Immunohistochemical survey of the distribution of epidermal melanoblasts and melanocytes during the development of UVB-induced pigmented spots

Furuya, Rikako; Yoshida, Yuzo; Moro, Osamu; Tsunenaga, Makoto; Aoki, Hideaki; Kishimoto, Jiro; Ifuku, Ohji; Hirobe, Tomohisa

doi:10.1016/j.jdermsci.2009.03.012

Cited by 11 publications

(7 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The markers were expressed in the sequence of c-kit, tyrosinase-related proteins 1 and 2, and melanin, resembling normal melanocyte differentiation. 18,19 Whatever the mechanism involved in our patients, the present findings, combined with earlier laboratory data, 18,19 imply that UVB radiation contributes to the development of solar lentigines. Lastly, it is noteworthy that in two of our 10 patients, IGH-like lesions, characterized by small, non-scaly, white macules with a relative decrease in melanocyte number on histology, developed concomitantly with the NB-UVB-induced lentigines.…”

Section: Discussionsupporting

confidence: 71%

NB‐UVB (311–312 nm)‐induced lentigines in patients with mycosis fungoides: a new adverse effect of phototherapy

Friedland¹,

David

Feinmesser

et al. 2011

Acad Dermatol Venereol

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 71%

NB‐UVB (311–312 nm)‐induced lentigines in patients with mycosis fungoides: a new adverse effect of phototherapy

Friedland¹,

David

Feinmesser

et al. 2011

Acad Dermatol Venereol

View full text Add to dashboard Cite

show abstract

“…To address this issue, we conducted a study on the relationship between McSCs and epidermal pigmentation in F1 mice of HR-1 Â HR/ De. These mice possess melanin and epidermal melanocytes in dorsal skin and are a suitable animal model to investigate the molecular mechanism underlying UV-induced epidermal pigmentation (Furuya et al, 2009). We examined the changes in the number and localization of melanocytes during 4-week UVB exposure and found that UVB irradiation induced McSC differentiation into melanoblasts in hair follicles.…”

Section: Introductionmentioning

confidence: 99%

Wnt/β-Catenin and Kit Signaling Sequentially Regulate Melanocyte Stem Cell Differentiation in UVB-Induced Epidermal Pigmentation

Yamada

Hasegawa

Inoue³

et al. 2013

Journal of Investigative Dermatology

131

134

View full text Add to dashboard Cite

UV radiation is a well-known inducer of epidermal pigmentation that is utilized in therapy for vitiligo, one of the skin depigmentation disorders. Although it has been reported that melanocyte stem cells (McSCs) play essential roles in hair pigmentation, the relationship between McSCs and epidermal pigmentation remains unclear. Repetitive UVB irradiation on the dorsal skin of F1 mice of HR-1 × HR/De caused apparent epidermal pigmentation, and it was characterized by increase in the number of melanocytes. Interestingly, differentiation of McSCs into melanoblasts in hair follicles was followed by induction of epidermal melanocyte differentiation. Administration of a neutralizing antibody for Kit receptor that depletes resident melanoblasts could not suppress increased number of melanocytes. UVB irradiation also induced robust expression of Wnt7a as well as Kitl in epidermis, and β-catenin translocation into nucleus in McSCs. Intradermal injection of IWR-1 (inhibitor of Wnt response 1), a chemical inhibitor of β-catenin activation, and small interfering RNA (siRNA) against Wnt7a suppressed increase in the number of epidermal melanocytes. Taken altogether, it was demonstrated that Wnt7a triggered McSCs differentiation through β-catenin activation, and Kitl might induce following migration of melanoblasts to epidermis. These findings will help in developing therapeutic technologies for vitiligo and other pigmentary disorders.

show abstract

“…In the vertebrate epidermis, melanocytes are in close contact with surrounding keratinocytes via their dendritic processes. This close association allows melanocytes to accomplish their primary function of producing and delivering melanin to keratinocytes, thereby providing skin pigmentation and hair coloration (Furuya et al 2009). Hyperpigmentation results from an increase in melanin, and its treatment remains a challenge.…”

Section: Introductionmentioning

confidence: 99%

Primary culture of human face skin melanocytes for the study of hyperpigmentation

Tang

Jing

2013

Cytotechnology

View full text Add to dashboard Cite

Facial epidermal pigmentation and skin tumors can be caused by UV exposure and other physical and chemical irritations. In this report we describe the primary culture of melanocytes from human face skin. The ability to culture these melanocytes will enable their morphological and biological properties to be investigated. Skin specimens were obtained from patients who had undergone lower blepharoplasty procedures. Digestion with neutral protease and trypsin was used to obtain single cell suspensions of epidermal cells. The cells were cultured in M254 medium supplemented with human melanocyte growth solution. Cell morphology was observed using inverted microscopy. Melanocytes were positively identified using both L-DOPA staining and S-100 protein immunohistochemical staining. Immunofluorescence was used to confirm the expression of tyrosinase-related protein-1, a melanocyte-specific protein. The cellular ultrastructure of the melanocytes was observed by transmission electron microscopy. The cultured human melanocytes from face skin were multi-dendritic, and many mature melanosomes were observed. Therefore, using a specific culture medium, melanocytes from face skin can be successfully cultured and made available for further investigations.

show abstract

Immunohistochemical survey of the distribution of epidermal melanoblasts and melanocytes during the development of UVB-induced pigmented spots

Cited by 11 publications

References 47 publications

NB‐UVB (311–312 nm)‐induced lentigines in patients with mycosis fungoides: a new adverse effect of phototherapy

NB‐UVB (311–312 nm)‐induced lentigines in patients with mycosis fungoides: a new adverse effect of phototherapy

Wnt/β-Catenin and Kit Signaling Sequentially Regulate Melanocyte Stem Cell Differentiation in UVB-Induced Epidermal Pigmentation

Primary culture of human face skin melanocytes for the study of hyperpigmentation

Contact Info

Product

Resources

About