Immunohistochemical localization of epidermal growth factor in rodent submandibular glands.

Mori, Masahiko; Hamada, Kenichi; Naito, Ryoji; Tsukitani, Kouji; Asano, Kenichi

doi:10.1267/ahc.16.536

Cited by 43 publications

(33 citation statements)

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“…Stem cells within the intercalated ducts can differentiate into amylase-producing acinar cells to aid recovery of gland function after radiation injury and after ductal ligation-induced gland atrophy (Lombaert et al, 2008;Cotroneo et al, 2010). Additionally, ductal cells of rodent salivary glands also serve as reservoirs of growth hormones, known to play a physiological role during development (Barka, 1980;Mori et al, 1983;Kato et al, 1991) Growth hormones stimulate cells to proliferate and may play a role in tissue regeneration. Therefore, apart from fluid-producing acinar elements, the protection of ductal elements can also favor the restoration of salivary function after injury in rodents.…”

Section: Aav9-tlk1b Protects Against Ir-mediated Xerostomiamentioning

confidence: 99%

Recombinant AAV9-TLK1B Administration Ameliorates Fractionated Radiation-Induced Xerostomia

Shanmugam

Dayton²,

Palaniyandi³

et al. 2013

Human Gene Therapy

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Salivary glands are highly susceptible to radiation, and patients with head and neck cancer treated with radiotherapy invariably suffer from its distressing side effect, salivary hypofunction. The reduction in saliva disrupts oral functions, and significantly impairs oral health. Previously, we demonstrated that adenoviralmediated expression of Tousled-like kinase 1B (TLK1B) in rat submandibular glands preserves salivary function after single-dose ionizing radiation. To achieve long-term transgene expression for protection of salivary gland function against fractionated radiation, this study examines the usefulness of recombinant adeno-associated viral vector for TLK1B delivery. Lactated Ringers or AAV2/9 with either TLK1B or GFP expression cassette were retroductally delivered to rat submandibular salivary glands (10 11 vg/gland), and animals were exposed, or not, to 20 Gy in eight fractions of 2.5 Gy/day. AAV2/9 transduced predominantly the ductal cells, including the convoluted granular tubules of the submandibular glands. Transgene expression after virus delivery could be detected within 5 weeks, and stable gene expression was observed till the end of study. Pilocarpine-stimulated saliva output measured at 8 weeks after completion of radiation demonstrated >10-fold reduction in salivary flow in saline-and AAV2/9-GFP-treated animals compared with the respective nonirradiated groups (90.8% and 92.5% reduction in salivary flow, respectively). Importantly, there was no decrease in stimulated salivary output after irradiation in animals that were pretreated with AAV2/9-TLK1B (121.5% increase in salivary flow; p < 0.01). Salivary gland histology was better preserved after irradiation in TLK1B-treated group, though not significantly, compared with control groups. Single preemptive delivery of AAV2/9-TLK1B averts salivary dysfunction resulting from fractionated radiation. Although AAV2/9 transduces mostly the ductal cells of the gland, their protection against radiation assists in preserving submandibular gland function. AAV2/9-TLK1B treatment could prove beneficial in attenuating xerostomia in patients with head and neck cancer undergoing radiotherapy.

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Section: Aav9-tlk1b Protects Against Ir-mediated Xerostomiamentioning

confidence: 99%

Recombinant AAV9-TLK1B Administration Ameliorates Fractionated Radiation-Induced Xerostomia

Shanmugam

Dayton²,

Palaniyandi³

et al. 2013

Human Gene Therapy

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“…GCT cells in rodent SMGs were found in mice, rats and hamsters, and EGF also exists in the rat GCT cells, however, the biological roles of hamster GCT cells are not yet understood. As compared with other rodent SMGs, GCT cells in matured mice were the most well developed, as well as rich in biological active peptides, whereas keratin proteins did not exist in these granules (20) and the hamster GCT did not contain many polypeptides (11). However, the vimentin type of filament proteins had a significantly strong reaction, in addition to filament proteins of desmin, actin, filamin and myosin which were slightly positive in hamster GCT cells.…”

Section: Discussionmentioning

confidence: 82%

“…In GCT cells in murine SMGs (mouse, rat, and hamster) particular structures exist for biosynthesis and storage of salivary gland growth factors (EGF and NGF), renin, kallikrein and esteroprotease (1,7,8,12,18). The preceding papers have reported that ductal segments of hamster SMGs were crossreacted with mouse EGF (11) and slightly positive to keratin proteins from immunohistochemical method (20). However, filament protein localizations excluded in keratins in hamster salivary glands as well as rodent glands have not been pointed out previously.…”

Section: Immunocytochemicalmentioning

confidence: 99%

Vimentin characteristically exists in granular convoluted tubules of hamster submandibular glands. Immunohistochemical studies on comparative localization of filament proteins in salivary gland ducts.

