Immunohistochemical study of CD44s expression in oral squamous cell carcinoma-its correlation with prognostic parameters

Abstract

Background and Objectives:The study aims at the observation of the immunohistochemical expression of CD44s in Oral Squamous Cell Carcinoma (OSCC) and to correlate its expression with prognostic parameters.Materials and Methods:A total of 30 cases of OSCC, - 10 cases of each well differentiated (WD SCC), moderately differentiated (MD SCC) and poorly differentiated squamous cell carcinomas (PD SCC) were included in the study. The sections were subjected to immunohistochemical study using CD44s antigen marker. The degree of intensity and distribution of CD44s immunostaining was assessed and correlated with prognostic markers such as tumor stage (tumor size), tumor grade (Broder's histological grading), tumor site, tumor thickness (histological depth of invasion) and nodal status.Results:CD44s expression by tumor cells in OSCCs is statistically correlated with tumor grade i.e. Higher mean of CD44s immunoexpression was observed in WD SCC group (10.80 ± 3.97), followed by MD SCC group (5.90 ± 3.38) and PD SCC group showed least CD44s immunoexpression (3.70 ± 4.64). There was no statistical significance observed with respect to the other prognostic markers.Conclusion:Based on these observations it can be suggested that the decrease in expression of CD44s in OSCC cells may be due to the reduced cell-to-cell and cell-to-matrix adhesion, resulting in easy detachment from the rigid constitution. Low expression of CD44s in OSCC tissues may be an indicator of tumor invasion and high metastatic potential.

Differentially expressed full-length, fusion and novel isoforms transcripts-based signature of well-differentiated keratinized oral squamous cell carcinoma

“…Immunohistochemically, CD44 has been suggested to be a prognostic marker in OSCC and is correlated with metastasis [33]. Decreased expression in MD/PD/WD metastatic OSCC compared to WD OSCC further supports the above hypothesis.…”

Effect of Genistein and Oxaliplatin on Cancer Stem Cells in Oral Squamous Cell Carcinoma: An Experimental Study

“…After rinsing in phosphate buffered saline, the sections were incubated in secondary antiserum. They were then washed in PBS and incubated in avidine-biotine complex reagents and incubated in peroxidase reaction containing 0.01% H2O2 in PBS buffer (16).…”

Prognostic significance of elevated serum CD44 levels in patients with oral squamous cell carcinoma

“…In the present study, although significant correlation between serum/tumour CD44 concentration and recurrence and survival was detected, other clinicopathological parameters such as tumour stage, nodal metastasis, tumour grade and extranodal extension did not significantly correlate with CD44 concentration (Table ). Another study by Hema et al also did not find correlation between serum CD44 concentration and clinical parameters of patients with OSCC, although authors have not conducted clinical follow‐up in this study . The possible reasons for the discrepancy between laryngopharyngeal cancer and oral cancer could be‐(i) CD44 expression is site‐specific and different subsites of oral cavity need to be analyse separately and should be clearly mentioned in the study rather than just oral cavity.…”