Immunohistochemical localization of human epidermal growth factor/.GAMMA.-urogastrone in submandibular glands and in their obstructive lesions.

Comparative ultrastructure of intercalated ducts in major salivary glands: a review

“…According to Tanaka et al (1984), these granules in the submandibular gland of adult female mice contain epidermal growth factor (EGF), but Gresik and Barka (1977) state that EGF is found exclusively in the granular convoluted tubules of the gland. However, immunohistochemical studies by Tsukitani et al (1987) and Tatemoto et al (1988) show that EGF is present in the ID cells of human submandibular glands. IDs in all human major salivary glands are strongly positive for transforming growth factor-alpha (Ogbereke et al, 1995); it is likely that this factor resides in the secretory granules.…”

Biophysiology of natriuretic peptides in the evolutionary varieties of vertebrate osmoregulatory systems from shark rectal to mammalian salivary glands

“…The kidney secretes hEGF. EFG originating from the kidney is called γ-urogastrone, and immunohistochemical evidence of γ-urogastrone was seen in centro-acinar cells and intercalated-striated duct of human salivary glands (67). Also, enzymes specific to growth factor synthesis were confined to secretory granules in GCT cells with the use of methoxynaphthylamine derivatives (68).…”

Widespread distribution of human epidermal growth factor(hEGF) in human tissues and organs under normal and tumorous conditions. Immunohistochemical studies with the use of polyclonal and monoclonal antibodies.

“…The preceding paper has reported the use of rabbit polyclonal anti-hEGF antiserum and monoclonal antibody, both raised against hEGF obtained by the synthetic gene method to detect hEGF in normal human salivary glands and their neoplastic lesions (53), and hEGF staining in pleomorphic adenomas have already been reported in detail (36,53,56). The immunohistochemically defined location of hEGF in salivary glands has been controversial; that is; hEGF has been detected in ductal segments (14,44,51,53,56) and in serous acinar compartments (23,27,41). The results are somewhat influenced by tissue fixation, and different antibodies used in these studies (51).…”

“…The immunohistochemically defined location of hEGF in salivary glands has been controversial; that is; hEGF has been detected in ductal segments (14,44,51,53,56) and in serous acinar compartments (23,27,41). The results are somewhat influenced by tissue fixation, and different antibodies used in these studies (51).…”

“…It is well known that mouse EGF (mEGF) is synthesized and accumulated in granular convoluted tubule cells of the submaxillary glands and that its synthesis is controlled by adrenergic agents (4). Urogastrone, which is isolated from human urine, has similar biological actions (9,47) and has been localized immunohistochemically in the Brunner's gland of duodenum and in salivary glands (14,23,51). Urogastrone and human EGF (hEGF) are biologically equivalent; that is, they are probably the same polypeptide and chemically identical (19).…”