Immunohistochemical Study of 3.BETA.-Hydroxysteroid Dehydrogenase /.DELTA.5-.DELTA.4 Isomerase in the Rat Cardiovascular System.

Oh, Seon Hee; Oh, Jae Min; Kim, Jeong Joong; Choi, Min Kyu; Park, Seung Taeck; Park, Ock Kyu; Chung, Yeun Tai

doi:10.1679/aohc.61.297

Cited by 4 publications

(8 citation statements)

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“…Results of our present study also suggest that local synthesis of progesterone may possibly contribute to its local action in the human aorta in which progesterone receptor has been reported to be present [13], especially in female aorta, as in the human nervous system. 3b-HSD immunoreactivity was reported to be present in VSMCs of rat abdominal aorta [1], which is consistent with results of our present study. These results all indicated that VSMCs in human aorta are not only targets of progesterone but also actively produce the steroid hormone in situ.…”

Section: Discussionsupporting

confidence: 93%

“…However, progesterone is also synthesized in non-reproductive tissues such as the central nervous system [1,[4][5][6]. Both steroidogenic endocrine glands and local synthesis are currently considered to contribute to the overall store of progesterone present in the brain and in human peripheral nerves.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the activity of this enzyme is demonstrated not only in classical steroidogenic tissues, but also in many peripheral tissues including human central nervous system [1,[4][5][6]. The presence of this enzyme in the cardiovascular system has been also documented in previously published reports [1,[6][7][8]. Especially, it has been demonstrated that there were relatively abundant levels of 3b-HSD activity in hearts of experimental animals [7,8].…”

mentioning

confidence: 82%

“…3b-HYDROXYSTEROID dehydrogenase (3b-HSD), which converts pregnenolone to progesterone, is wellknown to be essential for production of steroid hormones including progesterone, mineralcorticoids, glucocorticoids, androgens, and estrogens in classical steroidogenic tissues [1][2][3]. In addition, the activity of this enzyme is demonstrated not only in classical steroidogenic tissues, but also in many peripheral tissues including human central nervous system [1,[4][5][6].…”

mentioning

confidence: 99%

“…Especially, it has been demonstrated that there were relatively abundant levels of 3b-HSD activity in hearts of experimental animals [7,8]. Expression of 3b-HSD was also reported in human vascular smooth muscle cells (VSMCs) of artery and vein [1,7]. However, details regarding their expression in human cardiovascular system have not been examined.…”

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confidence: 99%

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3β-Hydroxysteroid Dehydrogenase in Human Aorta

et al. 2005

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Abstract. 3b-hydroxysteroid dehydrogenase (3b-HSD) is known to be involved in steroid production and/or metabolism and to be expressed in many tissues including adrenal cortex. Expression of this enzyme has also been elucidated in human cardiovascular system but its details remain largely unknown. Therefore, in this study, we examined the status of 3b-HSD in postmortem human aorta utilizing RT-PCR and immunohistochemical analysis. Both mRNAs and immunoreactivity for the enzyme were detected predominantly in female aorta, and in those with mild atherosclerotic changes. In addition, immunohistochemical study demonstrated that immunoreactivity for 3b-HSD was detected in vascular smooth muscle cells (VSMCs) of aorta. These findings all indicated that steroidogenesis via 3b-HSD may occur in VSMCs of human aorta, possibly related to gender differences and/or the degree of atherosclerosis.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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confidence: 82%

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confidence: 99%

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confidence: 99%

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3β-Hydroxysteroid Dehydrogenase in Human Aorta

et al. 2005

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Expression of 3 β-hydroxysteroid/dehydrogenase/Δ 5 -Δ 4 /isomerase in the tracheal cartilage of the rat

