Immunohistochemical study on VEGF expression in endometrial carcinoma – comparison with p53 expression, angiogenesis, and tumor histologic grade

Abstract

VEGF expression was not a single specific indicator of angiogenesis in endometrial carcinoma, whereas it was significantly correlated with p53 expression.

PTEN immunohistochemical expression is suppressed in G1 endometrioid adenocarcinoma of the uterine corpus

“…The staining methods were described elsewhere (Fujisawa et al 2001;Kato et al 2003;Kyushima et al 2002;Watanabe et al 2002). In brief, the antibodies used were those for Ki-67 (rabbit polyclonal, 1:50, Dako, Kyoto, Japan), cdk2 (rabbit polyclonal, 1:2000, Santacruz, Calif., USA), cyclin A (clone 6E6, 1:100, Novocastra), cyclin D1 (clone DCS-6, 1:80, Oncogene, Mass., USA), cyclin E (clone 13A3, 1:40, Novocastra), p27 (clone 1B4, 1:200, Novocastra) and p53 (clone DO-7, 1:80, Novocastra).…”

High expression of skp2 correlates with poor prognosis in endometrial endometrioid adenocarcinoma

“…Positive staining was evaluated by counting approximately 1,000 cells in high-power fields and was expressed as the labeling index (LI), which was regarded as the percentage of positive nuclei. The result of skp2 expression was compared with those of p27, Ki-67, cdk2, cyclin A, cyclin D1, cyclin E, p53 and PTEN, which had been analyzed in our laboratory Akaboshi et al 2001;Kyushima et al 2002;Nishimura et al 2004;Kato et al 2003;Fujisawa et al 2001;Kimura et al 2004). …”

VEGF and Its Receptors (flt-1 and KDR/flk-1) as Prognostic Indicators in Endometrial Carcinoma

“…VEGF expression did not correlate with histological grade or the number of microvessels in the tumour area. They concluded that further follow-up study was mandatory to clarify the role of VEGF expression as a prognostic factor in endometrial carcinoma [12] .…”

“…Fine et al [11] showed in a small patient series that VEGF and its receptors fl t-1 and KDR/fl k-1 did not correlate with the incidence of metastases, recurrence and survival. Fujisawa et al [12] conducted a study comparing VEGF expression, angiogenesis, p53 expression and tumour histological grade. VEGF expression did not correlate with histological grade or the number of microvessels in the tumour area.…”