Immunohistochemical Localization of Elastic System Fibres in the Canine Prostate

Marettová, E.

doi:10.1515/fv-2017-0001

Cited by 6 publications

(7 citation statements)

References 11 publications

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“…Between more separated alveoli, the elastic fibres formed The elastic fibres in the mammary gland were seen to be much more dense than those observed in other exocrine glands [9,10,11] as was previously supposed. Elastic fibres form bridges between cells and their matrix [12].…”

Section: Elastinsupporting

confidence: 48%

The Distribution of Collagen and Elastic Fibres in the Lactating Bovine Mammary Gland

Marettová

Maretta

2019

Folia Veterinaria

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Collagen and elastic fibres are generally present in organs whose normal function requires great resistance and elasticity. The aim of this study was to localize the collagen and elastic fibres in the stroma of the bovine lactating mammary gland and to determine their role in the process of milk ejection. For this purpose, the histochemical staining for collagen and the immunohistochemical method for the detection of elastin were used. The accumulation of scattered collagen fibres was observed between and inside the lobules where they formed distinct septa. Between secretory alveoli, the collagen fibres were found to be concentrated into two incomplete layers surrounding the blood capillaries. Bundles of elastic fibres in high density were located in the interlobular spaces. A dense network of elastic fibres was located between adjacent alveoli. Elastic membranes were located beneath the secretory epithelium. The high concentration of the collagen and elastic fibres indicated, that both types of fibres play a significant role in the resistance during the secretory stage and in the recoil of the mammary gland after milk ejection.

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Section: Elastinsupporting

confidence: 48%

The Distribution of Collagen and Elastic Fibres in the Lactating Bovine Mammary Gland

Marettová

Maretta

2019

Folia Veterinaria

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“…We found a normal pattern [37] of elastic fibers in canine prostate (in the septa dividing the lobules, around the alveoli, ducts and the urethra). The interesting result was thatcanine PC, had a statistical higher median of elastin fibers (p=0.00229) than normal tissue.…”

Section: Discussionmentioning

confidence: 61%

“…An interesting finding was that solid canine PC had lower/absent Coll-IV expression than other PC patterns and normal tissues. Sinha et al, [37] found that Coll-IV immunostaining was less uniform or absent in the BM of poorly-differentiated human PC when compared to well-differentiated PC, HBP and normal human prostates. Similar results were also observed in feline and canine mammary tumors as well as in canine hemangiosarcoma [25,26].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Collagens Fibers (I, III, IV) and Elastin in the Extracellular Matrix of Normal and Neoplastic Canine Prostatic Tissues

Calderón¹,

Kobayashi²,

Vasconcelos³

et al. 2019

Preprint

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Collagen (Coll) is the most common protein in the extracellular matrix, responsible for providing tissue structure and support. In some types of cancer, including prostate cancer (PC) Coll deregulation was described and related to tumor progression and metastasis. This study aimed to investigate Coll-I, III, IV and elastin in canine normal prostate and PC, using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle and 5% around in blood vessels, in both normal prostate and PC. Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and never fibers of normal and PC samples. Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples. A high amount of elastic fibers was observed around the ducts and the urethra in normal and PC. The distribution and area percentage of staining for collagen are similar in normal and neoplastic canine prostate when analyzed with PSR and IHC.

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“…We found a normal pattern [51] of elastic fibers in canine prostate (in the septa dividing the lobules, around the alveoli, ducts and the urethra). The interesting result was that canine PC, had a statistical higher median of elastin fibers ( p = 0.00229) than normal tissue.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Collagen Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues

Calderón

Kobayashi

Vasconcelos

et al. 2019

Veterinary Sciences

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This study aimed to investigate collagen (Coll-I, III, IV) and elastin in canine normal prostate and prostate cancer (PC) using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle, and 5% surrounding blood vessels, in both normal prostate and PC. There was a higher median area of Coll-III in PC when compared to normal prostatic tissue (p = 0.001 for PSR and p = 0.05 for IHC). Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and nerve fibers of normal and PC samples. Although there was no difference in Coll-IV area between normal tissue and PC, tumors with Gleason score 10 showed absence of Coll-IV, when compared to scores 6 and 8 (p = 0.0095). Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples and were statistically higher in PC compared to normal tissue (p = 0.00229). Investigation of ECM components brings new information and should be correlated with prognosis in future studies.

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Immunohistochemical Localization of Elastic System Fibres in the Canine Prostate

Abstract: ABSTRACT

Cited by 6 publications

References 11 publications

The Distribution of Collagen and Elastic Fibres in the Lactating Bovine Mammary Gland

The Distribution of Collagen and Elastic Fibres in the Lactating Bovine Mammary Gland

Characterization of Collagens Fibers (I, III, IV) and Elastin in the Extracellular Matrix of Normal and Neoplastic Canine Prostatic Tissues

Characterization of Collagen Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues

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