Immunohistochemical visualization of a metabotropic glutamate receptor

Tj, Görcs; Penke, Botond; Böti, Z; Katarova, Zoya; Hámori, J.

doi:10.1097/00001756-199303000-00014

Cited by 97 publications

(24 citation statements)

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“…However, cerebellar Purkinje cells expressing large amounts of mGluR1 mRNA as well as mGluR1a protein Baude et al, 1993;Görcs et al, 1993;Grandes et al, 1994) are not labeled by the present mGluR5 antiserum. Thus under the conditions of the immunocytochemical stainings, there is no evidence for such cross reactivity.…”

Section: Characterization Of the Mglur5 Antiserummentioning

confidence: 62%

“…Both mGluR1 and mGluR5 are expressed in the cerebellar cortex with distinct cellular distribution patterns. Purkinje cells express high levels of mGluR1 mRNA , and immunohistochemical studies revealed that the mGluR1 protein is localized in spiny protrusions of the Purkinje cells postsynaptic to parallel fiber terminals (Baude et al, 1993;Görcs et al, 1993). This localization is consistent with the functional significance of mGluR1 for the induction of the long term depression (LTD) of the parallel fiber-Purkinje cell synaptic transmission (Conquet et al, 1994).…”

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confidence: 54%

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Light and electron microscopic demonstration of mGluR5 metabotropic glutamate receptor immunoreactive neuronal elements in the rat cerebellar cortex

et al. 1997

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The cellular and subcellular localization of the mGluR5 metabotropic glutamate receptor subtype was studied in the rat cerebellar cortex, by using the preembedding immunoperoxidase and immunogold techniques. Light microscopic observations revealed an abundant, intense labeling of neurons in the granular layer as well as in the molecular layer. Lugaro and Golgi cells exhibited an intense mGluR5 immunoreactivity, while only a fraction of the neurons in the molecular layer were found to be mGluR5 immunopositive. In addition to a dense plexus of immunoreactive dendrites in the molecular layer of the cerebellar cortex, the mGluR5 immunopositive Golgi cell dendrites resembling axons at the light microscopic level were also labeled in the granular layer. At the ultrastructural level, mGluR5 immunoreactivity was present in neuronal elements postsynaptic to axon terminals of different morphology. By using a pre-embedding immunogold method, it was found that mGluR5 immunoreactivity is accumulated at the plasma membranes extrasynaptically as well as at the periphery of the postsynaptic specializations, mainly of the parallel fiber synaptic contacts. These findings provide morphological evidence that mGluR5 is expressed by a population of neurons in the cerebellar cortex and can synaptically be activated via the parallel fiber system.

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Section: Characterization Of the Mglur5 Antiserummentioning

confidence: 62%

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confidence: 54%

Light and electron microscopic demonstration of mGluR5 metabotropic glutamate receptor immunoreactive neuronal elements in the rat cerebellar cortex

et al. 1997

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“…Previous studies have shown that every SOM-expressing neuron in the rat hippocampus coexpresses the "a" spliced form of the metabotropic glutamate receptor 1 (mGluR1a) with a 1:1 correlation (Martin et al, 1992;Baude et al, 1993;Görcs et al, 1993;Blasco-Ibáñez and Freund, 1995;Kerner et al, 1997). Fluorescence immunohistochemistry was thus performed for mGluR1a.…”

Section: Hippocampal Egfp-expressing Cells Are Somatostatinexpressingmentioning

confidence: 99%

Novel Hippocampal Interneuronal Subtypes Identified Using Transgenic Mice That Express Green Fluorescent Protein in GABAergic Interneurons

Oliva¹,

et al. 2000

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The chief inhibitory neurons of the mammalian brain, GABAergic neurons, are comprised of a myriad of diverse neuronal subtypes. To facilitate the study of these neurons, transgenic mice were generated that express enhanced green fluorescent protein (EGFP) in subpopulations of GABAergic neurons. In one of the resulting transgenic lines, called GIN (GFP-expressing Inhibitory Neurons), EGFP was found to be expressed in a subpopulation of somatostatin-containing GABAergic interneurons in the hippocampus and neocortex. In both live and fixed brain preparations from these mice, detailed microanatomical features of EGFP-expressing interneurons were readily observed. In stratum oriens of the hippocampus, EGFPexpressing interneurons were comprised almost exclusively of oriens/alveus interneurons with lacunosum-moleculare axon arborization (O-LM cells). In the neocortex, the somata of EGFP-expressing interneurons were largely restricted to layers II-IV and upper layer V.In hippocampal area CA1, two previously uncharacterized subtypes of interneurons were identified using the GIN mice: stratum pyramidale interneurons with lacunosum-moleculare axon arborization (P-LM cells) and stratum radiatum interneurons with lacunosum-moleculare axon arborization (R-LM cells). These newly identified interneuronal subtypes appeared to be closely related to O-LM cell, as they selectively innervate stratum lacunosum-moleculare. Whole-cell patch-clamp recordings revealed that these cells were fast-spiking and showed virtually no spike frequency accommodation. The microanatomical features of these cells suggest that they function primarily as "input-biasing" neurons, in that synaptic volleys in stratum radiatum would lead to their activation, which in turn would result in selective suppression of excitatory input from the entorhinal cortex onto CA1 pyramidal cells.