Oh¹,

Choi²,

Park³

et al. 2000

Anatomy and Embryology

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The enzyme complex 3beta-hydroxysteroid dehydrogenase isomerase/delta5-delta4 (3beta-HSD) is involved in the biosynthesis of all classes of active steroids. In this study, the presence of 3beta-HSD was defined in rat tracheal cartilage. The expression of the 3beta-HSD gene was examined by Northern blot analysis from 30-day-old rats. Western blot and immunohistochemical localization were also performed with antibodies raised against purified human placental 3beta-HSD to obtain further information on the expression of 3beta-HSD protein during fetal and postnatal periods of development in rat cartilage. Northern blot analysis using an oligonucleotide common to the 4 known 3beta-HSD isoforms showed 3beta-HSD mRNA corresponding to a transcript of 1.7 kb. Furthermore, a 42 KDa protein band was detected in the tracheal cartilage extracts by Western blot analysis. Immunostaining for 3beta-SD was observed in chondrocytes. The first expression was detected on the 17th day of fetal life by Western blot and immunohistochemistry. Immunoreactivity of 3beta-HSD showed a significant increase at 7 and 15 days after birth, and then remained unchanged through adulthood, in agreement with the data of the Western blot. Our results demonstrated the expression for 3beta-HSD in the tracheal cartilage at both the mRNA and protein levels during fetal life and postnatal development of the rat. These results suggest that 3beta-HSD may synthesize certain steroids which play major roles in differentiation and maintenance of function during development of rat cartilage.

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3β-HSD activates DHEA in the songbird brain

Schlinger¹,

Pradhan

Soma

2008

Neurochemistry International

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Dehydroepiandrosterone (DHEA) is an abundant circulating prohormone in humans, with a variety of reported actions on central and peripheral tissues. Despite its abundance, the functions of DHEA are relatively unknown because common animal models (laboratory rats and mice) have very low DHEA levels in the blood. Over the past decade, we have obtained considerable evidence from avian studies demonstrating that (1) DHEA is an important circulating prohormone in songbirds and (2) the enzyme 3beta-hydroxysteroid dehydrogenase/isomerase (3beta-HSD), responsible for converting DHEA into a more active androgen, is expressed at high levels in the songbird brain. Here, we first review biochemical and molecular studies demonstrating the widespread activity and expression of 3beta-HSD in the adult and developing songbird brain. Studies examining neural 3beta-HSD activity show effects of sex, stress, and season that are region-specific. Second, we review studies showing seasonal and stress-related changes in circulating DHEA in captive and wild songbird species. Third, we describe evidence that DHEA treatment can stimulate song behavior and the growth of neural circuits controlling song behavior. Importantly, brain 3beta-HSD and aromatase can work in concert to locally metabolize DHEA into active androgens and estrogens, which are critical for controlling behavior and robust adult neuroplasticity in songbirds. DHEA is likely secreted by the avian gonads and/or adrenals, as is the case in humans, but DHEA may also be synthesized de novo in the songbird brain from cholesterol or other precursors. Irrespective of its source, DHEA seems to be an important prohormone in songbirds, and 3beta-HSD is a key enzyme in the songbird brain.

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Immunohistochemical Study of 3.BETA.-Hydroxysteroid Dehydrogenase /.DELTA.5-.DELTA.4 Isomerase in the Rat Cardiovascular System.

Cited by 4 publications

References 25 publications

3β-Hydroxysteroid Dehydrogenase in Human Aorta

3β-Hydroxysteroid Dehydrogenase in Human Aorta

Expression of 3 β-hydroxysteroid/dehydrogenase/Δ 5 -Δ 4 /isomerase in the tracheal cartilage of the rat

3β-HSD activates DHEA in the songbird brain

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Immunohistochemical Study of 3.BETA.-Hydroxysteroid Dehydrogenase /.DELTA.5-.DELTA.4 Isomerase in the Rat Cardiovascular System.

Cited by 4 publications

References 25 publications

3&beta;-Hydroxysteroid Dehydrogenase in Human Aorta

3&beta;-Hydroxysteroid Dehydrogenase in Human Aorta

Expression of 3 β-hydroxysteroid/dehydrogenase/Δ 5 -Δ 4 /isomerase in the tracheal cartilage of the rat

3β-HSD activates DHEA in the songbird brain

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3β-Hydroxysteroid Dehydrogenase in Human Aorta

3β-Hydroxysteroid Dehydrogenase in Human Aorta