Immunohistologic analysis of mycobacterial antigens by monoclonal antibodies in tuberculosis and mycobacteriosis

Beyond Hematoxylin and Eosin—The Role of Immunohistochemistry in Surgical Pathology

“…[41,42] Furthermore, similar results can be obtained with cheaper specialized histochemical stains such as the Kinyoun and Fite stains. [41,42] Similarly, histochemical stains have proven to be as effective as immunohistochemistry for the detection of fungi. [43] On the other hand, immunohistochemistry is diagnostically more practical for the detection of viruses.…”

Species-Specific Immunocytochemical Localization of Mycobacterium tuberculosis Complex in Fine Needle Aspirates of Tuberculous Lymphadenitis Using Antibody to 38 kDa Immunodominant Protein Antigen

“…To the best of our knowledge, only 2 published reports in the English-language literature have described ICC with monoclonal antibodies in tissue sections of tuberculosis and none in FNA smears (Table VI). Barbolini et al 8 observed that of the 4 monoclonal antibodies raised in mice against different proteins of MTB, antibody 61.3 was species specific for the 35 kDa protein of MTB with 100% positivity and was not reactive to Mycobacterium kansasii. In the second published report, by Mustafa et al 17 in 2006, the immunostaining was done on histologic sections of 55 cases of granulomatous lymphadenitis using monoclonal antibody to MPT64 and a sensitivity of 100% with specificity of 83% was observed.…”

Can an Immunohistochemistry Method Differentiate Intestinal Tuberculosis from Crohn’s Disease in Biopsy Specimens?

“…There have been some reports on IHC staining about establishing the diagnosis of M. tuberculosis [15][16][17][18][19][20][21][22][23][24] but most of them had used polyclonal antibodies, resulting in false-positive reactions due to antigenic cross reaction with other bacteria and fungi and only completed in tuberculosis patients with or without healthy controls. There is only one study in the English-language literature evaluating immunohistochemical staining with species-specific monoclonal antibody to 38-kDa antigen of M. tuberculosis complex in archival formalin-fixed paraffin-embedded tissue sections of extra-pulmonary tuberculosis [17].…”