Immunohistologic Studies on Subpopulations of Lymphocytes in Cattle with Enzootic Bovine Leukosis

Abstract

The distribution of subpopulations of lymphocytes in lymph nodes and tumors from cattle with enzootic bovine leukosis (EBL) was examined by immunohistochemistry using a panel of monclonal antibodies against leukocyte differentiation molecules of EBL. The lesions in lymph nodes could be divided into three types based on the extent of infiltration and proliferation of neoplastic cells with provirus and differential expression of leukocyte differentiation molecules. The number of B-B2+, sIgM+ cells was reduced in frequency in follicles during the neoplastic cell proliferation. CD4- and CD8-positive alpha/beta T cells and gamma/delta T cells positive for WC1 (workshop cluster designation) were also reduced in frequency in areas infiltrated with neoplastic cells. Almost all neoplastic cells were B-B2- and IgM-positive. However, there were a few B-B2- and/or IgM-negative cells or cells stained faintly in all cases. WC1+ cells were not observed in tumor tissues. However, CD4+ and CD8+ cells were observed throughout tumor tissues, suggesting a role for these cells in tumor immunity.

Multicentric lymphoma in buffaloes in the Amazon region, Brazil

“…The histopathology is characterized by the proliferation of medium to large cells, lymphocytic and lymphoblastic in origin and with low mitotic indices. These cells have the phenotype of B lymphocytes, and necrotic areas are observed in the tumor masses [ 17 , 18 ].…”

Immunohistochemical Analysis of Expression Patterns of Tumor Necrosis Factor Receptors on Lymphoma Cells in Enzootic Bovine Leukosis

“…However, these neoplastic B cells are classified into different phenotypes by immunohistochemical analysis using a monoclonal antibody to leukocyte differentiation antigen [6,7,13,42,43,46]. Furthermore, tumor cells in EBL were classified into three or more types on the basis of histological findings [17,39].…”

An investigation of enzootic bovine leucosis (EBL) infection by agar gel immunodiffusion (AGID), enzyme linked immunosorbent assay (ELISA) tests and hematological applications on the dairy cows in the

“…The clinical appearance of lymphosarcoma in the herd or in the slaughterhouse constitutes a confirmation of the clinical suspicion. However, in the past, before the virological aetiology was discovered and of reliable laboratory diagnostic methods developed, histological and haematological keys were the only diagnostic tool for the detection of infected animals (Chiba et al, 1995). Nowadays, serological tests developed for the diagnosis of BLV infection include AGID, ELISA, complement fixation, radioimmunoassay and virus neutralization tests (Mammerickx et al, 1985).…”