Immunohistochemical studies of fetal trophoblast and maternal decidua in hydatidiform mole and choriocarcinoma

Bulmer, Judith N.; Johnson, Peter; Sasagawa, M; Takeuchi, Shoshichi

doi:10.1016/0143-4004(88)90016-1

Cited by 25 publications

(5 citation statements)

References 35 publications

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“…In the first trimester human decidua, 20–25% of the immune cells are Mϕs. Substantial numbers of these are closely associated with invasive EVTs throughout human pregnancy [27]. Mϕs are implicated in mediating both normal and abnormal placentation as well as in modulating the placental response to infection [28,29].…”

Section: Discussionmentioning

confidence: 99%

“…Mϕs are implicated in mediating both normal and abnormal placentation as well as in modulating the placental response to infection [28,29]. At the implantation site, recruited Mϕs accumulate adjacent to EVTs [27] where they phagocytose decidual apoptotic cells. The resulting remodeling of the decidua is proposed to promote EVT invasion [30].…”

Section: Discussionmentioning

confidence: 99%

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Pro-inflammatory cytokine-stimulated first trimester decidual cells enhance macrophage-induced apoptosis of extravillous trophoblasts

Yang

et al. 2012

Placenta

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Objective As human blastocyst-derived extravillous trophoblasts (EVTs) invade the early decidua, they are positioned to interact with immune cells and resident decidual cells, and remodel spiral arteries into high capacity vessels that increase blood flow to the developing fetal-placental unit. Shallow EVT invasion elicits incomplete vascular transformation and reduces uteroplacental blood flow that presages adverse pregnancy outcomes. Excess macrophages in the decidua induce EVT apoptosis via tumor necrosis factor-alpha (TNF-α) secretion. Our previous observation that pro-inflammatory cytokines enhance neutrophil and macrophage activator granulocyte-macrophage colony-stimulating factor (GM-CSF) expression in first trimester decidual cells is now extended to include: 1) the specific macrophage activator M-CSF; 2) macrophage activation and subsequent enhancement of EVT apoptosis by both GM-CSF and M-CSF. Study design Quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay assessed M-CSF expression in first trimester decidual cells incubated with interleukin-1 beta (IL-1β) or TNF-α. Peripheral monocyte-derived macrophages pre-incubated with conditioned media from decidual cell cultures were co-cultured with a first trimester EVT cell line, HTR-8/SVneo cells. Macrophage activation was examined and EVT apoptosis evaluated by DNA fragmentation, caspase activation and cell membrane asymmetry. Results IL-1β or TNF-α significantly enhanced M-CSF expression in first trimester decidual cells. The conditioned media from these cultures activates macrophages, which promote caspase 3/7-dependent EVT apoptosis with antibodies against GM-CSF or M-CSF blocking this effect. Conclusions Pro-inflammatory cytokines increases synthesis of M-CSF in first trimester decidual cells. Both GM-CSF and M-CSF activate macrophages, which initiate caspase-dependent EVT apoptosis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pro-inflammatory cytokine-stimulated first trimester decidual cells enhance macrophage-induced apoptosis of extravillous trophoblasts

Yang

et al. 2012

Placenta

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“…These two observations, namely of antigenic changes and an unusual mononuclear cell response, have suggested that the glands may be subject to immune destruction in early pregnancy. However, numerous glands have been demonstrated in third trimester placental bed tissues using antibodies reactive with epithelial cells, although the glands are often attenuated to form slit-like channels (Bulmer et al, 1986b (Bulmer et al, 1988d (Earl etal., 1987 ;Bulmer et al, 1987b (Golander et al, 1981 ;Nakayama et al, 1985) …”

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confidence: 99%

Immunoregulatory cells in human decidua : morphology, immunohistochemistry and function

Bulmer¹,

Pace²,

Ritson³

1988

Reprod. Nutr. Dévelop.

