Immunohistochemical localization of neurocan and L1 in the formation of thalamocortical pathway of developing rats

Fukuda, Tetsuya; Kawano, Hitoshi; Ohyama, Katsumi; Liu, Hongpeng; Takeda, Yasuo; Oohira, Atsuhiko; Kawamura, Koki

doi:10.1002/(sici)1096-9861(19970602)382:2<141::aid-cne1>3.0.co;2-3

Cited by 127 publications

(60 citation statements)

References 56 publications

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“…Different from the CB + or CR + axons, L1 + axons also extended laterally into the LP/VP (Figures 4 and 9). L1 also is expressed by the growing thalamocortical axons (Fukuda et al, 1997), but these axons had not yet reached the ventral forebrain at the ages considered here. Thalamocortical L1 + axons in the posterior limb of the internal capsule are shown in Figure 5B.…”

Section: Resultsmentioning

confidence: 88%

Two separate subtypes of early non-subplate projection neurons in the developing cerebral cortex of rodents

Espinosa

2009

Front. Neuroanat.

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The preplate of the cerebral cortex contains projection neurons that connect the cortical primordium with the subpallium. These are collectively named pioneer neurons. After preplate partition, most of these pioneer neurons become subplate neurons. Certain preplate neurons, however, never associate with the subplate but rather with the marginal zone. In the present overview, we propose a novel classification of non-subplate pioneer neurons in rodents into two subtypes. In rats, the neurons of the first subtype are calbindin+ (CB), calretinin+ (CR) and L1+ and are situated in the upper part of the preplate before its partition. Neurons of the second subtype are TAG-1+ and are located slightly deeper to the previous population in the preplate. After the preplate partition, the CB+, CR+ and L1+ neurons remain in the marginal zone whereas TAG-1+ neurons become transiently localized in the upper cortical plate. In mice, by contrast, calcium binding proteins did not label pioneer neurons. We define in mice two subtypes of non-subplate pioneer neurons, either L1+ or TAG-1+/cntn2+. We propose these to be the homologues of the two subtypes of non-subplate pioneer neurons of rats. The anatomical distribution of these neuron populations is similar in rats and mice. The two populations of non-subplate pioneer neurons differ in their axonal projections. Axons of L1+ pioneer neurons project to the ganglionic eminences and the anterior preoptic area, but avoid entering the posterior limb of the internal capsule towards the thalamus. Axons of TAG-1+ pioneer neurons project to the lateral parts of the ganglionic eminences at the early stages of cortical histogenesis examined.

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Section: Resultsmentioning

confidence: 88%

Two separate subtypes of early non-subplate projection neurons in the developing cerebral cortex of rodents

Espinosa

2009

Front. Neuroanat.

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“…Next, wild type and NCAM null mutant E13.5 and E15.5 mice (Cremer et al, 1994) were immunolabeled with antibodies to NCAM or PSA along with L1, which labels TC axons (Chung et al, 1991; Fukuda et al, 1997; Demyanenko et al, 2011b). L1-immunoreactive axons expressed transmembrane NCAM isoforms, as detected with a monoclonal antibody (mAb P61) that recognizes the cytoplasmic domain of both NCAM140 and NCAM180 (Figures 2A–A” and 3A–A”; Gennarini et al, 1984), but not NCAM120.…”

Section: Resultsmentioning

confidence: 99%

Neural cell adhesion molecule, NCAM, regulates thalamocortical axon pathfinding and the organization of the cortical somatosensory representation in mouse

Enriquez-Barreto¹,

Palazzetti²,

Brennaman³

et al. 2012

Front. Mol. Neurosci.

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To study the potential role of neural cell adhesion molecule (NCAM) in the development of thalamocortical (TC) axon topography, wild type, and NCAM null mutant mice were analyzed for NCAM expression, projection, and targeting of TC afferents within the somatosensory area of the neocortex. Here we report that NCAM and its α-2,8-linked polysialic acid (PSA) are expressed in developing TC axons during projection to the neocortex. Pathfinding of TC axons in wild type and null mutant mice was mapped using anterograde DiI labeling. At embryonic day E16.5, null mutant mice displayed misguided TC axons in the dorsal telencephalon, but not in the ventral telencephalon, an intermediate target that initially sorts TC axons toward correct neocortical areas. During the early postnatal period, rostrolateral TC axons within the internal capsule along the ventral telencephalon adopted distorted trajectories in the ventral telencephalon and failed to reach the neocortex in NCAM null mutant animals. NCAM null mutants showed abnormal segregation of layer IV barrels in a restricted portion of the somatosensory cortex. As shown by Nissl and cytochrome oxidase staining, barrels of the anterolateral barrel subfield (ALBSF) and the most distal barrels of the posteromedial barrel subfield (PMBSF) did not segregate properly in null mutant mice. These results indicate a novel role for NCAM in axonal pathfinding and topographic sorting of TC axons, which may be important for the function of specific territories of sensory representation in the somatosensory cortex.

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“…4 c ). Lrp12/Mig13a -positive axons were immunopositive for antibodies against the cell adhesion molecule L1, a marker for commissural projections (Fukuda et al 1997; Jones et al 2002; Morante-Oria et al 2003) (Supplementary Fig. 3 b ).…”

Section: Resultsmentioning

confidence: 99%

Lrp12/Mig13a Reveals Changing Patterns of Preplate Neuronal Polarity during Corticogenesis that Are Absent in Reeler Mutant Mice

2010

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During corticogenesis, the earliest generated neurons form the preplate, which evolves into the marginal zone and subplate. Lrp12/Mig13a, a mammalian gene related to the Caenorhabditis elegans neuroblast migration gene mig-13, is expressed in a subpopulation of preplate neurons that undergo ventrally directed tangential migrations in the preplate layer and pioneer axon projections to the anterior commissure. As the preplate separates, Lrp12/Mig13a-positive neurons polarize in the radial plane and form a pseudocolumnar pattern, prior to moving to a deeper position within the emerging subplate layer. These changes in neuronal polarity do not occur in reeler mutant mice, revealing the earliest known defect in reeler cortical patterning and suggesting that the alignment of preplate neurons into a pseudolayer facilitates the movement of later-born radially migrating neurons into the emerging cortical plate.

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Immunohistochemical localization of neurocan and L1 in the formation of thalamocortical pathway of developing rats

Cited by 127 publications

References 56 publications

Two separate subtypes of early non-subplate projection neurons in the developing cerebral cortex of rodents

Two separate subtypes of early non-subplate projection neurons in the developing cerebral cortex of rodents

Neural cell adhesion molecule, NCAM, regulates thalamocortical axon pathfinding and the organization of the cortical somatosensory representation in mouse

Lrp12/Mig13a Reveals Changing Patterns of Preplate Neuronal Polarity during Corticogenesis that Are Absent in Reeler Mutant Mice

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