Immunohistochemical Localization of Type I, III, IV, V, and VI Collagens and Laminin in Neurofibroma and Neurofibrosarcoma

Chanoki, Miyako; Ishii, Masamitsu; Fukai, Kazuyoshi; Kobayashi, Hiromi; Hamada, Toshio; Muragaki, Yasuteru; Ooshima, Akira

doi:10.1097/00000372-199108000-00007

Cited by 23 publications

(13 citation statements)

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“…On the contrary, HSNF was able to compensate this deformation. This suggests that the collagen network is irregularly arranged in NF1, as reported in other studies [13,14] , and that the alterations of the collagen network seem to be more important for NF1 than HSNF.…”

Section: Discussionsupporting

confidence: 80%

“…Also, an important production of types III and VI collagen was observed; this production was noticed in keloid hypertrophic scars as well [26] . Normally, type IV collagen is produced in large amounts by Schwann cells, which are present indeed in NF1 [13] . Moreover, it was shown that the proteoglycan/collagen ratio was 4-10 times greater in NF1 than in the surrounding dermal tissue, which explains the typical soft consistency of the tumor and the reason why it could contribute to create a favorable tumor growth milieu [27] .…”

Section: Discussionmentioning

confidence: 99%

“…Fibroblasts are not an exception from this excessive proliferation rate, as reported in several publications demonstrating an extended proliferation of fi broblasts in NF1, which led to an increased production and deposition of collagen, the essential component of skin elasticity [29][30][31] . Furthermore, Schwann cell-like cells, in excessive proliferation, produce in particular type IV collagen and the laminin present around the tumor [13] . Our results suggest that this phenomenon could exist not only in NF1 but also in HSNF; both of them presented a hyperextensibility behavior, but contrary to HSNF, NF1 was unable to return to its initial position as a result of its abnormal deformation, thus suggesting that the tumor collagen network was more disorganized than the adjacent skin.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Skin Viscoelasticity in Type 1 Neurofibromatosis Patients

Mimoun

Razzouq²,

Wolkenstein³

et al. 2005

Skin Pharmacol Physiol

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Neurofibromatosis type 1 (NF1) is a frequent autosomal dominant disease characterized by cutaneous benign tumors called neurofibromas. Surgery takes an important place in managing these skin disorders. However, skin distensibility and softness of NF1 patients quickly offset the surgical benefit. The aim of this study was to determine the rheological behavior of neurofibromas and compare it with healthy skin in an attempt to comprehend what leads to this phenomenon. Thirty patients were admitted to this study. A group of 24 healthy control subjects was also included. The skin elasticity was assessed by a noninvasive in vivo suction device (Cutometer) including 5 consecutive suctions. The assessments were performed on neurofibroma skin, the supposedly healthy skin around neurofibromas and the healthy skin of control subjects. The extensibility at the first and the fifth traction in NF1 patients (neurofibromas and the supposedly healthy skin around it) was significantly different compared to the healthy skin of control subjects. The viscoelastic parameters obtained from the neurofibromas were significantly different in comparison to those obtained from the supposedly healthy skin of NF1 patients and the healthy skin of control subjects. The rheological profiles of the neurofibromas and the apparent healthy skin of NF1 patients demonstrated a hyperextensibility behavior, but in neurofibromas, the skin was unable to return to its initial position at the end of the stretch.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Skin Viscoelasticity in Type 1 Neurofibromatosis Patients

Mimoun

Razzouq²,

Wolkenstein³

et al. 2005

Skin Pharmacol Physiol

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“…Like human NF1 plexiform neurofibroma, intraneural sNF94.3 xenografts displayed hypocellularity with widely-spaced spin-dle-shaped cells, a low proliferative index, an extracellular matrix-rich stroma and basal laminae. Neurofibromas and MPNST have been shown previously to produce laminin (Chanoki et al, 1991). Schwann cells require the presence of other cell types, such as axons and fibroblasts, to produce basal laminae (Obremski et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Plexiform‐like neurofibromas develop in the mouse by intraneural xenograft of an NF1 tumor‐derived Schwann cell line

