Immunohistochemical Study of Aquaporin 1 and 2 in the Reproductive Tract of the Bactrian camel (Camelus bactrianus)

An, Fengli; Wang, Jianlin

doi:10.4067/s0717-95022016000400007

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…Prostatic secretion of prostate fluid is one of the important components of semen; it provide nutrients and a suitable environment for sperm. An and Wang [ 27 ] reported that AQP1 and AQP2 were localized in the prostate, showing that the water channel protein is closely related to the function and may influenced the transportation of sperm and nutrition. Our present finding showed that the immune reactivity of anti-AQP-7 antibody was intense in both compact and disseminated parts of the prostate gland during the rutting season.…”

Section: Discussionmentioning

confidence: 99%

Morphological and Molecular Investigations of Aquaporin-7 (AQP-7) in Male Camelus dromedarius Reproductive Organs

Althnaian

2023

Animals

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Aquaporins (AQP) are involved in bidirectional transfers of water and small solutes across cell membranes. They are present in all tissues. However, the expression of AQP-7 has not yet been demonstrated in the reproductive tract of the camelid Camelus dromedarius. The study presented here concerns the immunohistochemical evidence of aquaporin-7 (AQP-7) in different parts of the male genital tract of Camelus dromedarius. To check the immune reactivity levels of anti-AQP-7 antibody in the male genital tract of Camelus dromedarius, the testes (proximal part, distal part and rete testis), epididymis (head, body and tail), ductus deferens (initial, middle and ampullary part) and prostate gland (compact and disseminated part) were collected from 12 male camels during the rutting and non-rutting seasons and subjected to immunohistochemistry. The result showed that the highest level of AQP-7 mRNA expression was in the testis of rutting and non-rutting males compared to the ductus deferens, epididymis and prostate. In addition, the highest mRNA gene expression of AQP-7 was in rutting males compared to non-rutting males. AQP-7 mRNA expression was higher in the ret testis, the body of the epididymis, the ampullary part of the ductus deferens and the compact part of the prostate. The immune reactivity levels of AQP-7 in rutting males showed strong reactivity in the testis and prostate compared to the epididymis and ductus deferens. On the basis of the results, it can be concluded that the distribution of the AQP-7 transcript and protein varied among rutting and non-rutting seasons and that the physiological roles of AQP-7 in the transportation of lipids, energy and water should be considered the main challenge in the activity and establishment of male Camelus dromedarius fertility during the rutting and non-rutting seasons. Moreover, AQP-7 detection is critical in assessing regulation and screening for new modulators that can prompt the development of effective medication to enhance fertility during rutting and non-rutting seasons.

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Section: Discussionmentioning

confidence: 99%

Morphological and Molecular Investigations of Aquaporin-7 (AQP-7) in Male Camelus dromedarius Reproductive Organs

Althnaian

2023

Animals

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“…Many reports have demonstrated the expression of AQP proteins in the male reproductive system in different mammalian species such as humans ( Pastor-Soler et al, 2001 ), rodents ( Calamita et al, 2001a , 2001b ; Lu et al, 2008 ; Ford et al, 2014 ), Bactrian camel ( An & Wang 2016 ), stallion ( Klein et al, 2013 ), bat ( Oliveira et al, 2013 ), cat ( Arrighi & Aralla 2014 ), and dog ( Domeniconi et al, 2007 ). The expression and localization of AQP proteins in the male reproductive tissues are variable among the tested mammalian species ( Boj et al, 2015 ; Carrageta et al, 2020 ; Ribeiro et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Differential Expressions of Aquaporin Subtypes in the Adult Mouse Testis

Mohamed¹,

Im²,

Kim³

et al. 2022

Dev. Reprod.

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Many efforts have been made to study the expression of aquaporins (AQP) in the mammalian reproductive system, but there are not enough data available regarding their localized expression to fully understand their specific roles in male reproduction. The present study investigated the expression and localization patterns of different AQP subtypes in the adult mouse testes and testicular spermatozoa using an immunofluorescence assay. All the studied AQPs were expressed in the testes and revealed subtype-specific patterns in the intensity and localization depending on the cell types of the testes. AQP7 was the most abundant and intensive AQP subtype in the seminiferous tubules, expressing in Leydig cells and Sertoli cells as well as all stages of germ cells, especially the spermatids and testicular spermatozoa. The expression pattern of AQP3 was similar to that of AQP7, but with higher expression in the basal and lower adluminal compartments rather than the upper adluminalcompartment. AQP8 expression was limited to the spermatogonia and Leydig cells whereas AQP9 expression was exclusive to tails of the testicular spermatozoa and elongated spermatids. Taken together, the abundance and distribution of the AQPs across the different cell types in the testes indicating to their relavance in spermatogenesis, as well as in sperm maturation, transition, and function.

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Abundance of selected genes implicated in testicular functions in Camelus dromedarius with high and low epididymal semen quality

Rashad,

Ibrahim,

El-Sokary

et al. 2023

Biology of Reproduction

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Studying testicular genes´ expression may give key insights into precise regulation of its functions that influence epididymal sperm quality. The current study aimed to investigate the abundance of candidate genes involved in the regulation of testicular functions specially those regulate sperm function (PLA2G4D, SPP1 and CLUAP1), testicular steroidogenic function (ESR1 and AR), materials transport (AQP12B and LCN15) and defense mechanisms (DEFB110, GPX5, SOCS3 and IL6). Therefore, blood samples and testes with epididymis were collected from mature middle-aged (5–10 years) dromedary camels (n = 45) directly prior and after their slaughtering, respectively, during breeding season. Sera were evaluated for testosterone level and testicular biometry was measured with caliper. The epididymal tail semen was evaluated manually. Samples were distinguished based on testosterone level, testicular biometry, as well as epididymal semen features into high and low fertile groups. Total RNA was isolated from testicular tissues and genes expression was done using qRT-PCR. Results revealed that testosterone levels were significantly (P < 0.005) higher in camels with good semen quality than those of low quality. There was a significant (P < 0.0001) increase in testicular weight, length, width, thickness, and volume in high fertile than low fertile camels. PLA2G4D, SPP1, CLUAP1, ESR1, AR, AQP12B, LCN15, DEFB110, GPX5 and SOCS3 genes were up-regulated (P < 0.001), and IL6 gene was down-regulated (P < 0.01) in the testes of high fertile camels compared to the low fertile one. Thus, it could be concluded that examined genes might be valuable monitors of testicular functional status and fertility in dromedary camels.

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Immunohistochemical Study of Aquaporin 1 and 2 in the Reproductive Tract of the Bactrian camel (Camelus bactrianus)

Cited by 3 publications

References 19 publications

Morphological and Molecular Investigations of Aquaporin-7 (AQP-7) in Male Camelus dromedarius Reproductive Organs

Morphological and Molecular Investigations of Aquaporin-7 (AQP-7) in Male Camelus dromedarius Reproductive Organs

Differential Expressions of Aquaporin Subtypes in the Adult Mouse Testis

Abundance of selected genes implicated in testicular functions in Camelus dromedarius with high and low epididymal semen quality

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