Immunohistochemical studies from vitiligo -comparison between active and inactive lesions-

Abstract

Vitiligo is an acquired, progressive depigmenting disorder of unknown etiology. In this study, to clarify pathogenesis of vitiligo, the marginal skin of actively spreading and stable vitiligo was examined using ICAM-1, HLA-DR, CD4 and CD8 monoclonal antibodies. In immunohistochemical study, ICAM-1 was expressed in four of five epidermis in active lesions, but not in stable lesion. Dermal ICAM-1 was also expressed in all active and stable lesions. HLA-DR was also expressed in all active epidermis in active lesions, but two of five epidermis in stable lesion. Dermal HLA-DR was also expressed in all active and stable lesion. CD4 lymphocytes were expressed more strongly in active lesion, but CD8 lymphocytes were not different in both lesions. There was no significant difference of degree of positivity with CD4 and CD8 in normal control specimens. In conclusion, we think that ICAM-1 and HLA-DR expression, cytokines released from keratinocytes, melanocytes or lymphocytes and infiltration of activated T-lymphocytes play an important role in disease activity.

The mRNA expression profile of cytokines connected to the regulation of melanocyte functioning in vitiligo skin biopsy samples and peripheral blood mononuclear cells

“…Furthermore, in patients with stable vitiligo, the ICAM1 expression has decreased in involved skin compared with uninvolved skin, which did not occur in case of patients with active vitiligo. These results are supported with previous findings [31,32,37]. Therefore, ICAM1 is probably an important link between cytokines and T cells involved in vitiligo pathogenesis.…”

“…Its mRNA expression has increased in vitiligo involved and uninvolved skin compared with control skin [15,29]. IFNG stimulates the expression of intercellular adhesion molecule 1 (ICAM1), which is important for activating T cells and recruiting leukocytes [30,31]. ICAM1 protein expression is upregulated in vitiligo skin and in melanocytes from perilesional vitiligo skin [32].…”

Imuno-histoquímica para o diagnóstico precoce de vitiligo

“…Outras alterações também podem ser observadas, como dermatite de interface com infiltrado linfocitário na derme superficial (13) . Estas alterações são mais proeminentes no vitiligo ativo do que no estável (1,6) . Nossos achados são semelhantes aos descritos na literatura, onde somente nos casos com evolução recente observou-se infiltrado inflamatório.…”

Systemic Corticosteroids in Vitiligo

“…Active lesions show increased numbers of CD4+ T cells and increased epidermal expression of ICAM-1. 13 Peripheral T-cell activation is also seen in nonsegmental vitiligo and T-cell dysregulation is not only limited to the lesions. 14 The peripheral blood of vitiligo patients express more of the cutaneous lymphocyte-associated antigen positive melanocyte-specific cytotoxic T lymphocytes indicating that there is recruitment of T cells in the circulation to the skin.…”