Immunohistochemical studies on collagen types in the uterine cervix in pregnant and nonpregnant states

Minamoto, Toshinari; Arai, Katsuhiko; Hirakawa, Shun; Nagai, Yutaka

doi:10.1016/0002-9378(87)90225-0

Cited by 75 publications

(43 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The extracellular matrix of the uterine cervix is composed mainly of collagen (types I and III), elastic fibres and proteoglycans (Minamoto et al 1987. During pregnancy the collagen network maintains the cervix as a rigid and closed structure in order to support the foetus in the uterus and prevent infections arising from the vagina.…”

Section: Discussionmentioning

confidence: 99%

Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

Varayoud¹,

Ramos²,

Bosquiazzo³

et al. 2004

Reproduction

View full text Add to dashboard Cite

During pregnancy, it is essential that sufficient nutrients are supplied by the vascular system to support the dramatic modifications of the rat uterine cervix. Angiogenesis refers to the growth of new blood vessels from pre-existing microcirculation and mast cells have been associated with this process. This study examined the modifications of the vascular compartment and the distribution of mast cells on cervical tissue during pregnancy. Using disodium cromoglycate as a mast cell stabilizer, we determined the effects of the mast cell degranulation on cervical angiogenesis. Mast cell distribution and their degranulation status were evaluated by immunohistochemistry. Endothelial cell proliferation was measured by bromodeoxyuridine incorporation. Vascular areas (absolute and relative) and maturation indices were assessed by quantitative immunohistochemistry of von Willebrand factor and a-smooth muscle actin respectively. Mast cells were predominantly observed during the first half of pregnancy in the perivascular zones. The values of bromodeoxyuridine incorporation, absolute vascular area and vascular maturation index exhibited a significant increase throughout pregnancy. All animals that received mast cell stabilizer showed more than 40% of non-degranulated mast cells. Treated rats exhibited a decrease in endothelial proliferation and in relative vascular area; in addition, a large proportion of mature blood vessels was observed, suggesting a diminished level of new vessel formation. The effects of the mast cell stabilizer were sustained beyond the end of treatment. This is the first report that brings evidence that mast cell degranulation could be a necessary process to contribute to the normal angiogenesis of the rat cervix during pregnancy. Further investigations are needed to elucidate the possible implications of abnormal vascular development of the uterine cervix on the physiological process of ripening and parturition.

show abstract

Section: Discussionmentioning

confidence: 99%

Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

Varayoud¹,

Ramos²,

Bosquiazzo³

et al. 2004

Reproduction

View full text Add to dashboard Cite

show abstract

“…Immunolocalization of collagen types I, III, IV, V, and VI was performed by the avidin-biotin complex method 26 (Vector Laboratories, Burlingame, Calif.) with protease digestion. 27 Polyclonal antibodies to collagen types I, III, IV, V, and VI were prepared by the methods described above. The working dilutions of the antibodies to collagen types I, III, and IV; collagen type V; and collagen type VI were 1:10,1:5, and 1:100, respectively.…”

Section: Immunohistochemical Examinationmentioning

confidence: 99%

Collagens in human atherosclerosis. Immunohistochemical analysis using collagen type-specific antibodies.

Katsuda

Okada

Minamoto

et al. 1992

Arterioscler Thromb

181

126

View full text Add to dashboard Cite

This study represents a systematic analysis of the distribution of collagen types in human atherosclerotic lesions. Formalin-fixed, paraffin-embedded aortic tissues of 40 lesions from 16 different individuals ranging in age from 1 month to 84 years were examined immunohistochemically using antibodies to type I, III, IV, V, and VI collagens. Preembedding immunoelectron microscopy was used to simultaneously localize type V and VI collagens within the lesions. Localization of type HI collagen was very similar to that of type L and type VI collagen appeared together with these two types of collagen in the thickened intlmas of all stages of the lesion. Type V collagen was not detected in either fatty streaks or the mild Intimal thickening of the aortas of children. With advancing age and lesion progression, the immunoreactivity with anti-type V collagen antibody became more intense. Type IV collagen was detected in the basement membrane region of intimal cells. In advanced lesions thick deposits of type IV collagen were found around the elongated smooth muscle cells. Using immunoelectron microscopy, type V collagen was found to be localized to cross-banded collagen fibers, and type VI collagen was found to be localized to beaded filaments present throughout the interstitium of the thickened intima. These findings suggest that collagens preserve the pathophysiological and functional integrity of the vascular wall by providing mechanical support as well as assuring the proper interaction of cells during the formation of atherosclerotic lesions. (Arteriosclerosis and Thrombosis 1992;12:494-502) KEYWORDS • atherosclerosis • collagen • immunohistochemistryA therosclerosis is a disease of large and medium-/ \ sized arteries and is characterized by focal X A . thickening of the inner portion of the artery wall in association with fatty deposits.1 -2 The common underlying events responsible for lesion formation are intimal smooth muscle cell proliferation, formation of new extracellular matrix by these cells, and the possible accumulation of lipid. Collagens are regarded as important in this disease not only because they represent the major extracellular component in the atherosclerotic plaque and thereby contribute to the occlusive nature of the disease but also because they may play an important role in hemostasis and thrombosis through stimulation of blood platelets. 3 In addition, recent studies have indicated that collagens can influence the proliferation and state of differentiation of smooth muscle cells in vitro.4 -6 Thus, it seems likely that collagens could play a vital role in the pathogenesis of this disease.Collagen is now recognized as a family of proteins of at least 13 genetically distinct subtypes, each of which has a unique tissue specificity. -9 Of these 13 collagen types, six (I, III, IV, V, VI, and VIII) are present in blood vessels. 10 All of these collagen types except type VIII collagen are known to be synthesized by smooth From the Department of Pathology, School of Medicine, Kanazawa University, K...

show abstract

“…Each MMP has a particular substrate specificity (Hulboy et al, 1997). Since the major extracellular matrix proteins in the human uterine cervix are collagen types I and III (Kleissl et al, 1978;Minamoto et al, 1987), it is important to study the expression and regulation of MMPs such as MMP-1, -8 and -13, which have substrate selectivity for collagen types I and III. Although the expression of MMP-1, -2, -3, -9 and -14 has been reported in cultured uterine cervical fibroblast (CxF) cells and/or smooth muscle cells by a number of investigators (Sato et al, 1996;Imada et al, 1997;Ito et al, 1998;Watari et al, 1999;Sugano et al, 2000), there have been few reports on the regulation of expression of MMPs in human uterine cervical tissue (Osmers et al, 1995a,b;Ledingham et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Prostaglandin F2alpha, cytokines and cyclic mechanical stretch augment matrix metalloproteinase-1 secretion from cultured human uterine cervical fibroblast cells

Yoshida

Sagawa²,

Itoh³

et al. 2002

Molecular Human Reproduction

View full text Add to dashboard Cite

Immunohistochemical studies on collagen types in the uterine cervix in pregnant and nonpregnant states

Cited by 75 publications

References 13 publications

Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

Mast cells degranulation affects angiogenesis in the rat uterine cervix during pregnancy

Collagens in human atherosclerosis. Immunohistochemical analysis using collagen type-specific antibodies.

Prostaglandin F2alpha, cytokines and cyclic mechanical stretch augment matrix metalloproteinase-1 secretion from cultured human uterine cervical fibroblast cells

Contact Info

Product

Resources

About