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“…The sections were washed overnight in PB at 4°C, cryoprotected with 10, 20, and 30% sucrose in PB, frozen and thawed twice in liquid N 2 , washed several times in PB, and incubated with monoclonal mGluR1a antibodies, previously characterised (Kiss et al, 1996), either using the cell culture supernatants diluted 1:1-1:20 or the purified IgG at a concentration of 0.01-0.1 µg/ml. The immunohistochemical protocol was as described previously (Görcs et al, 1993;Grandes et al, 1994;Kiss et al, 1996); using 3,3,-diamino-benzidine (DAB) as chromogen (0,0125%, and 0,005% hydrogen peroxide; time of incubation 5-15 min, at room temperature). After the incubation, 3-3 sections (from each animal) were osmicated (1% OsO 4 in PB for 1 hr), dehydrated, and flat-embedded in Durcupan ACM (Fluka, Buchs, Switzerland).…”

Section: Materials and Methods Animals: Histological Processingmentioning

confidence: 99%

“…In fact, mGluR1a transcripts (Masu et al, 1991;Shigemoto et al, 1992) and mGluR1a immunoreactivity (Martin et al, 1992;Baude et al, 1993;Görcs et al, 1993;Ryo et al, 1993;Grandes et al, 1994;Nusser et al, 1994;Takács et al, , 1997 are present at high density in cerebellum, the highest density being observed in Purkinje cells (PCs) and especially in their dendritic spines, but the receptor is also present in some interneurons of the molecular layer and in some Golgi neurons in the granular layer.…”

Section: Introductionmentioning

confidence: 99%

Metabotrop glutamate receptor type 1a expressing unipolar brush cells in the cerebellar cortex of different species: A comparative quantitative study

et al. 1999

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Morphology, distribution and number of unipolar brush cells (UBCs) was studied in the cerebellar vermal lobules I-X of the chicken, rat, guinea pig, cat, and monkey using monoclonal mGluR1a antibody as a marker to visualise these recently described nerve cells (Mugnaini and Floris [1994] J. Comp. Neurol. 339:174-180; Mugnaini et al. [1994] Synapse 16:284-311). The morphological appearance of mGluR1a immunopositive UBCs is similar in all species investigated: they are small cells, having a single, relatively short and thick dendrite, terminating in brush-like dendrioles. Although this, probably excitatory, cell type can be found all over the cerebellar cortex, highest density of UBCs can be seen in the vermal cortex. The present study, therefore, was focused on the quantitative morphology and distribution of UBCs in the 10 lobules of the vermis. Calculating the number of UBCs/l Purkinje cell (PC), we have found differences in this value (average in vermal lobules I-X) from 1.04 in rat, 1.10 in chicken, 1.16 in guinea pig, 2.27 in monkey, and up to 2.44 in cat. The highest density of UBCs was observed in lobules I, IX, and X, whereas the lowest number of UBCs/l PC was found in lobules IV-VI (in the mammals) and in lobules VII-VIII (in the chicken). In mammals, particularly the monkey and cat, an increased presence of UBCs was observed in vermal sub-lobules VIc-VIIb,c, a region defined as the oculomotor vermis because of its role in the control of saccadic eye movement. There is also a basic difference between chicken and mammals in the distribution of UBCs within the lobules: in mammals, the lowest density of these nerve cells was found in the peripheral portion of the lobules, near to the pia, while in the chicken, in contrast, the density of UBCs was the highest subpially with fewer UBCs located in the deepest curvature of the lobules. Finally, the functional significance of the differences in the density and in the distribution pattern of UBCs in the cerebellar vermis between the phylogenetically different species investigated is briefly discussed.

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Immunohistochemical visualization of a metabotropic glutamate receptor

Cited by 97 publications

References 0 publications

Light and electron microscopic demonstration of mGluR5 metabotropic glutamate receptor immunoreactive neuronal elements in the rat cerebellar cortex

Light and electron microscopic demonstration of mGluR5 metabotropic glutamate receptor immunoreactive neuronal elements in the rat cerebellar cortex

Novel Hippocampal Interneuronal Subtypes Identified Using Transgenic Mice That Express Green Fluorescent Protein in GABAergic Interneurons

Metabotrop glutamate receptor type 1a expressing unipolar brush cells in the cerebellar cortex of different species: A comparative quantitative study

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