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134

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“…As was demonstrated by Sengupta et al [25], the M. Lambropoulou et al: Imbalance of mononuclear cell infiltrates in placental tissue granular infiltration of the decidua is not restricted to pregnancy; rather, it was part of the hormone-induced decidual response in ovariectomised rhesus monkeys. Moreover, Bulmer et al [26] concluded from their studies on decidua in molar pregnancy and on choriocarcinomas that these cells are associated with the hormonal conditions of decidualisation rather than with trophoblastic invasion. Correspondingly, they are also present in late proliferative and secretory endometrium, where they also may undergo mitosis [27].…”

Section: Discussionmentioning

confidence: 97%

Imbalance of mononuclear cell infiltrates in the placental tissue from foetuses after spontaneous abortion versus therapeutic termination from 8th to 12th weeks of gestational age

Lambropoulou

Tamiolakis²,

Venizelos

et al. 2006

Clin. Exper.Med.

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Placental macrophages (Hofbauer cells) are located close to trophoblastic cells and foetal capillaries, which make them perfect candidates for involvement in regulatory processes within the villous core. Their capacity of producing several cytokines and prostaglandin-synthesising enzymes, and expressing vascular endothelial growth factor, indicate a possible role in placental development and angiogenesis in order to support pregnancy. Common cells to Hofbauer macrophages sharing similar cell surface markers (HLA-A, -B, -C and leukocyte common antigen) have been reported in the stroma, decidua and amnion, indicating additional foetal protection. Yet this is not always the case. Most spontaneous abortions occur before 12 weeks' gestation, and most are due to chromosomal errors in the conceptus. Relatively few truly spontaneous abortions take place between 12 and 20 weeks' gestation. Thereafter, between 20 and 30 weeks, another type of premature spontaneous termination becomes prevalent, which is due to ascending infection. The numbers of cells expressing the various markers of the monocytemacrophage lineage change throughout pregnancy. In the present study, we investigated the immunohistochemical expression of mononuclear infiltrations in paraffin-embedded placentas, from foetuses after spontaneous abortion (8th, 10th and 12th weeks of gestational age), and those after therapeutic abortion at the same time, using a panel of monoclonal antibodies for the identification of leukocytes (CD45/LCA), B-lymphocytes (CD20/L-26), T lymphocytes (CD45RO/UCHL1), CD68 and CD14 cells. Immunologic factors in human reproductive failure are plausible mechanisms of infertility and spontaneous abortion. Approximately 25% of cases of premature ovarian failure appear to result from an autoimmune aetiology. Unfortunately, current therapeutic options for these women are limited to exogenous hormone or gamete substitution. Local inflammations at the sites of endometriosis implants are postulated to mediate the pain and reduced fecundability associated with this clinical syndrome. The recruitment of immune cells, particularly monocytes and T-cells, neovascularisation around foci of invading peritoneal lesions, and the possible development of antiendometrial autoantibodies support an immunologic basis of this disorder. To date, treatment of pain and infertility associated with endometriosis is primarily surgical, although immune-based adjuvants are theoretical possibilities for the future. Finally, although hypotheses supporting immunologic mechanisms of recurrent pregnancy loss have been popular over the past decade, most clinical investigations in this area do not provide compelling evidence for this position. Reputable specialists in reproductive medicine use experimental immunotherapies judiciously in selected cases of repetitive abortion. For example, the use of anticoagulation therapy can be beneficial in cases with documented antiphospholipid antibodies. At present, however, efficacious immunotherapy protocols for general application ...

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Immunohistochemical studies of fetal trophoblast and maternal decidua in hydatidiform mole and choriocarcinoma

Cited by 25 publications

References 35 publications

Pro-inflammatory cytokine-stimulated first trimester decidual cells enhance macrophage-induced apoptosis of extravillous trophoblasts

Pro-inflammatory cytokine-stimulated first trimester decidual cells enhance macrophage-induced apoptosis of extravillous trophoblasts

Immunoregulatory cells in human decidua : morphology, immunohistochemistry and function

Imbalance of mononuclear cell infiltrates in the placental tissue from foetuses after spontaneous abortion versus therapeutic termination from 8th to 12th weeks of gestational age

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