Immunohistochemical study of cyclooxygenase-2 in skin tumors

Amirnia, Mehdi; Babaie-ghazani, Arash; Fakhrjou, Ashraf; Khodaeiani, Effat; Alikhah, Hossein; Naghavi-Behzad, Mohammad; Zarrintan, Armin

doi:10.3109/09546634.2012.674191

Cited by 23 publications

(14 citation statements)

References 34 publications

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“…To evaluate the effect of resveratrol on p53 and proliferating cell nuclear antigen (PCNA) proteins, cells were processed by the immunocytochemistry technique using monoclonal antibodies and the EnVision Dakocytomation system (EnVision ® + system-HRP AEC, K4004, DAKO), as previously described by Amirnia et al (2014) with some modifications. Cells were seeded at a density of 2 × 10 4 cells/well on chamber slides.…”

Section: Immunocytochemistrymentioning

confidence: 99%

Antiproliferative and toxicogenomic effects of resveratrol in bladder cancer cells with different TP53 status

Almeida

Guerra

Assis

et al. 2019

Environ and Mol Mutagen

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The antitumor activity of resveratrol, a polyphenolic compound found mainly in grapes, has been studied in several types of cancer. In bladder cancer, its antiproliferative effects have already been demonstrated; however, its mechanism of action is not completely understood. The aim of this study was to evaluate resveratrol antitumor activity (12.5, 25, 50, 100, 150, 200, and 250 μM) and its possible mechanisms of action in bladder tumor cells with different TP53 gene status (RT4, grade 1, TP53 wild type; 5637‐grade 2 and T24‐grade 3, TP53 mutated). Cell proliferation, clonogenic survival, morphological changes, cell cycle progression, apoptosis rates, genotoxicity, global methylation, immunocytochemistry for p53 and PCNA and relative expression profiles of the AKT, mTOR, RASSF1A, HOXB3, SRC, PLK1, and DNMT1 were evaluated. Resveratrol decreased cell proliferation and induced DNA damage in all cell lines. Regarding the long‐term effects, resveratrol reduced the number of colonies in all cell lines; however, TP53 wild type cells were more resistant. Increased rates of apoptosis were found in the TP53 wild type cells and this was accompanied by AKT, mTOR, and SRC downregulation. In addition, the resveratrol antiproliferative effects in wild type TP53 cells were accompanied by modulation of the DNMT1 gene. In the TP53 mutated cells, cell cycle arrest at S phase with PLK1 downregulation was observed. Additionally, there was modulation of the HOXB3/RASSF1A pathway and nuclear PCNA reduction in the highest‐grade cells. In conclusion, resveratrol has antiproliferative activity in bladder tumor cells; however, the mechanisms of action are dependent on TP53 status. Environ. Mol. Mutagen., 60:740–751, 2019. © 2019 Wiley Periodicals, Inc.

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Section: Immunocytochemistrymentioning

confidence: 99%

Antiproliferative and toxicogenomic effects of resveratrol in bladder cancer cells with different TP53 status

Almeida

Guerra

Assis

et al. 2019

Environ and Mol Mutagen

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“…However, a major shift has recently occurred in this focus, by realization that tumor growth and its aggressiveness may be determined not only by the proliferative rate, but also by the rate of cell death (Amirnia et al, 2012). Assessment of apoptosis and individual components of the apoptosis pathway might therefore be relevant in determining the prognosis in an individual patient.…”

Section: Introductionmentioning

confidence: 99%

Bcl-2 Gene Expression in Human Breast Cancers in Iran

Rostamizadeh

Fakhrjou²,

Montazeri³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Background: Breast cancer is among the five most common cancers and ranks first among cancers diagnosed in Iranian women. Screening and treatment of this disease with molecular methods, especially regarding high incidences at early age and advanced stage, is essential. Several genes with altered expression have been identified by cDNA microarray studies in breast cancer, with the Bcl-2 gene indicated as a likely candidate. In this study, we studied Bcl-2 gene expression levels in parallel tumor and non-tumor breast tissues. Materials and Methods: Forty samples including 21 tumor, 16 non tumor (marginal) and 3 benign breast tissues which were all pathologically diagnosed, were subjected to RNA extraction and polyA RT-PCR with the expression level of Bcl-2 quantified using real-time PCR. Results: There is higher expression levels of the Bcl-2 gene in tumor samples compared with marginal samples, but not attaining significance(p>0.05). Bcl-2 expression in 14 (66.7%) of the cases of tumor samples and 9 (56.3%) cases of the marginal samples were positive. Comparison of the expression of the Bcl-2 gene in histological grade showed that a high expression of Bcl-2 was associated with a high histological grade (p<0.41). Conclusions: Our data suggests that dysregulated Bcl-2 gene expression is potentially involved in the pathogenesis of breast cancer. Using gene expression analysis may significantly improve our ability for screening cancer patients and will prove a powerful tool in the diagnosis and prognostic evaluation of the disease whilst aiding the cooperative group trials in the Bcl-2 based therapy project.

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“…Scientometrics explains science production process and efficiency of scientific researches (Osareh and Marefat 2006). Medical sciences and other related fields has attracted more attentions during recent years and more scientometric studies have been conducted in this regard in Iran (Malekzadeh et al, 2001;Foroughi and Kharazi 2006;Alijani and Karami 2010;Amirnia et al, 2012;Fakhrjou et al, 2013;Karami et al, 2013). Considering medical sciences, chronic diseases especially cancers have special importance because they are regarded as the main cause of mortality which follows cardiac diseases as main cause of mortality (Murray and Lopez 1997).…”

Section: Introductionmentioning

confidence: 99%

Knowledge Production Status of Iranian Researchers in the Gastric Cancer Area: Based on the Medline Database

Ghojazadeh

Naghavi-Behzad²,

Nasrolah-Zadeh³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Immunohistochemical study of cyclooxygenase-2 in skin tumors

Abstract: COX-2 expression was positive in skin tumors including malignant and pre-malignant skin lesions. This study strongly suggests that COX-2 can be one of the molecular targets in treating various skin tumors.

Cited by 23 publications

References 34 publications

Antiproliferative and toxicogenomic effects of resveratrol in bladder cancer cells with different TP53 status

Antiproliferative and toxicogenomic effects of resveratrol in bladder cancer cells with different TP53 status

Bcl-2 Gene Expression in Human Breast Cancers in Iran

Knowledge Production Status of Iranian Researchers in the Gastric Cancer Area: Based on the Medline Database

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