Immunohistochemical studies on proteoglycan expression in normal skin and chronic ulcers

Lundqvist, Katarina; Schmidtchen, Artur

doi:10.1046/j.1365-2133.2001.04009.x

Cited by 44 publications

(51 citation statements)

References 27 publications

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“…This staining pattern in skin and chondrocytes was similar to that previously reported (49,50) and in the present study served as a positive control for the antibodies. Immunostaining was negative in all experiments in which the primary antibodies to HSPGs and HS were replaced with the same concentrations of mouse ascitic fluid (for syndecans and glypican-1), normal goat IgG (for glypican-3), normal rabbit serum (for glypican-4), and normal mouse IgM (for HS) (data not shown).…”

Section: Cxcl8 Binding Site On Endothelial Syndecan-3 In Ra Synoviumsupporting

confidence: 91%

Induction of a CXCL8 binding site on endothelial syndecan‐3 in rheumatoid synovium

Patterson

Gardner

Shaw

et al. 2005

Arthritis & Rheumatism

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Section: Cxcl8 Binding Site On Endothelial Syndecan-3 In Ra Synoviumsupporting

confidence: 91%

Induction of a CXCL8 binding site on endothelial syndecan‐3 in rheumatoid synovium

Patterson

Gardner

Shaw

et al. 2005

Arthritis & Rheumatism

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“…Because HSPGs, such as syndecans and CD44, are expressed on the membranes of keratinocytes throughout much of the mucosa and epidermis (1,22,29), it is unclear how HS might participate in actively targeting virions to basal keratinocytes. Additionally, while HS appears to play at least a limited role in the infection of keratinocyte-derived cell lines by HPV-11 virions (12,33), this glycosaminoglycan showed no involvement in the infection of primary human foreskin keratinocytes by HPV-31b virions (30).…”

Section: Discussionmentioning

confidence: 99%

“…Multiple HSPGs including CD44, syndecans and glypicans are expressed on the membranes of keratinocytes throughout the epidermis and mucosa (22,29). ␣6 integrin expression is generally restricted to basal keratinocytes where this transmembrane protein pairs with ␤4 integrin and contributes to the nucleation of hemidesmosomes connecting the keratin cytoskeleton to the basement membrane (BM) (reviewed in reference 28).…”

mentioning

confidence: 99%

Keratinocyte-Secreted Laminin 5 Can Function as a Transient Receptor for Human Papillomaviruses by Binding Virions and Transferring Them to Adjacent Cells

Culp

Budgeon

Marinkovich

et al. 2006

J Virol

142

118

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Human papillomaviruses (HPVs) replicate only in the terminally differentiating epithelium of the skin and mucosa. While infection of basal keratinocytes is considered a requirement for permissive infection, it remains unclear whether virions can specifically target basal cells for adsorption and uptake following epithelial wounding. We present evidence that HPV binds specifically to laminin 5 (LN5), a component of the extracellular matrix (ECM) secreted by migrating and basal keratinocytes. HPV type 11 capsids colocalized with LN5 in the ECM secreted by vaginal keratinocytes. Binding of both virions and virus-like particles to purified LN5 and to the LN5-rich ECM secreted by cultured keratinocytes was effectively blocked by pretreatment with anti-LN5 antibodies. HPV capsid binding to human cervical mucosa sections included the basement membrane which contains LN5. Cultured keratinocytes expressing ␣6 integrin, a transmembrane protein known to bind LN5, were readily infected by virions preadsorbed to LN5-containing substrates, whereas mutant keratinocytes lacking ␣6 integrin were relatively resistant to infection via this route. These findings suggest a model of natural HPV infection in which proliferating keratinocytes expressing ␣6 integrin at the site of epithelial wounding might be targeted by virions adsorbed transiently to LN5 secreted by migrating keratinocytes.Human papillomavirus (HPV) particles have been shown to adsorb to the plasma membranes of cultured cells via membrane-associated heparan sulfate proteoglycans (HSPGs) (18,20,33) or ␣6 integrin (CD49f) (15,26). Multiple HSPGs including CD44, syndecans and glypicans are expressed on the membranes of keratinocytes throughout the epidermis and mucosa (22,29). ␣6 integrin expression is generally restricted to basal keratinocytes where this transmembrane protein pairs with ␤4 integrin and contributes to the nucleation of hemidesmosomes connecting the keratin cytoskeleton to the basement membrane (BM) (reviewed in reference 28). Results from experiments utilizing several in vitro infection models suggest that the importance of a particular receptor in HPV adsorption/infection may differ between cell lines and viral genotypes (12,30,33).In addition to binding directly to membrane-associated glycoproteins, we recently found that HPV capsids are also capable of binding a component of the extracellular matrix (ECM) secreted by keratinocytes, but not by nonkeratinocyte cell lines (12). Here we show evidence that this secreted HPV adsorption receptor is laminin 5 (LN5), an epithelial laminin secreted by migrating keratinocytes as they invade wounded epithelium (reviewed in reference 27). In the context of the ECM secreted by cultured keratinocytes, HPV virions can use LN5 as an extracellular "transreceptor" by transiently binding LN5 and subsequently transferring to entry receptors on adjacent cells. In another viral system, human immunodeficiency virus (HIV) is hypothesized to transiently bind DC-SIGN (CD209) on immature dendritic cells within the epithelium an...

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“…139,145 Moreover, deregulation of syndecan-1 and syndecan-4 have been detected in venous leg ulcers. 158 Fibronectin deficiency, most likely resulting from enhanced ECM degradation, has been reported to occur in chronic wounds where it may impair cell migration. 139,159,160 Moreover, fibronectin fragments (generated by fibronectin degradation) have been shown to modulate levels of MMPs and TIMPs in some models, 139,161 and have been reported to occur in chronic wound fluids.…”

Section: Future Directions Exploiting Integrins As Therapeutic Targetmentioning

confidence: 99%

Integrin Regulation of Epidermal Functions in Wounds

Longmate

DiPersio

2014

Advances in Wound Care

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Immunohistochemical studies on proteoglycan expression in normal skin and chronic ulcers

Abstract: The altered expression patterns of glypican and syndecan-1 and -4 in chronic ulcers reflect their possible roles during inflammation and cell proliferation. Hence, analysis of PG expression should be of interest in future studies on normal as well as defective wound healing.

Cited by 44 publications

References 27 publications

Induction of a CXCL8 binding site on endothelial syndecan‐3 in rheumatoid synovium

Induction of a CXCL8 binding site on endothelial syndecan‐3 in rheumatoid synovium

Keratinocyte-Secreted Laminin 5 Can Function as a Transient Receptor for Human Papillomaviruses by Binding Virions and Transferring Them to Adjacent Cells

Integrin Regulation of Epidermal Functions in Wounds

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