Perrin

Fishbein

Thomson

et al. 2007

J of Neuroscience Research

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Plexiform neurofibromas are peripheral nerve sheath tumors that arise frequently in neurofibromatosis type 1 (NF1) and have a risk of malignant progression. Past efforts to establish xenograft models for neurofibroma involved the implantation of tumor fragments or heterogeneous primary cultures, which rarely achieved significant tumor growth. We report a practical and reproducible animal model of plexiform-like neurofibroma by xenograft of an immortal human NF1 tumor-derived Schwann cell line into the peripheral nerve of scid mice. The S100 and p75 positive sNF94.3 cell line was shown to possess a normal karyotype and have apparent full-length neurofibromin by Western blot. These cells were shown to have a constitutional NF1 microdeletion and elevated Ras-GTP activity, however, suggesting loss of normal neurofibromin function. Localized intraneural injection of the cell line sNF94.3 produced consistent and slow growing tumors that infiltrated and disrupted the host nerve. The xenograft tumors resembled plexiform neurofibromas with a low rate of proliferation, abundant extracellular matrix (hypocellularity), basal laminae, high vascularity, and mast cell infiltration. The histologic features of the developed tumors were particularly consistent with those of human plexiform neurofibroma as well. Intraneural xenograft of sNF94.3 cells enables the precise initiation of intraneural, plexiform-like tumors and provides a highly reproducible model for the study of plexiform neurofibroma tumorigenesis. This model facilitates testing of potential therapeutic interventions, including angiogenesis inhibitors, in a relevant cellular environment.

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“…Silver and gold stains developed to mark normal cells of the neuropil have a reputation of unpredictability. 41 More frequently used in human neuropathology is the reticulin stain to identify basal lamina in meningiomas and nerve sheath tumors 14,58 and stains that detect argyrophilic nucleolar organizing regions, which, though cumbersome to quantitate, can be used to separate more from less aggressive parenchymal and meningeal tumors. 102 PTAH (phosphotungstic acid-hematoxlyn) has been used to stain the basal bodies of cilia in ependymomas.…”

mentioning

confidence: 99%

Diagnostic Immunohistochemistry of Canine and Feline Intracalvarial Tumors in the Age of Brain Biopsies

2013

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The focus of immunohistochemistry as applied to nervous system tumors is in identifying the neoplasm present and evaluating margins between normal and neoplastic tissue. Although not always utilized by specialists in neuropathology, immunohistochemistry remains useful to resolve concerns about the differentiation and rate of tumor growth. The aims of this review are to discuss the utility of immunohistochemical reagents currently used in diagnosis of canine and feline intracalvarial tumors, to indicate the applicability of some tests currently used in human nervous system tumors for domestic species, and to evaluate a few less commonly used reagents. A panel of biomarkers is usually needed to confirm a diagnosis, with groups of reagents for leptomeningeal, intraparenchymal, and ventricular neoplasms. In the future, signature genetic alterations found among feline and canine brain tumors-as correlated prospectively with diagnosis, rate of enlargement, or response to treatment-may result in new immunohistochemical reagents to simplify the task of diagnosis. Prospective studies determining the type and proportion of stem cell marker expression on patient longevity are likely to be fruitful and suggest new therapies. Due to increased frequency of biopsy or partial resection of tumors from the living patient, biomarkers are needed to serve as accurate prognostic indicators and assist in determining the efficacy of developing therapeutic options in nervous system tumors of dogs and cats.

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Immunohistochemical Localization of Type I, III, IV, V, and VI Collagens and Laminin in Neurofibroma and Neurofibrosarcoma

Cited by 23 publications

References 0 publications

Evaluation of Skin Viscoelasticity in Type 1 Neurofibromatosis Patients

Evaluation of Skin Viscoelasticity in Type 1 Neurofibromatosis Patients

Plexiform‐like neurofibromas develop in the mouse by intraneural xenograft of an NF1 tumor‐derived Schwann cell line

Diagnostic Immunohistochemistry of Canine and Feline Intracalvarial Tumors in the Age of Brain Biopsies

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