Immunohistochemical Localization of Corticotropin-Releasing Factor in the Brain and Corticotropin-Releasing Factor and Thyrotropin-Stimulating Hormone in the Pituitary of Chinook Salmon (Oncorhynchus tshawytscha)

Matz, Stuart P.; Hofeldt, Gregory T.

doi:10.1006/gcen.1999.7253

Cited by 32 publications

(21 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Within 24 h of the hyperosmotic stress, 3-and 3.5-fold increases in CRF expression were observed in the preoptic area and hypothalamus, respectively. Similarly, previous studies in rainbow trout have observed an increase in preoptic area CRF expression in response to subordination (27) and handling stress (33) and identified the magnocellular portion of the preoptic nucleus (NPO) in trout and other salmonids as an important site of CRF gene expression (34) and CRF immunoreactive perikarya (34,35). The timing of the increases in forebrain CRF mRNA levels and return to control conditions corresponds with the transient increase and recovery in plasma osmolality.…”

Section: Sw Transfer and Forebrain Crf-related Peptide Gene Expressionmentioning

confidence: 71%

Differential Increase in Forebrain and Caudal Neurosecretory System Corticotropin-Releasing Factor and Urotensin I Gene Expression Associated with Seawater Transfer in Rainbow Trout

2005

View full text Add to dashboard Cite

Transfer to seawater (SW) in rainbow trout elicits an increase in plasma cortisol and a bout of anorexia. Although the corticotropin-releasing factor (CRF) system has known hypophysiotropic and anorexigenic properties, it is not known whether CRF-related peptides originating from either the forebrain or the caudal neurosecretory system (CNSS) play a role during SW acclimation. Therefore, we examined the effects of SW transfer on food intake, plasma osmolality, hypothalamic-pituitary-interrenal axis activity, and the expression of CRF and urotensin I (UI) in the forebrain and the CNSS. While SW transfer chronically suppressed food intake over a 2-wk period, it transiently increased plasma osmolality, ACTH, and cortisol. Similarly, 24 h after SW transfer, hypothalamic and preoptic area CRF mRNA levels were significantly increased but recovered to pretransfer levels within 7 d. Conversely, SW transfer elicited a delayed increase in hypothalamic UI mRNA levels and had no effect on preoptic area UI expression. In the CNSS, SW exposure was associated with parallel increases in CRF and UI mRNA levels from 24 h post transfer through 7 d. Finally, in situ hybridization demonstrated an extensive and overlapping pattern of CNSS CRF and UI expression. These results differentially implicate specific neuronal populations of the CRF system in the acute and chronic responses to a hyperosmotic stress and suggest that forebrain and CNSS CRF-related peptides have different roles in the coordinated response to fluid balance disturbances.

show abstract

Section: Sw Transfer and Forebrain Crf-related Peptide Gene Expressionmentioning

confidence: 71%

Differential Increase in Forebrain and Caudal Neurosecretory System Corticotropin-Releasing Factor and Urotensin I Gene Expression Associated with Seawater Transfer in Rainbow Trout

2005

View full text Add to dashboard Cite

show abstract

“…CRH has a widespread distribution in the teleostean brain; hypophysiotropic CRH-positive neurons have been identified in the NPO, and the nucleus lateralis tuberis (NLT) and nucleus recessi lateralis (NRL) in the hypothalamus (Matz & Hofeldt 1999, Pepels et al 2002, Huising et al 2004. Matz & Hofeldt (1999) showed that, in Chinook salmon (Oncorhynchus tshawytscha), CRH-immunoreactive fibers, originating from the preoptic region and NLT, terminate with boutonlike swellings in the proximity of TSH-ir cells in the pituitary pars distalis. These anatomical data, together with our results, provide evidence for the existence of a CRH-TSH-T 4 axis in teleosts.…”

Section: Discussionmentioning

confidence: 99%

Experimental hyperthyroidism and central mediators of stress axis and thyroid axis activity in common carp (Cyprinus carpio L.)

