Immunohistochemical localization of thyroid hormone in rat thyroid gland.

Wilson, Marjorie; Hitchcock, Karen R.; DeLellis, Ronald A.

doi:10.1177/26.12.366016

J Histochem Cytochem.

1978

DOI: 10.1177/26.12.366016

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Immunohistochemical localization of thyroid hormone in rat thyroid gland.

Marjorie Wilson¹,

Karen R. Hitchcock²,

Ronald A. DeLellis³

Abstract: Direct and indirect immunofluorescence techniques were used to localize the thyroid hormones triidothyronine (T3) and thyroxine (T4) in adult rat thyroid gland. Optimum dilutions of the antisera were established and four tissue fixatives were investigated for usefulness in this technique. Use of antibodies specific for either T3 or T4 resulted in brilliant fluorescence in the colloid pools and apical cytoplasm of follicular cells. In all cases, the adjacent parathyroid gland was devoid of fluorescence. This re… Show more

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1986

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Cited by 9 publications

(3 citation statements)

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“…However, there are only a few studies regarding the localization of T3 and T4 and these have contradictory results. Wilson et al (1978) reported that after fixation in absolute methanol, immunoreactive T3 is observed within the colloid and apical cytoplasm of the follicular cells, whereas Kawaoi et al (1981) have written that after fixation in 10% formalin solution, T3 localization is observed in the cytoplasm of follicular cells rather than in the colloid. In addition, there is no information on the development of thyroid hormones by immunocytochemical means.…”

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confidence: 99%

Localization and development of immunoreactive triiodothyronine in thyroid glands of dogs and chickens

et al. 1986

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The localization of immunoreactive T3 was investigated in dog and chick thyroid glands either fixed in Bouin's solution or freeze-dried and fixed with paraformaldehyde vapor, and compared with localization of 19S-thyroglobulin. Freeze-drying followed by paraformaldehyde vapor was better for the demonstration of T3 than Bouin's solution; it gave a much stronger immunoreactivity for T3. This fixative was also excellent for the demonstration of thyroglobulin. The immunoreactive T3 was detected only in the colloid and was never observed in the follicular cells, although immunoreactive thyroglobulin was present not only in the colloid but also in the follicular cells. Subsequently, in dog fetuses and chick embryos the appearance and development of immunoreactive T3 were studied. At 40 days of gestation in dog fetuses and at 9 days of egg incubation in chick embryos, immunoreactive T3 was found in the colloid of primordial follicles coinciding with the formation of the colloid. The ability of embryonic thyroid glands to synthesize T3 seems to be linked to the organization of follicles. With progressing development, the follicles stored more colloid and immunoreactive T3 within the follicular lumina. Concentrations of immunoreactive T3 in thyroids from chickens at various stages of development were also studied by radioimmunoassay. The T3 concentration was first detected at 9 days of incubation and gradually increased with embryo age; it was related to the amounts of colloid stored in the follicles.

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Localization and development of immunoreactive triiodothyronine in thyroid glands of dogs and chickens

et al. 1986

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“…Probably the first immunohistochemical localization of thyroid hormones T4 and T3 was the one reported by Wilson et al in 1978 employing the fluorescent antibody method on frozen sections of the rat thyroid gland (25). We clarified that it is possible to detect T4 and T3 by using thyroid tissue fixed with formalin and embedded in paraffin (7, 8).…”

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confidence: 90%

“…Regarding hormones other than protein hormones, such as thyroid hormones T4 and T3, steroid hormones, bioactive amines and prostaglandins, on the other hand, some attempts to clarify their localizations have been made, but research into their localization or distribution is still in the developmental stage (1,6,25).…”

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confidence: 99%

Recent progress and pathological application of hormone immunohistochemistry.

Kawaoi

1981

Acta Histochem. Cytochem

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Hormones and the lung II. Immunohistochemical localization of thyroid hormone binding in type II pulmonary epithelial cells clonally‐derived from adult rat lung

et al. 1979

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The type II pulmonary epithelial cell is the recognized state of surfactant synthesis and storage. Results of recent studies indicate that the thyroid hormones, triiodothyronine (T3) and thyroxine (T4), may be important regulators of surfactant production and/or release. Direct and indirect immunofluorescence techniques were used in an attempt to demonstrate binding of T3 and T4 in monolayer cultures of isolated type II cells. These cultured epithelial cells are clonally-derived from adult rat lung, retain a diploid karyotype through 35 population doublings in vitro, contain granular inclusions (lamellar bodies) in the perinuclear cytoplasm, and synthesize phosphatidylcholine via the CDP-choline pathway. In isolated type II cells, either of two fluorescent patterns was observed: (a) nuclear fluorescence accompanied by a reticular perinuclear network; or (b) diffuse cytoplasmic accumulations with concentrations around perinculear cytoplasmic inclusions. Ultrastructurally these inclusions had the typical appearance of lamellar bodies. Histochemical studies demonstrated that these inclusions contained surfactant-associated nonspecific esterases and stained with Nile blized markers for pulmonary surfactant indicate that these inclusions are indeed lamellar bodies, the putative sites of surfactant synthesis and/or storage. These findings suggest that the type II pulmonary epithelial cell contains specific binding sites for thyroid hormones, and support the hypothesis that thyroid hormones are regulators of surfactant metabolism.

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Immunohistochemical localization of thyroid hormone in rat thyroid gland.

Cited by 9 publications

References 5 publications

Localization and development of immunoreactive triiodothyronine in thyroid glands of dogs and chickens

Localization and development of immunoreactive triiodothyronine in thyroid glands of dogs and chickens

Recent progress and pathological application of hormone immunohistochemistry.

Hormones and the lung II. Immunohistochemical localization of thyroid hormone binding in type II pulmonary epithelial cells clonally‐derived from adult rat lung

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