Immunohistochemical localization of the rabbit haemorrhagic disease viral antigen

Alexandrov, M.; Peshev, R.; Yanchev, I.; Bozhkov, S; Doumanova, L.; Dimitrov, Todor; Zacharieva, S.

doi:10.1007/bf01309598

Cited by 15 publications

(8 citation statements)

References 21 publications

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“…CARRASCO et al (1991b) identified, by immunohistology, the presence of RHDV in the pulmonary intravascular macrophages (PIMs) of rabbits that had died from experimental RHDV transmission. Other reports have confirmed that, following fatal rabbit haemorrhagic disease, in the lung found to be positive for RHDV the virus was exclusively localized in the cytoplasm of PIMs (ALEXANDROV et al, 1992;CARRASCO et al, 1991b;STOERCKLE-BERGER et al, 1992). Therefore, the RHDV R N A found in the lung by RT-PCR in this study may also be related to the presence of virus in the PIMs.…”

Section: Discussionsupporting

confidence: 84%

“…In previous studies in rabbits mortally diseased with RHD, the liver was always found to be infected with RHDV, but the virus was only occasionally found in other organs (spleen, lung and kidney;ALEXANDROV et al, 1992;CARRASCO et al, 1991a;SMID et al, 1989;STOERCKLE-BERGER et al, 1992). This non-detection of the virus in the organs of rabbits that died from R H D may be related to the use of techniques such as electron microscopy and immunohistochemistry, which are less sensitive than the PCR method.…”

Section: Discussionmentioning

confidence: 89%

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Early Stages of Rabbit Haemorrhagic Disease Virus Infection Monitored by Polymerase Chain Reaction

Guittré¹,

Ruvoën-Clouet²,

Barraud³

et al. 1996

Journal of Veterinary Medicine, Series B

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Summary In order to define more accurately the initial events that take place during rabbit haemorrhagic disease virus (RHDV) infection, different organs of experimentally infected rabbits were analysed for the presence of the virus and correlated with histopathological observations. A total of 24 rabbits were intranasally inoculated with a viral suspension, and tissue samples were taken from the liver, spleen, kidney, lung, thymus, lymph node and tonsil at different intervals post‐inoculation (2, 4, 6, 12, 18, 24, 30, 36, 48, 50, 51, 70 and 72 h). Histopathological observations revealed the presence of the first significant lesions at 30 h post‐inoculation (p.i.) in the liver. Using an ELISA and a haemagglutination test (HAT), the virus was detected in the liver at 36 h p.i. The reverse transcriptase‐polymerase chain reaction (RT‐PCR) showed that the RHDV RNA was present as early as 18 h p.i. in the liver and spleen, whereas thymus, kidney, tonsil and lymph node were found to be positive after more than 36 h p.i. The lungs presented a variable positivity between 0 and 36 h p.i., but remained positive after this time.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 89%

Early Stages of Rabbit Haemorrhagic Disease Virus Infection Monitored by Polymerase Chain Reaction

Guittré¹,

Ruvoën-Clouet²,

Barraud³

et al. 1996

Journal of Veterinary Medicine, Series B

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“…All these results suggested that the renal impairment in RHD might be secondary to hepatic injury. The replication of RHDV occurred only in hepatocytes, Kupffer cells and splenic and alveolar macrophages [1,10], suggesting that macrophage migration and efflux of hepatic cell debris might contribute to the dissemination of RHDV from liver to kidney. Serum BUN/CREA ratio increased, because of urea reabsorption is increasing relative to an overall reduction in blood flow through the kidney.…”

Section: Discussionmentioning

confidence: 99%

Impairment of Renal Function and Electrolyte Balance in Rabbit Hemoorhagic Disease

