Immunohistochemistry of primary gastrointestinal lymphomas: a study of 76 cases

Abstract

A retrospective study of 76 primary gastrointestinal lymphomas utilizing an avidin: biotinylated horseradish peroxidase complex (ABC) technique demonstrated 22 B-cell lymphomas, including two associated with alpha-heavy chain disease. Seven cases were classified as true histiocytic lymphomas based on a positive reaction for one or more of three histiocytic enzyme markers utilized, predominantly alpha-1-antitrypsin and alpha-1-antichymotrypsin. However, in 20 cases, an intense admixture of reactive histiocytes was noted and these cells stained preferentially for the enzyme, lysozyme. Twenty cases, which stained for both kappa and lambda light chains and positively or negatively for albumin, could not be classified and 27 cases failed to stain with any of the antisera utilized.

Gastrointestinal lymphomas - A clinicopathological study in a tertiary care centre

“…This study utilised CD 20 as a Bcell marker. The finding of most common gastrointestinal lymphomas are of Bcell type, being compatible with other studies [11][12][13][14] (Table 6). The most common clinical presentation noted was abdominal pain (9/12), being compatible with TADASHI TERADA STUDIES 3 (15/37).…”

Gastrointestinal lymphoma

“…These processes destroy surface immunoglobulins and phenotypic characterization [ 141. Immunoperoxidase staining is usually positive for B lymphocyte surface markers [26,28]. A combined analysis of cases from Stanford and UCLA noted that 84% were of B cell origin versus only 8% of T cell origin [29].…”

Alpha-1 anti-trypsin and CD30 expression occur in parallel in activated T cells

“…On this basis, al anti-trypsin was used as a marker to differentiate between histiocytic and lymphoid neoplasms [10][11][12]. The specificity of al anti-trypsin as a marker for histiocytes was subsequently called into question by reports of at anti-trypsin positive T cell tumours [13][14][15].…”