Mori

Takai

Hosaka

et al. 1985

Acta Histochem. Cytochem

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Immunocytochemicaldetection of vimentin class of filament proteins except for keratin is reported in ductal segments of rodent submandibular glands (SMGs) and compared to stainabilities of different ductal systems; intercalated duct (ICD), granular convoluted tubule (GCT), striated duct (SD) and excretory duct (ED), and in the major salivary glands; parotid (PG), submandibular (SMG) and sublingual glands (SLG). Filament proteins; vimentin, desmin, filamin, actin and myosin were examined by immunocytochemical technique. Vimentin characteristically was confined to the GCT cells of hamster SMGs, and was distributed in luminal surface of the SD cells of guinea pigs, where it was negative in the SMGs of rats and mice, but positive in SLG duct cells. Vimentin was also positive in the ICD cells of hamster SLG.Desmin was slightly positive in the duct cells of hamsters, and intensely positive in luminal side of the SD cells of SMG and PG in guinea pigs, and of SLG in rats. Trace amounts of desmin were also noted in the GCT cells of mice and rats. Filamin was strongly positive in smooth muscles of blood vessels and trace or slightly positive in ductal cells from all the specimens.Actin was also strongly evident in smooth muscles, and slightly reactive to hamsters GCT cells, guinea pigs, and rats ductal cells and was negative in mice. Myosin was slightly positive in the GCT cells of hamsters and in the SD cells of guinea pig.

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“…The biological significances of rodent submandibular glands have been focused on the biological active polypeptide producing system in GCT cells which showed sex dimorphism (1,6,16,22,23). In postnatal salivary glands, GCT cells were not developed just after birth; GCT cells appeared at 15 to 20 days of age in the mouse and 30 to 40 days of age in the rat.…”

Section: Discussionmentioning

confidence: 99%

“…(25) also noted that GCT cell development in the rat submandibular gland started in the 28th day and increased until the 84th day, and EGF appeared in GCT cells on the 25th day (23). In mouse submandibular glands, histologic features during postnatal stage have revealed that GCT cells develop on the 15th day and expressed presence of EGF and NGF (5,16). There are no detailed reports concerning the histology during the development of the sublingual gland .…”

Section: Electrophoresis and Immunoblottingmentioning

confidence: 99%

Immunohistochemical expression of chromogranin in postnatal developmental salivary glands of mice and rats.

Kunikata

Yamada

Ogata

et al. 1992

Acta Histochem. Cytochem

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The immunoreactivity of chromogranin was studied in postnatal salivary glands of the mice and rats. Chromogranin was observed in proacinar cells in both sexes during the postnatal developmental stage of the submandibular glands; the strongest reactivity was seen in 4th to 7th day specimens of mouse, and in 7th to 21st day specimens of rat. Acinar cells of mouse parotid glands showed positive staining in 1 to 42 days. No chromogranin staining was seen in granular convoluted tubule (GCT) cells of the submandibular glands in mice and rats. In sublingual glands of rat, reactivity of chromogranin was found in undifferentiated serous acinar cells, whereas in mice, it was absent in these cells. No sex difference of chromogranin staining was observed in submandibular glands in mice and rats. Immunoblotting of chromogranin using polyclonal anti-bovine SP-1/chromogranin antiserum showed in submandibular glands and adrenal glands of the rat consisted of chromogranin A, B and C. The results of this study suggest that the existence of chromogranin in proacinar cells of salivary glands is probably associated with the developmental phenomenon during the postnatal development.

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Immunohistochemical localization of epidermal growth factor in rodent submandibular glands.

Cited by 43 publications

References 0 publications

Recombinant AAV9-TLK1B Administration Ameliorates Fractionated Radiation-Induced Xerostomia

Recombinant AAV9-TLK1B Administration Ameliorates Fractionated Radiation-Induced Xerostomia

Vimentin characteristically exists in granular convoluted tubules of hamster submandibular glands. Immunohistochemical studies on comparative localization of filament proteins in salivary gland ducts.

Immunohistochemical expression of chromogranin in postnatal developmental salivary glands of mice and rats.

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