Immunohistochemical studies on the effect of capsaicin on spinal and medullary peptide and monoamine neurons using antisera to substance P, gastrin/CCK, somatostatin, VIP, enkephalin, neurotensin and 5-hydroxytryptamine

Jancsó, Gábor; Hökfelt, Tomas; Lundberg, Jan M.; Király, Elizabeth; Halasz, Nicholas A.; Nilsson, Göran; Terenius, Lars; Rehfeld, Jens F.; Steinbusch, Harry W.M.; Verhofstad, A.; Elde, Robert; Said, Sami I.; Brown, Marvin R.

doi:10.1007/bf01258524

Cited by 301 publications

(38 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Neonatal capsaicin treatment does not deplete SP from the striatum, nor does it alter the expression of the opioid peptides dynorphin and met 5 -enkephalin (40). With regard to monoaminergic systems, increased (41) or decreased 5-hydroxytryptamine levels (39), or no change at all (42), because of capsaicin treatment were reported in the very same year. The complete overlap between TH-and VR1-immunoreactive neurons in the nigra now finally identifies the vanilloid-sensitive nigral cells as monoaminergic.…”

Section: Discussionmentioning

confidence: 99%

Distribution of mRNA for vanilloid receptor subtype 1 (VR1), and VR1-like immunoreactivity, in the central nervous system of the rat and human

Mezey

Tóth

Cortright

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

628

284

View full text Add to dashboard Cite

The cloned vanilloid receptor VR1 has attracted recent attention as a molecular integrator of painful stimuli on primary sensory neurons. The existence of vanilloid-sensitive neurons in the brain is, however, controversial. In this study, we have used an antibody and a complementary RNA probe to explore the distribution of neurons that express VR1 in rat and in certain areas of human brain. In the rat, we observed VR1-expressing neurons throughout the whole neuroaxis, including all cortical areas (in layers 3 and 5), several members of the limbic system (e.g., hippocampus, central amygdala, and both medial and lateral habenula), striatum, hypothalamus, centromedian and paraventricular thalamic nuclei, substantia nigra, reticular formation, locus coeruleus, cerebellum, and inferior olive. VR1-immunopositive cells also were found in the third and fifth layers of human parietal cortex. Reverse transcription-PCR performed with rat VR1-specific primers verified the expression of VR1 mRNA in cortex, hippocampus, and hypothalamus. In the central nervous system, neonatal capsaicin treatment depleted VR1 mRNA from the spinal nucleus of the trigeminal nerve, but not from other areas such as the inferior olive. The finding that VR1 is expressed not only in primary sensory neurons but also in several brain nuclei is of great importance in that it places VRs in a much broader perspective than pain perception. VRs in the brain (and putative endogenous vanilloids) may be involved in the control of emotions, learning, and satiety, just to name a few exciting possibilities.

show abstract

Section: Discussionmentioning

confidence: 99%

Distribution of mRNA for vanilloid receptor subtype 1 (VR1), and VR1-like immunoreactivity, in the central nervous system of the rat and human

Mezey

Tóth

Cortright

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

628

284

View full text Add to dashboard Cite

show abstract

“…Similarly, the CGRP-LI-containing fi bers traveling in the longitudinal bundle of fibers ventral to the central canal may also be primary afferents of visceral origin. Jancso et al [25] reported that substance P immunoreactivity in this bundle is attenuated by the specific primary afferent toxin, capsaicin. Moreover, the ventral longitudinal bundle, which in the present study stained with CGRP-LI was labeled with HRP injected in the pelvic nerve [26], Since McKenna and Nadelhaft [ 12] did not trace any fibers to the ventral horns while we obser ved a substantial distribution of CGRP-LI fibers, these CGRP-LI fibers are probably not from the pudendal nerve.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Castration on Calcitonin Gene-Related Peptide in Spinal Motor Neurons

Popper¹,

Micevych²

1989

Neuroendocrinology

View full text Add to dashboard Cite

The spinal nucleus of the bulbocavernosus (SNB) is a sexually dimorphic nucleus of about 200 androgen-accumulating motor neurons in lumbar segments 5 and 6 of the rat spinal cord. In the present work we have used immunohistochemistry to investigate the change in calcitonin gene-related peptide-like immunoreactivity (CGRP-LI) in these motor neurons induced by long-term castration. CGRP-LI was found in the cell bodies of the SNB motor neurons and in thin varicose fibers coursing through the nucleus. In the intact animals 48.4 ± 3.9% (n = 8) of the SNB neurons were CGRP-LI positive while in the castrates this ratio increased to 80.0 ± 1.7% (n = 9, p < 0.001). Animals castrated for 4 weeks and then implanted with testosterone-containing Silastic capsules for an additional 4 weeks had the same percentage of CGRP-LI-containing SNB motor neurons as intact animals. These results demonstrate that a reduction in circulating testosterone levels increases the number of SNB motor neurons that express CGRP-LI implying that androgen stimulation inhibits the expression of CGRP-LI in SNB motor neurons.

show abstract

“…This is supported by the presence of chemo-sensitive afferent fibres in the rat's vagus nerves (Jancso & Kiraly, 1980), a population of which contain substance P (Gamse et al 1980). The possibility, however, that perineural capsaicin treatment may affect peptide-containing afferents other than those containing substance P can not be excluded, since it has been recently shown that neonatal capsaicin treatment affects somatostatin, cholecystokinin and vasoactive intestinal polypeptide-containing primary sensory neurones, as well (Jancso et al 1981). …”

Section: Chronic Experimentsmentioning

confidence: 84%

Effects of capsaicin applied perineurally to the vagus nerve on cardiovascular and respiratory functions in the cat.

Jancsó

Such

1983

The Journal of Physiology

View full text Add to dashboard Cite

SUMMARY1. The effects of capsaicin applied perineurally to the cervical vagus nerves have been studied on cardiovascular and respiratory functions in urethane anaesthetized cats.2. Application of capsaicin resulted in a moderate but significant decrease in the mean arterial blood pressure and in changes of the heart rate whose direction and magnitude depended on the initial cardiac frequency.3. Subsequent to these alterations, which may be attributed to a direct stimulation by capsaicin ofvagal afferents, a transient block ofimpulse propagation was observed.4. Three to five days after pre-treatment of the cervical vagus nerves with capsaicin, phenyldiguanidine and veratrine given intravenously invariably evoked bradycardia, hypotension and apnoea, while the reflex responses to intravenous injection of capsaicin and some of its pungent congeners were greatly reduced or even abolished.5. It is suggested that vagal afferent fibres mediating cardiovascular and respiratory chemo-reflexes are separated into chemo-specifically different populations. Perineural application of capsaicin may be a useful tool for elucidating the role of different populations of peptide-containing vagal afferent fibres in the regulation of cardiovascular and respiratory functions.

show abstract

Immunohistochemical studies on the effect of capsaicin on spinal and medullary peptide and monoamine neurons using antisera to substance P, gastrin/CCK, somatostatin, VIP, enkephalin, neurotensin and 5-hydroxytryptamine

Cited by 301 publications

References 65 publications

Distribution of mRNA for vanilloid receptor subtype 1 (VR1), and VR1-like immunoreactivity, in the central nervous system of the rat and human

Distribution of mRNA for vanilloid receptor subtype 1 (VR1), and VR1-like immunoreactivity, in the central nervous system of the rat and human

The Effect of Castration on Calcitonin Gene-Related Peptide in Spinal Motor Neurons

Effects of capsaicin applied perineurally to the vagus nerve on cardiovascular and respiratory functions in the cat.

Contact Info

Product

Resources

About