Immunohistochemical study of the gastrointestinal endocrine cells in the Korean aucha perch

Abstract

The regional distribution and relative frequency of neurohormonal peptides-producing cells were demonstrated in the gastrointestinal (GI) tract of the Korean aucha perch Coreoperca herzi, using 10 types of specific antisera raised against mammalian regulatory peptides. The GI tract was divided into four portions: stomach, gastro-intestinal junction, and small and large intestine. Most of the immunoreactive (IR) cells were in the mucosal epithelium and they were generally spindle shaped with a long cytoplasmic process. In addition, ovoid cells were found in the gastric regions. Serotonin-, somatostatin-, glucagon-, cholecystokinin-8 (CCK-8)-and pancreatic polypeptide (PP)-IR cells were observed with various relative frequencies. No chromogranin A-, secretin-, vasoactive intestinal peptide-, substance P-or bombesin-IR cells, however, were found. Serotonin-IR cells occurred throughout the GI tract and were the most numerous. Somatostatin-IR cells were restricted to the stomach and gastro-intestinal junction in numerous and moderate frequencies, respectively, but small numbers of glucagon-IR cells were restricted to the small intestine. Numerous CCK-8-IR cells were found in the small intestine but variable numbers of PP-IR cells occurred throughout the GI tract except for the large intestine. In general the distribution and relative frequency of these IR cells correspond well to previous reports in teleosts but there are some difference in this species. # 2004 The Fisheries Society of the British Isles

Immunohistochemical study of the digestive tract ofOligosarcus hepsetus

“…It is known that in fish, serotonin promotes gastrointestinal motility [51] and blood flow, in the latter case by triggering vasoconstriction [52,53] . In O. hepsetus, 5-HT-IR cells were observed only in the regions of the stomach, both in the lining epithelium and the glandular epithelium, as also observed in C. herzi by Lee et al [44] . As was described for the fish species Oncorhynchus mykiss [54] , Salmo trutta [55] , Odontesthes bonariensis [56] and Rhamdia quelen [57] , in O. hepsetus we observed CCK-IR cells in the intestine; in this case only in the posterior part, while in O. bonariensis [56] these cells were observed throughout the gut, but with greater concentration in the hindgut.…”

“…Although we did not visualize this hormone in the intestinal parts of the thin dogfish, it was reported in the gut of the spiny dogfish Squalus acanthias [41] , Oncorhynchus mykiss [42] , P. fulvidraco, M. albus, S. chuatsi [36] , Zacco platypus [43] and Coreoperca herzi (C. herzi) [44] . [44] , amphibians [45] , reptiles [46] , birds [47] and mammals [48,49] . Researchers state that all vertebrates have this type of endocrine cell in the DT, assuming that these cells' location is based on the evolution of these higher life forms [50] .…”

An immunohistochemical study on endocrine cells in the neuroendocrine system of the digestive tract of milkfish Chanos chanos (Forsskal, 1775)

“…Several regulatory hormones and peptides produced by the endocrine cells also have been reported in digest tract of fish (Pan, Fang & Huang 2000). It has been reported that the characteristics of structure and distribution of endocrine cells by using immunohistochemical techniques in several studies (Bosi, Di Giancamillo, Arrighi & Domeneghini 2004;Lee, Ku, Park & Lee 2004). It has been reported that the characteristics of structure and distribution of endocrine cells by using immunohistochemical techniques in several studies (Bosi, Di Giancamillo, Arrighi & Domeneghini 2004;Lee, Ku, Park & Lee 2004).…”

Gut development in Perca fluviatilis: a micro-anatomical study

“…In P. fluviatilis larvae, 5-HT it the first neuroendocrine factor to regulate epithelial secretion and muscle activity in the gut. (Lee et al 2004). At 20 dph, several endocrine cells containing a NPY-like molecule were observed in the intestinal epithelium of the larvae.…”