Immunohistochemical observations on cellular response in unilocular hydatid lesions and lymph nodes of cattle

Sakamoto, Tsukasa; Cabrera, P.

doi:10.1016/s0001-706x(02)00226-7

Cited by 39 publications

(49 citation statements)

References 16 publications

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“…Also it has been shown that the smallest unilocular hydatid cysts were surrounded by an epithelioid cell layer composed of mononuclear cells bound to a laminated layer. These cells have usually been considered to be derived from macrophages (Sakamoto and Cabrera 2003). So variation in rate of IL4 expression in fibrous layer of hydatid cyst observed in our study may be related to the developmental stages of the cysts.…”

Section: Discussionmentioning

confidence: 52%

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IL-4 gene expression in adventitial layer (fibrous layer) of hepatic ovine and bovine hydatid cysts

et al. 2014

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Cystic Echinococcosis is a parasitic disease with cosmopolitan distribution caused by the tape worm Echinococcus granulosus. Fibrous layer is developed around the cyst as a host immune response reaction. The aim of this study was to evaluate the rate of IL-4 gene expression in fibrous layer of bovine and ovine hepatic hydatid cysts using quantitative technique of Real-Time PCR. In this descriptive study the samples of hydatid cyst fibrous layer were taken from 6 bovine and 6 ovine hepatic hydatid cysts. Samples of normal liver tissue close to the cyst were also taken as controls. Total RNA from each sample was extracted and then converted to cDNA. Afterward, the rate of IL-4 gene expression for each sample was evaluated using real-time PCR technique. Data were analyzed by REST software (version 2.0.13, 2009). In sheep the rate of IL-4 gene expression in the fibrous layer of hepatic hydatid cysts was 1.98 times more than the rate of IL4 gene expression in control samples, but the difference was not significant (P = 0.561). In cattle the rate of IL-4 gene expression in the fibrous layer of hepatic hydatid cysts was 9.84 times more than that of control samples which was statistically significant (P \ 0.001). With high rate of IL4 expression especially in fibrous layer of bovine hydatid cyst, it can be concluded that this interleukin may play an important role in host parasite relationship.

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Section: Discussionmentioning

confidence: 52%

“…So eosinophils infiltrating and their products such as IL4 in the fibrous layer may have an important role in formation of hydatid lesions in cattle (Sakamoto and Cabrera 2003).…”

Section: Discussionmentioning

confidence: 99%

IL-4 gene expression in adventitial layer (fibrous layer) of hepatic ovine and bovine hydatid cysts

et al. 2014

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“…A hiperplasia observada em áreas T-dependentes, assim como o maior número de folículos linfóides secundários observados após a segunda e terceira imunização, podem ser explicadas devido a que a resposta de células T após o segundo e terceiro desafio, geralmente é mais rápida e efetiva do que o desafio antigênico inicial, como consequência do aumento no número de células antígeno-específicas que respondem de forma mais eficiente que as células naive durante as respostas primárias (Resende 2003, Sakamoto & Cabrera 2003.…”

Section: Discussionunclassified

Resposta de linfonodos em bovinos inoculados a campo com a vacina recombinante rSBm7462 anti Rhipicephalus (Boophilus) microplus)

et al. 2014

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A dependência exclusiva de compostos químicos para o controle de Rhipicephalus (Boophilus) microplus tornou-se uma das maiores preocupações científicas e econômicas dos últimos anos, e como consequência, estão sendo realizadas pesquisas para o desenvolvimento de vacinas. O objetivo deste trabalho foi avaliar a resposta de linfonodos de bovinos imunizados a campo com o peptídeo rSBm7462 anti R. (B.) microplus. Foram utilizados 14 bovinos mestiços (Bos taurus x Bos indicus), com idades entre 4-10 meses, mantidos em duas propriedades rurais do norte do estado de Minas Gerais. Os animais receberam três imunizações do peptídeo rSBm7462, aplicados por via subcutânea, com intervalo de 30 dias. Após 15 dias de cada imunização, os linfonodos pré-escapulares foram coletados e fixados por 18 horas em formol. Posteriormente, foram incluídos em Paraplast e as amostras foram coradas pela técnica hematoxilina-eosina (HE) para a observação de eventos celulares. Para a identificação do antígeno nos linfonodos dos animais imunizados, foi realizada a técnica de imuno-histoquímica (IHQ) com o método peroxidase-anti-peroxidase (PAP). A resposta de linfonodos dos bovinos inoculados foi avaliada pelas análises de formação de centros germinais (CG), hiperplasia de cordões medulares (CM) e a presença do antígeno rSBm7462 em células PAP+, demonstrando que o peptídeo recombinante rSBm7462 induz uma resposta imune adaptativa T-dependente, caracterizada nos tecidos linfóides secundários pela formação de estruturas que conferem afinidade e memória imunológica.

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“…Each viable protoscolex (PSC) may develop into an adult worm if ingested by a definitive host or into a new cyst if disseminated in tissues of an intermediate host as the consequence of cyst rupture (secondary hydatidosis). Around intact cysts there is a remarkably scarce inflammatory reaction (Breijo et al 2008;Coltorti and Varela-Diaz 1974;Marco et al 2006;Sakamoto and Cabrera 2003).…”

Section: The Hydatid Cyst and Its Natural Historymentioning

confidence: 99%

“…Effector mechanisms include neutrophils and macrophage activation, nitric oxide (NO), eosinophil cationic protein, complement, and antibodies (Virginio et al 2007;Amri et al 2007;Ferreira et al 2000a;Heath et al 1994;Jenkins et al 1990;Ramos et al 2006;Ferragut and Nieto 1996;Severi et al 1997;Zhang et al 2003b;Riley et al 1986;Dempster et al 1992). Around live intact cysts there is a remarkably scarce inflammatory reaction, as opposed to regressive cysts (Breijo et al 2008;Coltorti and Varela-Diaz 1974;Marco et al 2006;Sakamoto and Cabrera 2003). Although it is not clear whether the inflammatory reaction is the cause of cyst inactivation or the consequence of hydatid cyst fluid (HCF) spillage from spontaneously damaged cysts, some evidence exists that cysts can be damaged by an early pericystic inflammatory reaction (Fotiadis et al 1999).…”

Section: Immune Response To E Granulosusmentioning

confidence: 99%

Echinococcosis

Tamarozzi

Brunetti

Vuitton

2014

Helminth Infections and Their Impact on Global Public Health

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Cystic echinococcosis (CE) and alveolar echinococcosis (AE) are zoonoses of great medical and veterinary importance, caused by Echinococcus granulosus and E. multilocularis, respectively. The life cycle of these parasites develops between the dog and other canids, which harbor the adult tapeworm in the intestine, and mammal intermediate hosts (including humans as dead-end occasional hosts) where the larval form, or metacestode, develops in different organs.The impact of CE and AE on human health is important, with an estimated 1.2 million people affected and 3.6 million DALYs lost globally for CE and 666,434 DALYs for AE.We describe epidemiology, host's immune response to parasite, clinical manifestations, diagnosis, and treatment of both CE and AE.

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Immunohistochemical observations on cellular response in unilocular hydatid lesions and lymph nodes of cattle

Cited by 39 publications

References 16 publications

IL-4 gene expression in adventitial layer (fibrous layer) of hepatic ovine and bovine hydatid cysts

IL-4 gene expression in adventitial layer (fibrous layer) of hepatic ovine and bovine hydatid cysts

Resposta de linfonodos em bovinos inoculados a campo com a vacina recombinante rSBm7462 anti Rhipicephalus (Boophilus) microplus)

Echinococcosis

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