Immunohistological Analysis of Serial Biopsies Taken During Human Renal Allograft Rejection

Ww, Hancock; D, Gee; P, de Moerloose; Fr, Rickles; Va, Ewan; Rc, Atkins

doi:10.1097/00007890-198504000-00018

Cited by 62 publications

(11 citation statements)

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“…[13] T suppressor-cytotoxic CD8+ cells in a diffuse pattern and with invasion of tubular wall were considered the preponderant cells in acute rejection. [6,11] Macrophages [7,14] comprised between 30-60% of the total infiltrating leukocytes in renal allograft rejection. Variable numbers of B lymphocytes (2-10%) and null cells (2-30%) have been demonstrated in cases of Figure 2.…”

Section: Discussionmentioning

confidence: 99%

“…Once these cells are activated, they are capable of producing and releasing cytokines, which are critical as regulators of the immune response. [2,6,7] The immunophenotyping of biopsy specimens has confirmed the predominance of macrophages and T cells in interstitial infiltrates. The receptors for interleukin-2 are essential for lymphocyte interaction, cell growth, and new synthesis of other cytokines.…”

Section: Introductionmentioning

confidence: 95%

“…[6,7] Hancock et al [7] showed a transient increase in NK/K cell infiltration at days 2 and 3 post-transplant.…”

Section: Control Borderlinementioning

confidence: 99%

“…Some studies have reported greater number of CD4, CD8, and null cells in the initial stages of inflammatory process, with later increase of macrophages. [7,14] An examination of sequential biopsies should provide additional insight into the rejection process. [7] In human studies as well as in the rat model of acute renal allograft rejection, the number and proportion of macrophages were found to increase as rejection progressed, such that macrophages were the dominant cell type present during severe rejection at day 14.…”

Section: Control Borderlinementioning

confidence: 99%

“…[7,14] An examination of sequential biopsies should provide additional insight into the rejection process. [7] In human studies as well as in the rat model of acute renal allograft rejection, the number and proportion of macrophages were found to increase as rejection progressed, such that macrophages were the dominant cell type present during severe rejection at day 14. [14] The correlation between mononuclear cell infiltrates and outcome has been less clear.…”

Section: Control Borderlinementioning

confidence: 99%

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In Situ Expression of Mononuclear Cell Markers and Interleukin-2 Receptor in Renal Allograft Biopsies of Acute Rejection and Borderline Cases

et al. 2007

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The correct diagnosis of renal allograft rejection may be difficult using only clinical and/or histopathological criteria. Immunological assays should be considered in order to evaluate the phenotype of inflammatory infiltrate in renal allograft biopsies. Immunohistochemical studies were performed to detect mononuclear cells, CD4 and CD8 T lymphocytes, B lymphocytes, macrophages, null cells, and positive cells for interleukin-2 receptors. A total of 41 allograft biopsies classified into three groups were studied: acute cellular rejection (28 biopsies/22 patients), borderline (7 biopsies/5 patients) and control (6 biopsies/6 patients).In the rejection group (RG), increased cellularity was found mainly at the tubulo-interstitial level. Expression of CD8 positive cells was higher in RG when compared to borderline (BG) and control (CG) groups, respectively (0.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

“…[6,7] Hancock et al [7] showed a transient increase in NK/K cell infiltration at days 2 and 3 post-transplant.…”

Section: Control Borderlinementioning

confidence: 99%

Section: Control Borderlinementioning

confidence: 99%

Section: Control Borderlinementioning

confidence: 99%

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In Situ Expression of Mononuclear Cell Markers and Interleukin-2 Receptor in Renal Allograft Biopsies of Acute Rejection and Borderline Cases

et al. 2007

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CD40 engagement induces monocyte procoagulant activity through an interleukin‐10 resistant pathway

et al. 1996

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Interactions between membrane-bound molecules were previously shown to be involved in the induction of tissue factor-dependent monocyte procoagulant activity (PCA) by activated T cells. To investigate the potential role of the CD40/CD40 ligand (CD40L) pathway in this process, we first determined the effects of blocking anti-CD40 or anti-CD40L monoclonal antibodies (mAb) on the development of monocyte PCA during mixed lymphocyte reaction (MLR) between allogeneic peripheral blood mononuclear cells (PBMC). The strong inhibitory effect exerted by both mAb (mean percentages of inhibition: 88 and 91% for anti-CD40 and anti-CD40L mAb, respectively) indicates that CD40/CD40L interactions are required for the induction of PCA in MLR. These data led us to measure monocyte PCA after incubation of PBMC or purified monocytes with a stimulating anti-CD40 mAb (BL-C4) or with 3T6 fibroblasts transfected with the gene encoding CD40L. In both systems, we found that CD40 engagement strongly induced monocyte PCA which was related to tissue factor expression as shown by flow cytometric analysis. Finally, we observed that recombinant interleukin (IL)-10, which inhibits lipopolysaccharide-induced PCA, did not significantly influence CD40-dependent PCA. We conclude that CD40 engagement on monocytes induces tissue factor-dependent PCA through an IL-10-resistant pathway. These findings have implications for the control of coagulation events triggered by interactions between T cells and monocytes.

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Natural Cytotoxicity and Allograft Rejection

Häyry

Nemlander

Tarkkanen

et al. 1989

Functions of the Natural Immune System

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Immunohistological Analysis of Serial Biopsies Taken During Human Renal Allograft Rejection

Cited by 62 publications

References 0 publications

In Situ Expression of Mononuclear Cell Markers and Interleukin-2 Receptor in Renal Allograft Biopsies of Acute Rejection and Borderline Cases

In Situ Expression of Mononuclear Cell Markers and Interleukin-2 Receptor in Renal Allograft Biopsies of Acute Rejection and Borderline Cases

CD40 engagement induces monocyte procoagulant activity through an interleukin‐10 resistant pathway

Natural Cytotoxicity and Allograft Rejection

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