Abstract:Immunohistochemical distribution of [D-Ala2]deltorphin I (DADTI), a highly selective ligand for ƒÂ opioid receptors, was investigated in rat retina by using a specific antiserum. The antiserum against DADTI was raised in a rabbit by immunizing with a synthetic peptide (Tyr-D-Ala-Phe-Asp-Val-Val-Gly•ENH2) conjugated to poly-L-glutamate with water soluble carbodiimide. When examined by immunospot assay, the major epitope of the antiserum appeared to be in the C-terminal region containing Asp-Val-Val-Glu•ENH2, th… Show more
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