Immunohistochemical localization of CYP1A, vitellogenin and Zona radiata proteins in the liver of swordfish (Xiphias gladius L.) taken from the Mediterranean Sea, South Atlantic, South Western Indian and Central North Pacific Oceans

Desantis, Salvatore; Corriero, Aldo; Cirillo, F.; Deflorio, M.; Brill, Richard W.; Griffiths, Matthew; Lopata, Andreas L.; Serna, J. M. de la; Bridges, C.R.; Kime, David E.; Metrio, G. De

doi:10.1016/j.aquatox.2004.10.005

Cited by 29 publications

(28 citation statements)

References 43 publications

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“…Evidence of estrogenic effects 'in the open sea' that can be directly compared to that in cod (and dab in the present study), comes from studies on swordfish and tuna. VTG production has been demonstrated in male swordfish from the Mediterranean and from the waters around South Africa (Desantis et al, 2005). Similar to the findings in dab and cod, there also appears to be a positive relationship between VTG levels and fish size in male tuna (Barucca et al, 2006).…”

Section: Comparison With Previous Studies On Estrogenic Eds On Marinesupporting

confidence: 70%

“…Ocean (Desantis et al, 2005). This finding is the strongest evidence yet that elevated VTG concentrations in male fish can be directly attributed to pollution.…”

Section: Evidence Against the Hypothesis That The Presence Of Vtg In supporting

confidence: 54%

“…DDT, PCB). This hypothesis is 3 supported by abnormal VTG production being found in males of two other top predators, tuna (Thunnus thynnus) and swordfish (Xiphias gladius), that inhabit pelagic environments even further from shore (Fossi, Casini, Ancora, Moscatelli, Ausili, & Notarbartolo-di-Sciara, 2001;Fossi, Casini, Marsili, Neri, Mori, Ancora et al, 2002;De Metrio, Corriero, Desantis, Zubani, Cirillo, Deflorio et al, 2003;Fossi, Casini, Marsili, Ancora, Mori, Neri et al, 2004;Desantis, Corriero, Cirillo, Deflorio, Brill, Griffiths et al, 2005). Furthermore, in tuna, it also appears that the larger fish have higher VTG production (Barucca, Canapa, Olmo, & Regoli, 2006).…”

Section: Introductionmentioning

confidence: 79%

“…There were highly significant site differences in male swordfish, with no affected fish being found in the Pacific Ocean (Desantis et al, 2005). Similarly, male tuna caught in the Mediterranean had VTG in their plasma (Fossi et al, 2002) while tuna (Thunnus obesus) caught in the Pacific Ocean (Hashimoto, Kurihara, Strussmann, Yamasaki, Soyano, Hara et al, 2003) did not.…”

Section: Evidence Against the Hypothesis That The Presence Of Vtg In mentioning

confidence: 89%

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Evidence for estrogenic endocrine disruption in an offshore flatfish, the dab (Limanda limanda L.)

Scott

Sanders

Stentiford

et al. 2007

Marine Environmental Research

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Section: Comparison With Previous Studies On Estrogenic Eds On Marinesupporting

confidence: 70%

“…Ocean (Desantis et al, 2005). This finding is the strongest evidence yet that elevated VTG concentrations in male fish can be directly attributed to pollution.…”

Section: Evidence Against the Hypothesis That The Presence Of Vtg In supporting

confidence: 54%

Section: Introductionmentioning

confidence: 79%

Section: Evidence Against the Hypothesis That The Presence Of Vtg In mentioning

confidence: 89%

See 2 more Smart Citations

Evidence for estrogenic endocrine disruption in an offshore flatfish, the dab (Limanda limanda L.)

Scott

Sanders

Stentiford

et al. 2007

Marine Environmental Research

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“…Vg inmunoreactivity was detected in the liver of brown trout, Salmo trutta females, and no staining was observed in the liver of males (Wahli et al, 1998). Interestingly, as indicated previously in Mediterranean swordfish specimens (Fossi et al, 2001, 2007, Desantis et al, 2005, we observed positive Vg immunoreactivity in the liver of some male specimens (data not shown), which also presented high plasma oestradiol, Vg and Zrp concentrations, as well as high endocrine disrupting chemical levels (DDTs, PCBs, PAHs, etc) in the liver, gonads and plasma (Mori et al, 2005). The capacity of the liver of fish males to synthesise Zrp and Vg after exposure to natural estrogens or man-made chemicals has been widely reported (Oppen-Berntsen et al, 1992, Fossi et al, 2001, 2007Desantis et al, 2005).…”

Section: Discussionsupporting

confidence: 48%

Caracterización histoquímica de ovocitos del pez espada, <i>Xiphias gladius</i>

Ortiz-Delgado¹,

Porcelloni²,

Fossi³

et al. 2008

Sci. Mar.

