Immunohistochemical profile of transforming growth factor-β1 and basic fibroblast growth factor in sciatic nerve anastomosis following pinealectomy and exogenous melatonin administration in rats

Turğut, Mehmet; Öktem, Gülperi; Uysal, Ayşegül; Yurtseven, Mine

doi:10.1016/j.jocn.2005.07.019

Cited by 13 publications

(10 citation statements)

References 43 publications

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“…The degree of positive staining for BMPs and BMPRs was evaluated by scoring intensity on a scale of 1-4 (I: none, mild, moderate, and strong), and distribution on a scale of 1-4 (D: none, focal patchy and diffuse). Tissue with I 9 D less than or equal to 4 were considered 'weakly positive', those with I 9 D greater than 4 were designated 'strongly positive' and those with no immunoreactivity were designated as 'absent' [27]. The intraobserver and interobserver variability studies were performed on 75 slides and by two observes.…”

Section: Methodsmentioning

confidence: 99%

Are BMPs Involved in Normal Nerve and Following Transection?: A Pilot Study

Tsujii

Akeda

Iino

et al. 2009

Clin Orthop Relat Res

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Bone morphogenic proteins (BMPs) may have neurotrophic functions but there is limited evidence of these functions in the peripheral nervous system. We therefore investigated the expression of BMPs and BMP receptors (BMPRs) in normal and injured peripheral nerves. In 10 of 15 Sprague-Dawley rats, a 3-mm segment of sciatic nerve was resected at the trifurcation in the thigh. One day (n = 5) and 7 days (n = 5) after transection, proximal and distal stumps were removed and immunohistochemically analyzed for BMP-2, -7, BMPR-1A, -1B, and -2. The other five animals served as normal controls. In normal nerves, BMP-2 expression was localized at Ranvier's node, and BMP-7 and BMPR-1B were expressed in several axon-Schwann cell units, whereas other receptors were not expressed. After nerve transection, BMP-7 expression was upregulated at both proximal and distal stumps along with Schwann cell columns during Wallerian degeneration. BMPRs were also upregulated compared with the normal nerve. The upregulation in BMP expression after nerve transection suggests that BMPs may play a role in the healing response of the peripheral nerve.

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Section: Methodsmentioning

confidence: 99%

Are BMPs Involved in Normal Nerve and Following Transection?: A Pilot Study

Tsujii

Akeda

Iino

et al. 2009

Clin Orthop Relat Res

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“…81 The role of TGF-β and bFGF collagen production and thus scar formation is known. In this regard, another study by Turgut et al 114 on pinealectomized rats revealed that expression of TGF-β1 and bFGF was suppressed by melatonin using immunohistochemical methods. In a study of pinealectomized chickens, Turgut et al 136 also examined the neuroprotective effects of melatonin.…”

Section: Comparison Of Beneficial Effects Of Melatonin and Some Agentmentioning

confidence: 98%

“…123 In addition, it was suggested that melatonin is neuroprotective at doses of 1-50 mg/kg. 85,114,122 Especially, Shokouhi et al 85 reported that melatonin at 50 mg/kg dose has a potentially positive effect on preserving the neural fibers. Similarly, Kaya et al 124,125 studied the effect of melatonin at 50 mg/kg dose on nerve injury.…”

Section: Controversies On Dose-dependent Effects Of Melatonin On Regementioning

confidence: 99%

Melatonin and sciatic nerve injury repair: a current perspective

Altunkaynak¹,

Delibaş²,

Altun³

et al. 2018

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Peripheral nerve injury is an important clinical problem that can exert hazardous effects on the health of patients. For this reason, there are more studies conducted on the regeneration of the peripheral nerves via the usage of the nerves belonging to various animals with different types of lesions, ages, and by using different methods of assessment with regular follow-up. Contrary to data obtained through experimentation and clinical observation, no ideal way of treatment was found to increase the regeneration of the peripheral nerves. Finally, the effects of melatonin in the protection of peripheral nerves against trauma, especially the protection of sciatic nerve from pathological conditions, have come into attention in a wide group of scientists as there are beneficial effects of melatonin after surgery. While numerous studies indicate the melatonin's protective effects on the pathologies of nerves, there are also studies reporting its toxic effects on peripheral nerves. Melatonin is a widespread and crucial signaling molecule due to its features of free radical scavenging and anti-oxidation at both pharmacological and physiological conditions in vivo. In this context, although there are numerous studies elaborating the effects of melatonin in various tissues, its effects on peripheral nerves was documented in only a limited number of studies. The aim of this article was to perform a review of the knowledge in the literature on the subject of mostly beneficial or hazardous effects of melatonin on the repair of the damaged peripheral nerves.

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“…A variety of growth factors may play role in collagen synthesis. The immunohistochemical profile of transforming growth factor (TGF-b1) and basic fibroblast growth factor (bFGF) in the lesion site of the sciatic nerve, after pinealectomy and exogenous melatonin administration, was investigated by Turgut et al [55]. They reported that TGF-b1 and bFGF immunoreactivity was most intense in the epineurium in animals that had undergone pinealectomy, while negative or weakly positive immunoreactivity was observed in animals in control and melatonin-treated groups [55].…”

Section: Effect Of Melatonin Treatment On Apoptosis and Neuronal Cellmentioning

confidence: 99%