Immunohistochemical localization of dentin matrix protein 1 in human dentin

Abstract

Dentin matrix protein 1 (DMP1) is a non-collagenous matrix protein with a recognized role in the formation of mineralized tissues such as dentin. The aim of this study was to analyze the distribution of DMP1 in human dentin by means of immunofluorescence and high-resolution immunogold labeling. Fully developed, sound human dentin specimens were submitted to fluorescence labeling and post-embedding immunolabeling techniques with a rabbit polyclonal antihuman DMP1 antibody followed by corresponding fluorochrome-conjugated or gold-conjugated secondary antibodies. Both immunofluorescence and immunogold labeling showed an intense labeling associated with the peritubular dentin. In addition, at the ultrastructural level, there was also a moderate and diffuse immunoreaction over intertubular dentin, and a weak labeling within predentin which increased in density towards the mineralization front. This study suggests that in adult human teeth, like in rodents, DMP1 is prevalently concentrated at the level of peritubular dentin and this feature is preserved also in fully developed-teeth. These data are consistent with what has been observed in rodents and suggest that DMP1 plays a role in maintenance of the dentin tubular space.

Histochemistry as a unique approach for investigating normal and osteoarthritic cartilage

“…These techniques are used also for examining the biochemical composition of cartilage, ligament, tendon and other tissues. 38,39 Immunostaining can be used to identify types I, II, III collagen, glycoproteins, laminin, tenasin, and fibronectin in plastic- or paraffin-embedded specimens. Common macromolecules such as cartilage matrix protein, types I, II and III collagen, and proteoglycans have also been successfully localized in cartilage specimens.…”

Taking a deep look: modern microscopy technologies to optimize the design and functionality of biocompatible scaffolds for tissue engineering in regenerative medicine

“…Kinikoglu et al [67] analysed human oral mucosa equivalents and imaged the integrity of epithelial cell layers, lamina propria and basement membrane (figure 2c(V)). TEM was further used in ultra-structural analysis of carbon nanotube binding to breast cancer cells [95], of bone (nanosized HA crystal structure and interaction with collagen fibres [96]) and in the immunogold applications [97].…”

Imaging analysis of early DMP1 mediated dentine remineralization

“…Phosphoproteins have been localized in inter-tubular dentin of rodent teeth (Rahima et al, 1988) and DMP1 immunolocalized in rodents (Aguiar and Arana-Chavez, 2011; Massa et al, 2005; Ye et al, 2004) and permanent human molars (Orsini et al, 2008). Interestingly, endogenous phosphoproteins remaining in the dentin matrix scaffold (Figure 3) were not able to initiate mineral formation and deposition (control group) after 2 weeks.…”