Immunohistochemistry, glycosylation and immunosuppression of glycodelin in human ovarian cancer

Jeschke, Udo; Mylonas, Ioannis; Kunert‐Keil, Christiane; Stahn, Renate; Scholz, Christoph; Janni, Wolfgang; Kühn, Christina; Schröder, Eike; Mayr, Doris; Friese, Klaus

doi:10.1007/s00418-008-0510-z

Cited by 17 publications

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References 56 publications

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“…Additionally, ascites Gd inhibits IL-2 stimulated proliferation of peripheral blood leucocytes and inhibits adhesion of SLeX-positive cells to E-selectin. Therefore, Gd could act as an inhibitor of lymphocyte activation and/or adhesion in ovarian cancer [23]. …”

Section: Discussionmentioning

confidence: 99%

Glycodelin A is a prognostic marker to predict poor outcome in advanced stage ovarian cancer patients

et al. 2012

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BackgroundGlycodelin is a cell surface glycoprotein offering a unique gender specific carbohydrate configuration. Sialylated carbohydrate structures, which are unusual for mammals, characterize Glycodelin isolated from amniotic fluid (Glycodelin A, GdA). Glycodelin in general exerts multiple, partly opposing functions ranging from immunosuppression to cell differentiation. As these markedly influence tumorigenesis, this study aimed to clarify whether expression of different Glycodelin isoforms is related to clinicopathological characteristics and prognosis of ovarian cancer patients. Further the use of Glycodelin as a serum marker in benign and malignant ovarian diseases was evaluated.MethodsOvarian cancer specimens (n = 152) were stained for Glycodelin with carbohydrate and peptide specific antibodies. Associations between Glycodelin expression and histological grading, FIGO stage as well as patient’s prognosis were examined. Glycodelin was correlated to expression of gonadotropin receptors and mucin-1, which are discussed as ovarian cancer tissue markers. In addition, Glycodelin serum concentrations were analyzed in patients suffering from benign (n = 73) or malignant (n = 38) ovarian neoplasias.ResultsGlycodelin A was found to be an independent prognostic marker for poor prognosis in advanced ovarian cancer patients. GdA staining correlated with gonadotropin receptor (FSHR and LHCGR) and with hCG expression. Gd expression showed a positive correlation with a tumour-associated epitope of mucin 1 (TA-MUC1). Further, compared to ovarian cancer, serum Gd was increased in patients with benign ovarian tumors.ConclusionGlycodelin A might be related to tumor aggressiveness and poor clinical outcome in advanced epithelial ovarian cancer. Glycodelin serum levels found in patients suffering from benign ovarian tumors, might contribute to a more global attenuation during progression of these precursor lesions.

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Section: Discussionmentioning

confidence: 99%

Glycodelin A is a prognostic marker to predict poor outcome in advanced stage ovarian cancer patients

et al. 2012

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“…Generally, traditional immunohistochemical markers used for the distinction of epithelial, stromal and granulosa ovarian tumor, such as CK, vimentin and inhibin A can be useful, and sometimes, they may show overlapping patterns of expression [23,24]. For example, vimentin could be immunostained in mucinous tumor, serous tumor, and low-grade endometrioid tumor [25-27]; inhibin A could be strongly expressed in epithelial ovarian cancer and other types of ovarian malignancies [25,26].…”

Section: Discussionmentioning

confidence: 99%

Enhanced efficacy and specificity of epithelial ovarian carcinogenesis by embedding a DMBA-coated cloth strip in the ovary of rat

et al. 2012

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BackgroundOvarian cancer is predominant of epithelial cell origin and often present at an advanced stage with poor prognosis. Most animal models of ovarian carcinoma yield thecal/granulose cell tumors, rather than adenocarcinomas. The best reported induction rate of adenocarcinoma in rats is 10-45% by an ovarian implantation of 7, 12-dimethylbenz[a]anthracene (DMBA) coated silk suture. We provided an improved procedure to construct the model by the ovarian implantation of DMBA-coated cloth strip.MethodsA sterile suture (as S group) or a piece of cloth strip (as CS group) was soaked in DMBA before ovarian implantation in Wistar rats. Tumor size, incidence rate and pathological type were analyzed.ResultsOvarian tumors in rats of CS group were first noted at 16 wk post implantation and reached a cumulative incidence of 75% (96/128) at 32 wk, while the tumor incidence rate in S group at 32 wk was only 46.25% (37/80). The tumor size in CS group (3.63 ± 0.89 cm) was larger than that of S group (2.44 ± 1.89 cm) (P < 0.05). In CS group, there were only two types of tumor formed: adenocarcinoma (90/96) and sarcoma (6/96). While in S group, there were different types, including adenocarcinoma (21/37), squamous carcinoma (3/37), granulosa cell tumor (3/37), sarcoma (4/37), undifferentiated carcinoma with no adeno character (2/37), benign ovarian tumor (2/37), and malignant teratoma (1/37).ConclusionThe model in our study yields much higher incidence and specificity of epithelial derived tumors and showed histological similarities to human ovarian cancers, which would be more suitable for therapeutic research.

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“…Gd A is primarily produced in endometrial and decidual tissues and secreted into the amniotic Xuid. With the help of a novel Gd antibody, Jeschke et al (2009) studied Gd localization in serous, mucinous, endometrioid and clear cell ovarian tumors. A Gd isoform was also puriWed from ascites Xuid of ovarian cancer patients but showed signiWcant diVerences in sialyl Lewis-type oligosaccharides as compared to Gd A. Interestingly, this Gd isoform inhibited IL-2-induced proliferation of peripheral blood leukocytes.…”

Section: Cancermentioning

confidence: 99%

Extending the knowledge in histochemistry and cell biology

Heupel

Drenckhahn

2009

Histochem Cell Biol

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Central to modern Histochemistry and Cell Biology stands the need for visualization of cellular and molecular processes. In the past several years, a variety of techniques has been achieved bridging traditional light microscopy, fluorescence microscopy and electron microscopy with powerful software-based post-processing and computer modeling. Researchers now have various tools available to investigate problems of interest from bird's- up to worm's-eye of view, focusing on tissues, cells, proteins or finally single molecules. Applications of new approaches in combination with well-established traditional techniques of mRNA, DNA or protein analysis have led to enlightening and prudent studies which have paved the way toward a better understanding of not only physiological but also pathological processes in the field of cell biology. This review is intended to summarize articles standing for the progress made in "histo-biochemical" techniques and their manifold applications.

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Immunohistochemistry, glycosylation and immunosuppression of glycodelin in human ovarian cancer

Cited by 17 publications

References 56 publications

Glycodelin A is a prognostic marker to predict poor outcome in advanced stage ovarian cancer patients

Glycodelin A is a prognostic marker to predict poor outcome in advanced stage ovarian cancer patients

Enhanced efficacy and specificity of epithelial ovarian carcinogenesis by embedding a DMBA-coated cloth strip in the ovary of rat

Extending the knowledge in histochemistry and cell biology

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