Immunohistochemistry and RT‐PCR as ancillary tools in the diagnosis of acquired oral syphilis
Maria Eduarda Zeraik Barreto,
Ciro Dantas Soares,
Maíra Medeiros Pacheco de Andrade
et al.
Abstract:ObjectiveTo investigate Treponema pallidum detection using immunohistochemistry (IHC) and reverse transcription‐polymerase chain reaction (RT‐PCR) assays in acquired oral syphilis (AOS).Materials and MethodsThirty‐seven paraffin‐embedded tissue specimens of AOS (32 secondary and five primary) were analyzed, integrating double‐positive serological results with clinicodemographic and histopathological data. T. pallidum presence was semiquantitatively assessed by IHC, while RT‐PCR targeted T. pallidum DNA. Sensit… Show more
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