Immunohistochemical Study on the Initiation of Acid Phosphatase Secretion in the Human Prostate Cytochemistry and Biochemistry of Acid Phosphatases IV

Abstract

Acid phosphatase was purified from human prostatic tissue and from seminal plasma. Antisera to antigens from both sources were raised in rabbits. These antisera have been used for immunohistochemical localization of the respective antigens in the prostates of neonatal, infantile, prepubertal, and adult individuals. Immunoreactivity of the prostatic epithelium with the seminal fluid-derived antigen developed progressively in pubertal specimens with increasing age. It was not present in fetal and infantile organs. Antiserum prepared from human prostatic tissue-derived acid phosphatase gave a positive immunoreaction both with stroma and epithelium of the pre- and postpubertal glands. The results give evidence for a clear cut androgen-dependence in the appearance of the acid phosphatase present in semen, which therefore has been identified as secretory. The second antigen is nonsecretory, tissue-bound, nonandrogen dependent, and shares antigenic determinants with leukocyte-derived acid phosphatase.

Distribution and subcellular localization of a lysosome-associated protein in human genital organs

“…Similar findings have been reported by other authors (Abrahamsson 1988). The present finding of labeling of the vacuoles with the lamp antibody is clearly in favor of the previously suggested relationship between prostatic secretory vacuoles and lysosomes (Aumüller 1979).…”

“…After clotting, the antiserum was obtained by centrifugation and was purified by absorption with human seminal secretory proteins adsorbed to nitrocellulose paper strips. A polyclonal rabbit antibody, directed against the secretory prostatic acid phosphatase isoenzyme was obtained from Prof. Seitz from our department (Aumüller et al 1983;Seitz and Aumüller 1985). Secondary antimouse or anti-rabbit IgG antibodies, either FITC or CY3-labeled, unlabeled anti-rabbit IgG and soluble peroxidase-anti-peroxidase complex (PAP) were purchased from Dianova (Hamburg, Germany).…”

“…The close morphological relationship of secretory vacuoles in the human prostate with lysosomes (Aumüller 1979) prompted us to study the ultrastructural localization of lamp 2 in the human prostate. As the latter poses a number of questions regarding its mode of secretion (viz.…”

Cytochemistry and biochemistry of acid phosphatases VI: Immunoelectron microscopic studies on human prostatic and leukocytic acid phosphatases

“…The respective isoenzymes, which are either secretory (antigen I) or lysosomal (antigen II), have been purified and used for antibody production. As has been shown earlier [2], both antibodies have a markedly different binding pattern in the maturing prostate. It was demonstrated that immunoreactivity against secretory acid phosphatase (isoenzyme 2a) develops progressively within prostatic secretory cells during puberty, while the lysosomal isoenzyme (isoenzyme 3) is already present in the fetal and prepubertal gland.…”

Exploring the Origins of the Normal Prostate and Prostate Cancer Stem Cell

“…Intermediate forms between basal and luminal cells were not observed in the developing human prostate [14]. By 14 years of age, secretory PAP and PSA immunoreactivity are detected in prostatic epithelium and PAS-positive cells gradually become less and are absent in the normal adult prostate [4,17].…”