Immunohistochemical Staining Patterns of Canine Meningiomas and Correlation with Published Immunophenotypes

Abstract

Abstract. This study examined immunohistochemical staining patterns for several meningioma variants involving either the brain or spinal cord of dogs. Formalin-fixed, paraffin-embedded tissue from 15 tumors was obtained. The selected tumor group included seven meningothelial, three transitional, two malignant (anaplastic), one myxoid, one papillary, and one osteomatous meningiomas. Tumors were evaluated for reactivity to the following six immunohistochemical markers: vimentin, pancytokeratin, glial fibrillary acidic protein (GFAP), S100, neuron-specific enolase (NSE), and synaptophysin. Vimentin expression was detected in all meningiomas, and 14 of 15 tumors demonstrated intense vimentin staining in more than 50% of the neoplastic cells. Pancytokeratin expression was present in 11 of 15 neoplasms; however, positive staining frequently was focal and often involved a small percentage of the neoplastic cells. GFAP expression was detected in a single, anaplastic meningioma. Although expression of NSE and S100 was detected in 12 of 25 meningiomas, the intensity of the staining and the percentage of positive neoplastic cells was highly variable. Synaptophysin was uniformly negative. These results will help to establish immunohistochemical profiles for meningiomas that will improve our ability to correctly differentiate these neoplasms of meningeal origin from central nervous system tumors originating from other sites.

Intracranial Meningioma with Polygonal Granular Cell Appearance in a Chihuahua

“…This diagnosis is also supported by the histopathological and immunohistochemical findings previously reported in canine meningiomas [1,7]. The distribution of the lesions in the present case was different from that usually observed in canine meningiomas.…”

Expression of cytokeratin by malignant meningiomas: diagnostic pitfall of cytokeratin to separate malignant meningiomas from metastatic carcinoma

“…Meningioma has been reported to be immunoreactive for EMA, vimentin and S-100. 8,9,11,18,[20][21][22][23][24][25][26] Secretory meningiomas have been shown to be immunoreactive for cytokeratin and usually behave in a more aggressive clinical course than other benign meningiomas. 8,[11][12][13]21,27 There are 12 malignant meningiomas evaluated in this study.…”

Meningiomas em cães: aspectos clínicos, histopatológicos e imuno-histoquímicos

“…Há marcação forte e difusa para o filamento intermediário vimentina na maioria das células neoplásicas (Barnhart et al 2002). A marcação para citoqueratina é variável e frequentemente focal.…”