Immunohistochemical Stain-Phosphohistone H3: Most Specific Mitotic Marker

Sudarshini, N; Banavar, Spoorthi Ravi; Nambiar, Shwetha; Augustine, Dominic; Sowmya, S; Haragannavar, Vanishree C; Rao, Roopa S

doi:10.7860/jcdr/2018/24936.11208

Cited by 2 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is evident that certain architectural features may be consistently easier to detect (even at lower magnification) as compared to other features at cellular or nuclear level. The use of immunohistochemical markers, such as Phosphorylated Histone H3 (PHH3) and Ki67 can be considered as adjuncts for the assessment of mitosis and cell proliferation 21 , although more extensive evaluation of their usefulness as a prognostic indicator in OED is needed.…”

Section: Discussionmentioning

confidence: 99%

“…However, the department in question is a regional and national referral centre in the UK and therefore receives tissue samples from multiple hospitals covering a wide geographical area, thereby providing a sufficiently varied cohort for this pilot study. Furthermore, whilst the sample size may be considered small, it is larger than other studies which have explored OED analysis or proposed alternate OED grading classifications 12,16,21 . Nevertheless, application of these findings to substantially larger multicentre cohorts will allow more robust validation of the proposed potential prognostic models 31 .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Prediction of malignant transformation and recurrence of oral epithelial dysplasia using architectural and cytological feature specific prognostic models

et al. 2022

View full text Add to dashboard Cite

Oral epithelial dysplasia (OED) is a precursor state usually preceding oral squamous cell carcinoma (OSCC). Histological grading is the current gold standard for OED prognostication but is subjective and variable with unreliable outcome prediction. We explore if individual OED histological features can be used to develop and evaluate prognostic models for malignant transformation and recurrence prediction. Digitised tissue slides for a cohort of 109 OED cases were reviewed by three expert pathologists, where the prevalence and agreement of architectural and cytological histological features was assessed and association with clinical outcomes analysed using Cox proportional hazards regression and Kaplan–Meier curves. Within the cohort, the most prevalent features were basal cell hyperplasia (72%) and irregular surface keratin (60%), and least common were verrucous surface (26%), loss of epithelial cohesion (30%), lymphocytic band and dyskeratosis (34%). Several features were significant for transformation (p < 0.036) and recurrence (p < 0.015) including bulbous rete pegs, hyperchromatism, loss of epithelial cohesion, loss of stratification, suprabasal mitoses and nuclear pleomorphism. This led us to propose two prognostic scoring systems including a ‘6-point model’ using the six features showing a greater statistical association with transformation and recurrence (bulbous rete pegs, hyperchromatism, loss of epithelial cohesion, loss of stratification, suprabasal mitoses, nuclear pleomorphism) and a ‘two-point model’ using the two features with highest inter-pathologist agreement (loss of epithelial cohesion and bulbous rete pegs). Both the ‘six point’ and ‘two point’ models showed good predictive ability (AUROC ≥ 0.774 for transformation and 0.726 for recurrence) with further improvement when age, gender and histological grade were added. These results demonstrate a correlation between individual OED histological features and prognosis for the first time. The proposed models have the potential to simplify OED grading and aid patient management. Validation on larger multicentre cohorts with prospective analysis is needed to establish their usefulness in clinical practice.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Prediction of malignant transformation and recurrence of oral epithelial dysplasia using architectural and cytological feature specific prognostic models

et al. 2022

View full text Add to dashboard Cite

show abstract

“…The advent of novel technologies and translational research has also revolutionized the immunotherapeutic approach in oral cancer patients. However, it is observed that most of the immunotherapeutic studies published in the literature are on HNSCC, and there is a dearth of studies on OSCC [130][131][132][133][134][135][136]. Hence, the oral cancer field avidly awaits the clinicians and researchers provision of more insights to maximize the potential of immunotherapies in future endeavors.…”

Section: Discussionmentioning

confidence: 99%

Immunology of Oral Squamous Cell Carcinoma—A Comprehensive Insight with Recent Concepts

Venkatesiah

Augustine

Mishra

et al. 2022

Life

View full text Add to dashboard Cite

This review aims to understand the concept of oral cancer immunology through the notion of immune profiling, immunoediting and immunotherapy, and to gain knowledge regarding its application for the management of oral cancer patients. Oral cancer is an immunogenic tumor where the cells of the tumor microenvironment play an important role in tumorigenesis. Understanding the mechanism of these modulations can help design immunotherapeutic strategies in oral cancer patients. This article gives an overview of immunomodulation in the oral cancer tumor microenvironment, with concepts of immune profiling, immunoediting and immunotherapy. English literature searches via Google Scholar, Web of Science, EBSCO, Scopus, and PubMed database were performed with the key words immunology, tumor microenvironment, cells, cross talk, immune profiling, biomarkers, inflammation, gene expression, techniques, immunoediting, immunosurveillance, tumor escape, immunotherapy, immune checkpoint inhibitors, vaccines in cancer, oral cancer, and head and neck cancer. Original research articles, reviews, and case reports published from 2016–2021 (n = 81) were included to appraise different topics, and were discussed under the following subsections. Literature published on oral cancer immunology reveals that oral cancer immune profiling with appropriate markers and techniques and knowledge on immunoediting concepts can help design and play an effective role in immunotherapeutic management of oral cancer patients. An evaluation of oral cancer immunology helps to determine its role in tumorigenesis, and immunotherapy could be the emerging drift in the effective management of oral cancer.

show abstract

Immunohistochemical Stain-Phosphohistone H3: Most Specific Mitotic Marker

Cited by 2 publications

References 0 publications

Prediction of malignant transformation and recurrence of oral epithelial dysplasia using architectural and cytological feature specific prognostic models

Prediction of malignant transformation and recurrence of oral epithelial dysplasia using architectural and cytological feature specific prognostic models

Immunology of Oral Squamous Cell Carcinoma—A Comprehensive Insight with Recent Concepts

Contact Info

Product

Resources

About