Immunohistochemical profile of cytokeratins in pseudoepitheliomatous hyperplasia of cutaneous Leishmaniasis

Viana, Agostinho Gonçalves; Domingos, Patrícia Luciana Batista; Kaminagakura, Estela; Botelho, Ana Cristina Carvalho; Martelli, Hercílio; Bonan, Paulo Rogério Ferreti

doi:10.1111/j.1365-4632.2011.04888.x

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…An exuberant pseudoepitheliomatous hyperplasia may occur, which leads to a differential diagnosis with squamous cell carcinoma (Figure 7). 47 Particularly in mucosal leishmaniasis, the amastigote forms are very scarse. 42 …”

Section: Histopathological Exammentioning

confidence: 99%

Complementary exams in the diagnosis of american tegumentary leishmaniasis

Gomes

Paula

Morais

et al. 2014

An. Bras. Dermatol.

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The diagnosis of American Tegumentary Leishmaniasis is a difficult but essential task when considering the high toxicity profile of the drugs available. Since the discovery of its etiologic agent, numerous diagnostic tests have been developed. None of the tests available today can be considered as the gold standard, since they do not add enough accuracy for the disease detection. Good epidemiological and clinical knowledge of the disease are fundamental precepts of the dermatology practice and precede the rational use of existing diagnostic tests. In this article we aim, through extensive literature review, to recall fundamental concepts of any diagnostic test. Subsequently, based on this information, we will weave important comments about the characteristics of existing diagnostic tests, including immunological tests such as Montenegro's skin test, serology and detection of parasites by direct examination, culture or histopathology. Finally we will discuss the new technologies and options for the diagnosis of Cutaneous Leishmaniasis. The molecular biology technique is considered a promising tool, promoting the rapid identification of the species involved. We also aim to educate dermatologists about a disease with high morbidity and assist in its difficult recognition.

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Section: Histopathological Exammentioning

confidence: 99%

Complementary exams in the diagnosis of american tegumentary leishmaniasis

Gomes

Paula

Morais

et al. 2014

An. Bras. Dermatol.

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Immunohistochemical Expression of Keratins in Normal Ovine Skin and in Chronic Dermatitis due to Sarcoptes scabiei

Doukas

Liakou

Koukoulis

et al. 2021

Journal of Comparative Pathology

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Evaluation of myofibroblasts and its association with TGF-β and IFN-γ in lesions of patients with american tegumentary leishmaniasis

Viana

Bonan

2012

An. Bras. Dermatol.

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BACKGROUND: Leishmaniasis is caused by protozoa of Leishmania spp. genus. It is transmitted by the bite of the sand fly insect. It is believed that 12 million people are infected with this disease and that its annual incidence is 2 million; this number is increasing. OBJECTIVES: The present study aimed to evaluate the expression of myofibroblasts through alpha smooth muscle actin labeling, and to analyze their relationship with the expression of the cytokines Interferon gama (IFN-γ) and Transforming growth factor beta (TGF-β1) in lesions of American tegumentary leishmaniasis (ATL). METHODS: For this retrospective study, we gathered 28 patients diagnosed with ATL between 2002 and 2006. We verified α-SMA positivity and performed IFN-γ and TGF-β1 immunolabeling to identify the profile of these cytokines in both positive and negative cases for myofibroblasts, via immunohistochemistry, in order to assess the presence of myofibroblasts,. RESULTS: We observed that out of the 28 cases, 17 (60.71%) were positive for alpha smooth muscle actin, while 11 (39.29%) were negative, and IFN-γ was more expressed than TGF-β1 (p=0.007). The mean percentages of expression of IFN-γ and TGF-β1 in the group negative for alpha smooth muscle actin were different, with an increased expression of IFN-γ (p=0.047). However, in the group positive for alpha smooth muscle actin, there was no difference in cytokine labeling (p>0.05). CONCLUSION: We verified the presence of positive α-SMA stromal cells in the majority of the cases of ATL, indicating the presence of myofibroblasts. In cases negative for alpha smooth muscle actin, an increased expression of IFN-γ compared to TGF-β1 was observed, revealing an inflammatory phase progressing to a healing process.

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Immunohistochemical profile of cytokeratins in pseudoepitheliomatous hyperplasia of cutaneous Leishmaniasis

Abstract: In summary, we noticed that the pattern of some Ck staining in pseudoepitheliomatous hyperplasia in CL lesions revealed intense epithelial reinforcement, protection, and proliferation.

Cited by 3 publications

References 21 publications

Complementary exams in the diagnosis of american tegumentary leishmaniasis

Complementary exams in the diagnosis of american tegumentary leishmaniasis

Immunohistochemical Expression of Keratins in Normal Ovine Skin and in Chronic Dermatitis due to Sarcoptes scabiei

Evaluation of myofibroblasts and its association with TGF-β and IFN-γ in lesions of patients with american tegumentary leishmaniasis

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