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SUMMARY: This paper reports a histological, histochemical and immunohistochemical characterisation of growing oocytes of the swordfish Xiphias gladius. The presence and distribution of carbohydrates, proteins, lipids, calcium, iron, vitellogenin/ Vg, zona radiata protein/Zrp, metallothionein/Mt, and thyroid hormones/T 3 -T 4 were studied during oogenesis (cortical alveoli, globules, yolk-granules, cytoplasm, follicular and radiata envelopes). During the initial vitellogenic phase, the oocytes showed cortical alveoli and oil globules containing neutral lipids exclusively. During this phase, small yolk granules appeared around the peripheral cytoplasm, and they increased through exogenous vitellogenesis. Yolk granules were composed of glycoproteins, calcium, iron, and proteins rich in lysine, arginine, tyrosine, tryptophan, cysteine and cystine. Vg and Mt were immunohistochemically detected in yolk. The follicular envelope contained proteins rich in amino acids. Moreover, calcium and thyroid hormones (triiodotyronine and thyroxine/T 3 , T 4 ) were detected in this cell envelope. Cortical alveoli, which contained carboxylated and neutral glycoconjugates, were especially rich in N-acetyl-D-galactosamine, N-acetyl-Dglucosamine, galactose and sialic acid. Finally, the zona radiata was mainly proteinaceous in nature and was composed of calcium and neutral glycoproteins. The egg envelope or chorion and the liver showed specific immunoreactivities by using anti-salmon Zrp as the primary antiserum.Keywords: histochemistry, carbohydrates, lipids, glycoconjugates, vitellogenin, zona radiata protein, methallothionein, thyroid homones, cations, oocytes, Xiphias gladius. RESUMEN: Caracterización histoquímica de ovocitos del pez espada, Xiphias gladius. -En este trabajo se realiza una caracterización histológica, histoquímica e inmunohistoquímica de los ovocitos en crecimiento del pez espada, Xiphias gladius. Se estudia la presencia y distribución de carbohidratos, proteínas, lípidos, calcio, hierro, vitelogenina/Vg, proteína radiada/Zrp, metalotioneina/Mt y hormonas tiroideas/ T 3 -T 4 durante la ovogénesis (alveolos corticales, glóbulos, gránulos-vitelo, citoplasma, capas folicular y radiada). Durante la fase inicial de la vitelogénesis, los ovocitos presentan alveolos corticales y una gota lipídica conteniendo exclusivamente lípidos neutros. Durante esta fase en la periferia del citoplasma aparecen pequeños granulos de vitelo, incrementando progresivamente durante la vitelogénesis exógena. Los gránulos de vitelo están compuestos por glicoproteínas, calcio, hierro, proteínas ricas en lisina, arginina, tirosina, triptófano, cisteina y cistina, y contienen Vg y Mt. La capa folicular de los ovocitos del pez espada está compuesta por glicoproteínas y proteínas ricas en diferentes aminoácidos. Esta envuelta contiene calcio y hormonas tiroideas/ T 3 y T 4 . Los alveolos corticales están constituidos por glicoconjugados carboxilados y neutros, y son especialmente ricos en N-acetil-D-galactosamina, N-acetil-D-glucosamina, galactosa y...

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Fish Vitellogenin as a Biological Effect Marker of Oestrogenic Endocrine Disruption in the Open Sea

Scott

Robinson

2008

Advances in Fisheries Science

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Immunohistochemical localization of CYP1A, vitellogenin and Zona radiata proteins in the liver of swordfish (Xiphias gladius L.) taken from the Mediterranean Sea, South Atlantic, South Western Indian and Central North Pacific Oceans

Cited by 29 publications

References 43 publications

Evidence for estrogenic endocrine disruption in an offshore flatfish, the dab (Limanda limanda L.)

Evidence for estrogenic endocrine disruption in an offshore flatfish, the dab (Limanda limanda L.)

Caracterización histoquímica de ovocitos del pez espada, <i>Xiphias gladius</i>

Fish Vitellogenin as a Biological Effect Marker of Oestrogenic Endocrine Disruption in the Open Sea

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