in addition to multiple other tissue changes at the molecular and sub-molecular level, then this IR microspectroscopic methodology can now be applied to hypothesis-driven research that aims to identify diseaserelated pathological changes in periodontal tissues. Figure 7 Cluster mapping of periodontal tissue.…”

“…Inflammation-driven collagen degradation is a hallmark of periodontitis [7,8], with strong dissolution of collagen types I and III observed [9,10]. Particular absorptions are assigned to various functional groups in an attempt to extract biochemical information.…”

The presence, function and regulation of IL‐17 and Th17 cells in periodontitis

“…As well as neutrophils, gingival cd T cells have been shown to produce IL-17 and to play a potential role in tissue inflammation and alveolar bone destruction in experimental PD (Eskan et al 2012). In humans, the presence and frequency of gingival cd T cells in patients with PD has been investigated (Champaiboon et al 2000, Hillmann et al 2001. In humans, the presence and frequency of gingival cd T cells in patients with PD has been investigated (Champaiboon et al 2000, Hillmann et al 2001.…”