Geven¹,

Verkaar²,

Flik³

et al. 2006

Journal of Molecular Endocrinology

View full text Add to dashboard Cite

The effect of experimental hyperthyroidism, realized by T 4 injection, on central mediators of the hypothalamo-pituitaryinterrenal axis (HPI-axis) in common carp (Cyprinus carpio L.) was studied. Our results show that hyperthyroidism evokes a marked 3 . 2-fold reduction in basal plasma cortisol levels. Corticotropin-releasing hormone-binding protein (CRH-BP) mRNA levels in the hypothalamus, measured by real-time quantitative PCR, were significantly elevated by 40%, but CRH, urotensin-I, prepro-TRH, prohormone convertase-1 (PC1), and POMC mRNA levels were unchanged. In the pituitary pars distalis, PC1, CRH receptor-1, and POMC mRNA levels were unaffected, as was ACTH content. Plasma a-MSH concentrations were significantly elevated by 30% in hyperthyroid fish, and this was reflected in PC1 and POMC mRNA levels in pituitary pars intermedia that were increased 1 . 5-and 2 . 4-fold respectively. The a-MSH content of the pars intermedia was unchanged. Hyperthyroidism has profound effects on the basal levels of a central mediator, i.e., CRH-BP, of HPI-axis function in unstressed carp in vivo, and we conclude that HPI-and hypothalamo-pituitary-thyroidaxis functions are strongly interrelated. We suggest that the changes in plasma cortisol, thyroid hormone, and a-MSH levels reflect their concerted actions on energy metabolism.

show abstract

“…We also observed prominent CRH immunoreactivity within the PN fibre bundles that project onto the PI. Although many previous investigations have focussed on CRH immunoreactivity within the teleost rPD, considerable CRH immunoreactivity has also been reported within the PI of several species (Yulis & Lederis 1987, Matz & Hofeldt 1999, Pepels et al 2002. The large majority of the PI melanotropes innervated by these CRH-positive fibre bundles secrete -melanocyte-stimulating hormone ( -MSH) and contain various acetylated forms of -endorphin (van den Burg et al 2001).…”

Section: Innervation Of the Teleost Pituitary Glandmentioning

confidence: 99%

“…In Chinook salmon (Oncorhynchus tshawytscha), co-localisation of CRH-positive fibres and thyrotrophin (TSH)-secreting cells was reported (Matz & Hofeldt 1999). However, in carp no CRH immunoreactivity was observed in nerve fibres projecting onto the pPD, which contains the TSH-releasing cells.…”

Section: Innervation Of the Teleost Pituitary Glandmentioning

confidence: 99%

Structural characterisation of a cyprinid (Cyprinus carpio L.) CRH, CRH-BP and CRH-R1, and the role of these proteins in the acute stress response

Huising¹,

Metz²,

Schooten³

et al. 2004

Journal of Molecular Endocrinology

165

118

View full text Add to dashboard Cite

We elucidated the structure of the principle factors regulating the initiation of the acute stress response in common carp: corticotrophin-releasing hormone (CRH), CRH-receptor 1 (CRH-R1) and CRH-binding protein (CRH-BP). Phylogenetic analyses reveal that these proteins are evolutionarily well conserved in vertebrates. CRH and CRH-BP expression are not co-localised in the same hypothalamic perikarya. On the contrary, CRH-BP expression is limited to the perimeter of the nucleus preopticus (NPO), but is abundant in other regions, including an area directly rostral from, and in close proximity to, the NPO. Despite the lack of co-expression, the nerve fibres projecting onto both the rostral pars distalis (rPD) as well as the large fibre bundles projecting onto the pars intermedia (PI) contain CRH as well as CRH-BP, suggesting that both ACTH release from the rPD as well as the release of PI melanotrope content is regulated via CRH and CRH-BP. Finally, we show via real-time quantitative PCR that expression of hypothalamic CRH and CRH-BP following a 24 h restraint significantly increases, whereas PD CRH-R1 expression decreases; this reflects desensitisation of the PD for hypothalamic CRH output. We conclude that these factors are actively involved in the regulation of acute stress responses in the teleost fish.

show abstract

Immunohistochemical Localization of Corticotropin-Releasing Factor in the Brain and Corticotropin-Releasing Factor and Thyrotropin-Stimulating Hormone in the Pituitary of Chinook Salmon (Oncorhynchus tshawytscha)

Cited by 32 publications

References 39 publications

Differential Increase in Forebrain and Caudal Neurosecretory System Corticotropin-Releasing Factor and Urotensin I Gene Expression Associated with Seawater Transfer in Rainbow Trout

Differential Increase in Forebrain and Caudal Neurosecretory System Corticotropin-Releasing Factor and Urotensin I Gene Expression Associated with Seawater Transfer in Rainbow Trout

Experimental hyperthyroidism and central mediators of stress axis and thyroid axis activity in common carp (Cyprinus carpio L.)

Structural characterisation of a cyprinid (Cyprinus carpio L.) CRH, CRH-BP and CRH-R1, and the role of these proteins in the acute stress response

Contact Info

Product

Resources

About