Immunohistochemical Localization of Prolactin-Binding Sites in R3327 Rat Prostatic Cancer Cells

Witorsch, Raphael J.

doi:10.1159/000179008

Cited by 8 publications

(2 citation statements)

References 19 publications

(36 reference statements)

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“…Among these, prolactin (PRL) has been demonstrated to exert significant stimulatory effects on the growth of various tumours such as breast (Manni et al 1985), liver (Buckley et al 1985) and prostate cancer (Johnson et al 1985), where specific PRL receptors have been identified (Witorsch 1979, Turcot-Lemay & Kelly 1982, Di Carlo et al 1984, Boutin et al 1989. Recently, it has been reported that PRL stimulates the proliferation of human meningioma cells in vitro (Jimenez-Hakim et al 1993, Boyle-Walsh et al 1995) and a possible involvement of the hormone has been suggested in the growth of this tumour.…”

Section: Introductionmentioning

confidence: 99%

Prolactin receptors in human meningiomas: characterization and biological role

Muccioli¹,

Ghè²,

Faccani³

et al. 1997

Journal of Endocrinology

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Sixty cerebral meningioma specimens obtained at surgery from 34 female and 26 male patients were examined for the presence of prolactin (PRL) receptors. These were compared with normal arachnoid tissue from which these tumours arise. PRL receptors were detected in 61.7% of meningiomas whereas no PRL binding was found in samples of normal arachnoid tissue. No relationship was found when sex or histological findings were compared with the presence of PRL receptors. Receptor-positive tumours had saturable and high-affinity (Kd, 4.8 +/- 0.5 ng/ml) receptors with hormonal specificity for human PRL (hPRL) resembling that of other target tissues of PRL in man. The biological role of these receptors was investigated in primary cell cultures derived from meningioma tissue characterized for PRL receptor. When human PRL was added to the culture medium, in doses ranging from 1 to 200 ng/ml, a dose-dependent stimulation of 3H-thymidine incorporation was observed only in PRL-receptor positive tumours. The PRL concentrations required to produce a half-maximal effect ranged from 11 to 20 ng/ml and were quite close to the dissociation constant (Kd) of binding of PRL to its receptors. PRL also caused an increase of cell number compared with control with a significant effect after 3 and 4 days of culture. In conclusion, these findings indicate that a large number of human meningiomas express specific and functional receptors for PRL which are involved in mediating its proliferative effects.

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Section: Introductionmentioning

confidence: 99%

Prolactin receptors in human meningiomas: characterization and biological role

Muccioli¹,

Ghè²,

Faccani³

et al. 1997

Journal of Endocrinology

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“…In addition to autoradiographic localization [14], PRL receptors have been identified by immunocytochemistry [15] in testes [16], mammary gland [17], ovary [18], and prostate [19][20][21].…”

Section: Introductionmentioning

confidence: 99%

Visualization of Gene Expression of Short and Long Forms of Prolactin Receptor in Rat Reproductive Tissues

Ouhtit

Morel

Kelly

1993

Biology of Reproduction

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Prolactin receptor gene expression was visualized in various tissues by in situ hybridization. Probes specific to the intracellular domains of the short and long form of receptor were prepared. The specificity of these signals was controlled by competition with excess unlabeled homologous probes or heterologous probes; moreover, some tissues, such as penis and vagina, show no expression of either form of receptor mRNA. Macroautoradiogram signals (optical density) were quantified and expressed in arbitrary units. The long form of receptor mRNA was preferentially expressed in testis, epididymis, prostate, seminal vesicle, and mammary gland from lactating animals, whereas the expression of the two forms of mRNA was equivalent in ovary, uterus, and pregnant mammary gland. Signals were also localized at the light microscopic level to individual cells. This approach has permitted the precise localization of prolactin receptor mRNAs in reproductive tissues. Actions of prolactin have not been demonstrated in all tissues expressing receptor transcripts; thus it will be interesting to correlate the expression of long and short forms of receptor with specific functions.

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Effect of perphenazine on growth and zinc‐65 uptake of the rat prostatic adenocarcinoma, R 3327

Rosoff

Diamond

1982

The Prostate

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Prolactin affects the growth and function of both normal and carcinatomous prostate tissue. Therefore, the effect of modifying prolactin secretion on the growth of the adenocarcinoma of the rat prostate (R3327) was studied, utilizing chronic administration of the dopamine antagonist perphenazine. At 4 and 8 weeks after tumor inoculation, perphenazine (0.5 and 1.0 mg per dose) was injected for 12-16 weeks. Tumors weighed at autopsy showed that the 0.5 mg dose resulted in significantly smaller and the 1.0 mg dose, in significantly larger tumors than the controls. Perphenazine at both doses increased serum prolactin, but the increase was greater when 0.5 mg was administered. There was a higher uptake of 65Zn when the tumor growth was inhibited as in the animal receiving 0.5 mg of perphenazine. Several possible explanations are offered for the results, including the fact that prolactin might follow a bell-shaped curve in its effect on the tumor and that perphenazine might have other mechanisms of action besides dopamine antagonism.

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Immunohistochemical Localization of Prolactin-Binding Sites in R3327 Rat Prostatic Cancer Cells

Cited by 8 publications

References 19 publications

Prolactin receptors in human meningiomas: characterization and biological role

Prolactin receptors in human meningiomas: characterization and biological role

Visualization of Gene Expression of Short and Long Forms of Prolactin Receptor in Rat Reproductive Tissues

Effect of perphenazine on growth and zinc‐65 uptake of the rat prostatic adenocarcinoma, R 3327

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