Immunohistochemical studies of the epididymal duct in Egyptian water buffalo (Bubalus bubalis)

Alkafafy, Mohamed; Elnasharty, Mohamed; Sayed-Ahmed, Ahmed; Abdrabou, Mohamed

doi:10.1016/j.acthis.2009.08.004

Cited by 18 publications

(34 citation statements)

References 62 publications

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“…The variable regional S100-IR displayed by PCs agrees with previous studies in the ox [2], donkey [7], and buffalo bull [9] epididymis. Additionally, ACs exhibited obvious S100-IR in the different epididymal regions.…”

Section: Discussionsupporting

confidence: 89%

“…Several studies have investigated the histology and histochemistry of the epididymal duct in different mammalian species [1-9]. However, data about the dromedary camel are still relatively limited [10-14].…”

Section: Introductionmentioning

confidence: 99%

“…In a continuing series of studies on the epididymal duct in different mammalian species, the current work used immunohistochemistry to underscore the spatial distribution of some biologically active proteins within the different regions of the epididymal duct and to highlight the potential structural-functional relationships. A comparative interpretation with other mammalian species (Table 1) [2, 7, 9] was also considered.…”

Section: Introductionmentioning

confidence: 99%

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Histological and immunohistochemical studies on the epididymal duct in the dromedary camel (Camelus dromedarius)

et al. 2011

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This study was conducted to underscore the spatial distribution of some biologically active proteins within the epididymal duct in the dromedary camel. Paraffin-embedded sections from different regions of epididymis were stained by conventional histological techniques and by immunohistochemistry. A battery of primary antibodies against six proteins (S100, alpha smooth muscle actin [α-SMA], connexin-43 [Cx43], galactosyltransferase [GalTase], angiotensin converting enzyme [ACE], and vascular endothelial growth factor [VEGF]) were used. The epididymal epithelium consisted of five cell populations: principal, basal, apical, dark, and halo cells. The histochemical findings indicated the absence of binding sites for VEGF and Cx43. The principal cells (PCs) showed variable immunoreactivity (IR) for ACE, S100, and GalTase throughout the whole length of the duct. The apical surfaces of most PCs (at the caput) and some PCs (at the corpus) exhibited intense ACE-IR, whereas those at the cauda displayed alternating negative and strong immunostaining. Similarly, moderate S100-IR was found in cytoplasm and nuclei of all PCs at the caput, few PCs at the corpus, and several PCs alternating with negative PCs at the cauda. In contrast, only some PCs showed weak to strong GalTase-IR in different regions. Apart from negative to weak positive S100-IR, basal cells failed to show IR for all other proteins. Apical cells displayed strong IR for ACE, S100, and GalTase with some regional differences. The peritubular and vascular smooth muscle cells revealed strong α-SMA-IR in all regions. In conclusion, the spatial distribution of different proteins in camel epididymis showed similarities and differences to other mammalian species. The region-specific topographic distribution of different proteins and cell types might indicate that the caput and cauda are metabolically more active than that of the corpus.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Histological and immunohistochemical studies on the epididymal duct in the dromedary camel (Camelus dromedarius)

et al. 2011

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“…These results are partially consistent with the previous reports of mammals (Table 3). S-100 protein is found in the BCs and PC of epididymis in human [30], bovine [23], donkey [34], buffalo [28], and camel [35]. Localization of S-100 is also seen in the ciliated cells of ED in bovine [23], and in the epididymal vascular endothelium of bison [33], bovine [23], and rat [25].…”

Section: Discussionmentioning

confidence: 99%

“…Despite all of these proposed functions, the exact biological role of this protein in the testis and epididymis is not yet known. The localization of S-100 was investigated in the testis and epididymis of several mammalian species including bovine [20, 21, 22, 23], sheep [21], rat [21, 24, 25], cat [21, 26], ram, boar, horse, dog [20, 21], buffalo [27, 28], monkey [29], and human [24, 30]. Conversely, one report concerning the S-100 localization in the testis and epididymis of adult White Peking ducks is, to our knowledge, available [31].…”

Section: Introductionmentioning

confidence: 99%

Localization of S-100 proteins in the testis and epididymis of poultry and rabbits

2014

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The present investigation was conducted to demonstrate S-100 protein in the testis and epididymis of adult chickens, Sudani ducks, pigeons, and rabbits. This study may represent the first indication for the presence of S-100 in the male reproductive organs of these species and might therefore serve as a milestone for further reports. In the testis of chickens, pigeons and rabbits, intense S-100 was seen in Sertoli cells. S-100 was also seen in the endothelial lining of blood vessels in rabbit testis. On the contrary, no S-100 reaction was detected in the Sertoli cells of Sudani ducks. In epididymis, the localization of S-100 had varied according to species studied; it was seen in the basal cells (BC) of epididymal duct in duck, non-ciliated cells of the distal efferent ductules in pigeons and ciliated cells of the efferent ductules and BC of rabbit epididymis. Conversely, S-100 specific staining was not detected in the epithelial lining of the rooster and pigeon epididymal duct as well as the principal cells of the rabbit epididymis. In conclusion, the distribution of the S-100 proteins in the testis and epididymis might point out to its roles in the male reproduction.

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Immunohistochemical studies on the poll gland of the dromedary camel (Camelus dromedarius) during the rutting season

2012

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Immunohistochemical studies of the epididymal duct in Egyptian water buffalo (Bubalus bubalis)

Cited by 18 publications

References 62 publications

Histological and immunohistochemical studies on the epididymal duct in the dromedary camel (Camelus dromedarius)

Histological and immunohistochemical studies on the epididymal duct in the dromedary camel (Camelus dromedarius)

Localization of S-100 proteins in the testis and epididymis of poultry and rabbits

Immunohistochemical studies on the poll gland of the dromedary camel (Camelus dromedarius) during the rutting season

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