Chen

Chou

Liu

et al. 2008

The Journal of Veterinary Medical Science

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ABSTRACT. Rabbit hemorrhagic disease virus (RHDV) induced viral fulminant hepatitis in adult rabbits. We investigated the damage of renal function and electrolyte balance in experimentally infected rabbit by measuring the related serum parameters to elucidate the pathogenesis of RHDV as an index for medical treatment. Nineteen New Zealand White rabbits, ten females and nine males, were each intramuscularly inoculated with 0.5 ml 50% rabbit lethal dose (RLD 50 ) rabbit hemorrhagic disease virus. Blood samples were collected at 0 hr post inoculation (HPI) and every 6 hr from 18 HPI repeatedly through 66 HPI. After virus inoculation, serum blood urea nitrogen (BUN), creatinine (CREA) and sodium (Na + ) were elevated to a highly significant level (p<0.0001), whereas serum potassium (K + ) was moderately elevated to a significant level (p<0.05). Hypoglycemia developed highly significantly (p<0.0001). Serum chloride ion (Cl -) was the only parameter which did not change significantly (p=0.077). No significant sexual difference was observed among these parameters. Renal insufficiency progressed from 36 hr, as indicated by the increases in BUN and CREA; significant changes in electrolytes resulting in the increased osmolality of extracellular fluid that induced flow disturbance which consequently destroy the homeostasis in cells. Therefore, the later impairments in renal function and electrolyte balance might be an important threat for rabbits which might have survived from acute fulminant hepatitis in RHD. KEY WORDS: electrolyte balance, rabbit hemorrhagic disease virus, renal insufficiency, serum.J. Vet. Med. Sci. 70(9): 951-958, 2008 Rabbit hemorrhagic disease (RHD) was first reported in 1984 in the People's Republic of China [12] and widespread worldwide to Europe, Australia, Mexico, North Africa and New Zealand in the following years. In 1994, RHD was first recognized in Taiwan by Dr. Y. S. Lu through serological tests and was characterized by hemagglutination, electron microscopic examination, viral protein analysis and RT-PCR in 1998 [20]. The characteristic pathological signs for RHD were hemorrhages in the respiratory system, liver, spleen, cardiac muscle, and occasionally in the kidneys. Incubation period in rabbits is 2 to 3 days, morbidity approaches 100%, and mortality is sometimes over 90% in adults. The high morbidity and mortality rates have made RHD a big threat for rabbit industry and also a biological control tool for wild rabbit population. The causative virus, rabbit hemorrhagic disease virus (RHDV), was classified into the family Caliciviridae. This single-stranded nonenvelope RNA virus has a 7.5-kb genome and a 2.2 kb subgenome well packaged in a 27 to 35 nm (in diameter) icosahedral capsid with a major structural protein of about 60 kDa (VP60), and 10 peripheral cup-shaped depressions [12,20]. The ability to agglutinate human erythrocytes of group O, A, B and AB were reported [12,15,20].Detection by reverse transcriptase-polymerase chain reaction (RT-PCR) in a rabbit inoculation model showed t...

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“…The electron microscopic investigation of two peaks showed full and empty viral particles with a diameter of 27 to 40nm (1). Analysis of structural proteins of RHDV in SDS-PAGE ~amples containing 10 to 15 J.lg protein purified by sucrose density gradient demonstrated four visible bands, corresponding to the proteins with molecular weights approximately 60K, 52K, 38K and 15K (data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…The intact virion is composed of 32 capsomers including 12 pentamers, 20 hexamers and 180 subunits (26). Different authors have established that it has a diameter of 32-40 nm (1,4,9,13,17,19,27). The sedimentation coefficient according to the methods used is: 153 S (4)or 200 S (9).…”

Section: Introductionmentioning

confidence: 99%

Purification and Biochemical Characteristics of the Rabbit Haemorrhagic Disease Virus

Veleva¹,

Peshev²,

Bostandjieva³

1995

Biotechnology & Biotechnological Equipment

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Immunohistochemical localization of the rabbit haemorrhagic disease viral antigen

Cited by 15 publications

References 21 publications

Early Stages of Rabbit Haemorrhagic Disease Virus Infection Monitored by Polymerase Chain Reaction

Early Stages of Rabbit Haemorrhagic Disease Virus Infection Monitored by Polymerase Chain Reaction

Impairment of Renal Function and Electrolyte Balance in Rabbit Hemoorhagic Disease

Purification and Biochemical Characteristics of the Rabbit Haemorrhagic Disease Virus

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