Immunohistochemical Study of Langerhans Cells in Skin Tumors

Abstract

The behavior of Langerhans cells in skin tumors was investigated by immunohistochemical techniques using OKT6. OKT6-positive cells were numerous in squamous cell carcinomas, seborrheic keratoses and keratoacanthomas. They were rare in basal cell carcinomas, Bowen’s disease, eccrine poromas, extramammary Paget’s disease and warts. In solar keratosis, the number of OKT6-positive cells was almost equal or slightly larger compared to the normal epidermis. These results indicate that the density of Langerhans cells may not correlate with the degree of malignancy but, to a certain extent, with the nature of the membrane of tumor cells occurring e.g. during keratinization.

Inverse modulation of intraepithelial Langerhans' cells and stromal macrophage/dendrocyte populations in human papillomavirus-associated squamous intraepithelial lesions of the cervix

“…We used a panel of antibodies useful in the identification of Langerhans' cells (anti-S100), mobile connective tissue histiocytes (anti-L1, CD68) and resident dendrocytes (anti-factor XIII). The antiserum for S100 protein has been shown to be a reliable immunohistochemical marker for Langerhans' cells [72,74]. The L1 antigen is a myelomonocytic antigen detected by the monoclonal antibody Mac 387 [11,23].…”

Langerhans' Cells in Skin Tumors

“…As in other investiga¬ tions,921"23 samples of the skin right next to the tumor were used as a control group. The reliability of this is ques¬ tionable as it has been demonstrated that the distribu¬ tion of LCs in this area is somewhat irregular.1524 23 Stene and Cochran9 employed a technique similar to that used in this investigation. They found a reduced number of LCs above the tumor in two cases of MM.…”